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The Molecular Biology and Metabolism of Glutathione

  • Chapter

Part of the Plant Ecophysiology book series (KLEC,volume 2)

Keywords

  • Salicylic Acid
  • Glutathione Reductase
  • Glutathione Content
  • Brassica Juncea
  • Glutathione Synthesis

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Alfenito M.R., Souer E., Goodman C.D., Buell R., Mol J., Koes R., Walbot V. 1998. Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases.-Plant Cell 10: 1135–1149.

    PubMed  CrossRef  CAS  Google Scholar 

  • Amory A.M., Ford L., Pammenter N.W., Cresswell C.F. 1992. The use of 3-amino-l,2,4-triazole to investigate the short-term effects of oxygen toxicity on carbon assimilation by Pisum sativum seedlings.-Plant Cell. Environ. 15: 655–663.

    CAS  Google Scholar 

  • Aono M., Kubo A., Saji H., Tanaka K., Kondo N. 1993. Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity.-Plant Cell. Physiol. 34: 129–135.

    CAS  Google Scholar 

  • Arisi A.-C.M., Noctor G., Foyer CH., Jouanin L. 1997. Modification of thiol contents in poplars (Populus tremula x P. alba) over-expressing enzymes involved in glutathione biosynthesis.-Planta 203: 362–372.

    PubMed  CrossRef  CAS  Google Scholar 

  • Arisi A.C.M., Mocquot B., Lagriffoul A., Mench M., Foyer CH., Jouanin L. 2000. Responses to cadmium in leaves of transformed poplars overexpressing γ-glutamylcysteine synthetase.-Physiol. Plant. (submitted).

    Google Scholar 

  • Baier M., Dietz K.-J. 1997. The plant 2-Cys peroxiredoxin BAS1 is a nuclear-encoded chlorplast protein: its expressional regulation, phylogenetic origin, and implications for its specific physiological function in plants.-Plant J. 12: 179–190.

    PubMed  CrossRef  CAS  Google Scholar 

  • Bandyopadhyay S., Starke D.W., Mieyal J.J., Gronostajski R.M. 1998. Thioltransferase (glutaredoxin) reactivates the DNA-binding activity of oxidation-inactivated nuclear factor 1.-J. Biol. Chem. 273: 392–397.

    PubMed  CAS  Google Scholar 

  • Bartling D., Radzio R., Steiner U., Weiler E.W. 1993. A glutathione-S-transferase with glutathione peroxidase activity from Arabidopsis thaliana-molecular cloning and functional characterization.-Eur. J. Biochem. 216: 579–586.

    PubMed  CrossRef  CAS  Google Scholar 

  • Besson V., Neuburger M., Rebeille F., Douce R. 1995. Evidence for three serine hydroxy-methyltransferases in green leaf cells. Purification and characterization of the mitochondrial and chloroplastic isoforms.-Plant Physiol. Biochem. 33: 665–673.

    CAS  Google Scholar 

  • Bielawski W., Joy K.W. 1986. Reduced and oxidised glutathione and glutathione reductase activity in tissues of Pisum sativum.-Planta 169: 267–272.

    CrossRef  CAS  Google Scholar 

  • Brisson L.F., Zelitch I., Havir E.A. 1998. Manipulation of catalase levels produces altered photosynthesis in transgenic tobacco plants.-Plant Physiol. 116: 259–269.

    PubMed  CrossRef  CAS  Google Scholar 

  • Broadbent P., Creissen G.P., Kular B., Wellburn A.R., Mullineaux P. 1995. Oxidative stress responses in transgenic tobacco containing altered levels of glutathione reductase activity.-Plant J. 8: 247–255.

    CrossRef  CAS  Google Scholar 

  • Bunkelmann J.R., Trelease R.N. 1996. Ascorbate peroxidase. A prominent membrane protein in oilseed glyoxysomes.-Plant Physiol. 110: 589–598.

    PubMed  CrossRef  CAS  Google Scholar 

  • Burgener M., Suter M., Jones S., Brunold C. 1998. Cyst(e)ine is the transport metabolite of assimilated sulfur from bundle-sheath to mesophyll cells in maize leaves.-Plant Physiol. 116: 315–1322.

    CrossRef  Google Scholar 

  • Butt A.D., Ohlrogge J.B. 1991. Acyl carrier protein is conjugated to glutathione in spinach seed.-Plant Physiol. 96: 937–942.

    CAS  PubMed  Google Scholar 

  • Buwalda F., De Kok L., Stulen I., Kuiper P.J.C. 1988. Cysteine, γ-glutamylcysteine and glutathione contents of spinach leaves as affected by darkness and application of excess sulfur.-Physiol. Plant. 80: 196–204.

    Google Scholar 

  • Buwalda F., Stulen I., De Kok L.J., Duiper P.J.C. 1990. Cysteine, γ-glutamylcysteine and glutathione contents of spinach leaves as affected by darkness and application of excess sulfur. II. Glutathione accumulation in detached leaves exposed to H 2 S in the absence of light is stimulated by the supply of glycine to the petiole.-Physiol. Plant. 80: 196–204.

    CrossRef  CAS  Google Scholar 

  • Chamnongpol S., Willekens H., Langebartels C., Van Montagu M., Inzé D., Van Camp W. 1996. Transgenic tobacco with a reduced catalase activity develops necrotic lesions and induces pathogenesis-related expression under high light.-Plant J. 10: 491–503.

    CrossRef  CAS  Google Scholar 

  • Chamnongpol S., Willekens H., Moeder W., Langebartels C., Sandermann H., Van Montagu M., Inzé D., Van Camp W. 1998. Defense activation and enhanced pathogen tolerance induced by H 2 O 2 in transgenic tobacco.-Proc. Natl. Acad. Sci. USA 95: 5818–5823.

    PubMed  CrossRef  CAS  Google Scholar 

  • Chen J., Goldsbrough P.B. 1994. Increased activity of γ-glutamylcysteine synthetase in tomato cells selected for cadmium tolerance.-Plant Physiol. 106: 233–239.

    PubMed  CAS  Google Scholar 

  • Chen W., Chao G., Singh K.B. 1996. The promoter of a H 2 O 2 -inducible, Arabidopsis glutathione-S-transferase gene contains closely linked OBF-and OBP1-binding sites.-Plant J. 10: 955–966.

    PubMed  CrossRef  CAS  Google Scholar 

  • Choudhary A.D., Lamb C.J., Dixon R.A. 1990. Stress responses in Alfalfa (Medicago sativa L.) VI. Differential responsiveness of chalcone synthase induction to fungal elicitor or glutathione in electroporated protoplasts.-Plant Physiol. 94: 1802–1807.

    CAS  PubMed  Google Scholar 

  • Cobbett C.S., May M.J., Howden R., Rolls B. 1998. The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in γ-glutamylcysteine synthetase.-Plant J. 16: 73–78.

    PubMed  CrossRef  CAS  Google Scholar 

  • Creissen G., Edwards E.A., Enard C., Wellburn A., Mullineaux P. 1992. Molecular characterisation of glutathione reductase cDNAs from pea (Pisum sativum L.).-Plant J. 2: 129–131.

    PubMed  CAS  Google Scholar 

  • Creissen G.P., Mullineaux P.M. 1995. Cloning and characterization of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme.-Planta 197: 422–425.

    PubMed  CrossRef  CAS  Google Scholar 

  • Creissen G., Reynolds H., Xue Y.B., Mullineaux P. 1995. Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mito-chondria.-Plant J. 8: 167–175.

    PubMed  CrossRef  CAS  Google Scholar 

  • Creissen G., Firmin J., Fryer M., Kular B., Leyland N., Reynolds H., Pastori G., Wellburn F., Baker N., Wellburn A., Mullineaux P. 1999. Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress.-Plant Cell 11: 1277–1291.

    PubMed  CrossRef  CAS  Google Scholar 

  • Cummins I., Cole D.J., Edwards R. 1999. A role for glutathione transferases functioning as glutathione peroxidases in resistance to multiple herbicides in black grass.-Plant J. 18: 285–292.

    PubMed  CrossRef  CAS  Google Scholar 

  • Dafré A.L., Sies H., Akerboom T. 1996. Protein S-thiolation and regulation of microsomal glutathione transferase activity by the glutathione redox couple.-Arch. Biochem. Biophys. 332: 288–294.

    PubMed  Google Scholar 

  • Delledonne M., Xia Y., Dixon R.A.; Lamb C. 1998. Nitric oxide functions as a signal in plant disease resistance.-Nature 394: 585–588.

    PubMed  CAS  Google Scholar 

  • Demple B. 1998. Signal transduction-a bridge to control.-Science 279: 1655–1656.

    PubMed  CrossRef  CAS  Google Scholar 

  • Doulis A.G., Debian N., Kingston-Smith A.H., Foyer CH. 1997. Differential localization of antioxidants in maize leaves.-Plant Physiol. 114: 1031–1037.

    PubMed  CAS  Google Scholar 

  • Dron M., Clouse S.D., Dixon R.A., Lawton M.A., Lamb C.J. 1988. Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts.-Proc. Natl. Acad. Sci. USA 85: 6738–6742.

    CAS  PubMed  Google Scholar 

  • Du H., Klessig D.F. 1997. Role for salicylic acid in the activation of defense responses in catalase-deficient transgenic tobacco.-Mol. Plant-Microbe Interact. 10: 922–925.

    CAS  Google Scholar 

  • Edwards E.A., Rawsthorne S., Mullineaux P.M. 1990. Subcellular distribution of multiple forms of glutathione reductase in pea (Pisum sativum L.).-Planta 180: 278–284.

    CrossRef  CAS  Google Scholar 

  • Edwards R., Blount J.W., Dixon R.A. 1991. Glutathione and elicitation of the phytaolexin response in legume cultures.-Planta 184: 403–409.

    CrossRef  CAS  Google Scholar 

  • Eshdat Y., Holland D., Faltin Z., Ben-Hayyim G. 1997. Plant glutathione peroxidases.-Physiol. Plant. 100: 234–240.

    CrossRef  CAS  Google Scholar 

  • Feierabend J., Engel S. 1986. Photoinactivation of catalase in vitro and in leaves.-Arch. Biochem. Biophys. 251: 567–576.

    PubMed  CrossRef  CAS  Google Scholar 

  • Fordham-Skelton A.P., Skipsey M., Evans I.M., Edwards R., Gatehouse J.A. 1999. Higher plant tyrosine-specific phosphatases (PTPs) contain novel amino-terminal domains: expression during embryogenesis.-Plant Mol. Biol. 39: 593–605.

    PubMed  CrossRef  CAS  Google Scholar 

  • Foyer Ch., Halliwell B. 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism.-Planta 133: 21–25.

    CrossRef  Google Scholar 

  • Foyer Ch., Lelandais M., Galap C., Kunert K.-J. 1991. Effects of elevated cytosolic glutathione reductase activity on the cellular glutathione pool and photosynthesis in leaves under normal and stress conditions.-Plant Physiol. 97: 863–872.

    CAS  PubMed  Google Scholar 

  • Foyer Ch., Souriau N., Perret S., Lelandais M., Kunert K.J., Pruvost C., Jouanin L. 1995. Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees.-Plant Physiol. 109: 1047–1057.

    PubMed  CrossRef  CAS  Google Scholar 

  • Foyer Ch., Lopez-Delgado H., Dat J.F., Scott I.M. 1997. Hydrogen peroxide-and glutathione-associated mechanisms of acclimatory stress tolerance and signalling.-Physiol. Plant. 100: 241–254.

    CrossRef  CAS  Google Scholar 

  • Foyer Ch., Mullineaux P.M. 1998. The presence of dehydroascorbate and dehydroascorbate reductase in plant tissues.-FEBS Lett. 425: 528–529.

    PubMed  CrossRef  CAS  Google Scholar 

  • Foyer Ch., Noctor G. 2000. Oxygen processing in photosynthesis: regulation and signalling.-New Phytol. 146: 359–388.

    CrossRef  CAS  Google Scholar 

  • Frendo P., Gallesi D., Turnbull R., Van de Sype G., Hérouart D., Puppo A. 1999. Localisation of glutathione and homoglutathione in Medicago truncatula is correlated to a differential expression of genes involved in their synthesis.-Plant J. 17: 215–219.

    CrossRef  CAS  Google Scholar 

  • Godwin A.K., Meister A., O’Dwyer P.J., Huang C.S., Hamilton T.C., Anderson M.E. 1992. High resistance to cisplatin in human ovarian cancer cell lines is associated with marked increase of glutathione synthesis.-Proc. Natl. Acad. Sci. USA 89: 3070–3074.

    PubMed  CAS  Google Scholar 

  • Halliwell B., Foyer CH. 1978. Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography.-Planta 139: 9–17.

    CrossRef  CAS  Google Scholar 

  • Hell R., Bergmann L. 1988. Glutathione synthetase in tobacco suspension cultures: catalytic properties and localisation.-Physiol. Plant 72: 70–76.

    CAS  Google Scholar 

  • Hell R., Bergmann L. 1990.γ-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localisation.-Planta 180: 603–312.

    CAS  Google Scholar 

  • Heo J., Holbrook G.P. 1999. Regulation of 2-carboxy-D-arabinitol 1-phosphate phosphatase: activation by glutathione and interaction with thiol reagents.-Biochem. J. 338: 409–416.

    PubMed  CrossRef  CAS  Google Scholar 

  • Hérouart D., Van Montagu M., Inzé D. 1993. Redox-activated expression of the cytosolic copper/zinc superoxide dismutase gene in Nicotiana.-Proc. Natl. Acad. Sci. USA 90: 3108–3112.

    PubMed  Google Scholar 

  • Hertwig B., Streb P., Feirerabend J. 1992. Light dependence of catalase synthesis and degradation in leaves and the influence of interfering stress conditions.-Plant Physiol. 100: 1547–1553.

    CAS  PubMed  Google Scholar 

  • Huang C.S., Chang L.S., Anderson M.E., Meister A. 1993. Catalytic and regulatiory properties of the heavy subunit of rat kidney γ-glutamylcysteine synthetase.-J. Biol. Chem. 268: 19675–19680.

    PubMed  CAS  Google Scholar 

  • Ireland R.J., Hiltz D.A. 1990. Glycine and serine in non-photosynthetic tissues.-In: Wallsgrove R.M., Amino acids and their derivatives in higher plants, pp. 111–118.-Cambridge University Press, UK.

    Google Scholar 

  • Jahngen-Hodge J., Obin M.S., Gong X., Shang F., Nowell T.R., Gong J., Abasi H., Blumberg J., Taylor A. 1997. Regulation of ubiquitin-conjugating enzymes by glutathione following oxidative stress.-J. Biol. Chem. 272: 28218–28226.

    PubMed  CrossRef  CAS  Google Scholar 

  • Jiménez A., Hernández J.A., del Río L., Sevilla F. 1997. Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves.-Plant Physiol. 114: 275–284.

    PubMed  Google Scholar 

  • Ju G.C., Li X.Z., Rauser W.E., Oaks A. 1997. Influence of cadmium on the production of γ-glutamylcysteine peptides and enzymes of nitrogen assimilation in Zea mays seedlings.-Physiol. Plant. 101: 793–799.

    CrossRef  CAS  Google Scholar 

  • Kaiser W.M. 1979. Reversible inhibition of the Calvin cycle and activation of oxidative pentose phosphate cycle in isolated intact chloroplasts by hydrogen peroxide.-Planta 145: 377–382.

    CrossRef  CAS  Google Scholar 

  • Kaminaka H., Morita S., Nakjima M., Masumara T., Tanaka K. 1998. Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.).-Plant Cell Physiol. 39: 1269–1280.

    PubMed  CAS  Google Scholar 

  • Kendall A.C., Keys A.J., Turner J.C., Lea P.J., Miflin B.J. 1983. The isolation and characterisation of a catalase-deficient mutant of barley (Hordeum vulgare L.).-Planta 159: 505–511.

    CrossRef  CAS  Google Scholar 

  • Klapheck S., Latus C., Bergmann L. 1987. Localisation of glutathione synthetase and distribution of glutathione in leaf cells of Pisum sativum L.-J. Plant Physiol. 131: 123–131.

    CAS  Google Scholar 

  • Klapheck S. 1988. Homoglutathione: isolation, quantification and occurrence in legumes.-Physiol. Plant. 74: 727–732.

    CAS  Google Scholar 

  • Klapheck S., Chrost B., Starke J., Zimmermann H. 1992. γ-Glutamylcysteinylserine-a new homologue of glutathione in plants of the family Poaceae.-Bot. Acta 105: 174–179.

    CAS  Google Scholar 

  • Kranner I., Grill D. 1996. Significance of thiol-disulfide exchange in resting stages of plant development.-Bot. Acta 109: 8–14.

    CAS  Google Scholar 

  • Kubo A., Sano T., Saji H., Tanaka K., Kondo N., Tanaka K. 1993. Primary structure and properties of glutathione reductase from Arabidopsis thaliana.-Plant Cell Physiol. 34: 1259–1266.

    CAS  Google Scholar 

  • Kunert K.J., Cresswell C.F., Schmidt A., Mullineaux P.M., Foyer CH. 1990. Variations in the activity of glutathione reductase and the cellular glutathione content in relation to sensitivity to methyl viologen in Escherichia coli.-Arch. Biochem. Biophys. 282: 233–238.

    PubMed  CrossRef  CAS  Google Scholar 

  • Kunert K.J., Foyer C. 1993. Thiol/disulfide exchange in plants.-In: Sulfur nutrition and assimilation in higher plants, pp. 139–151.-SPB Academic Pub., The Hague.

    Google Scholar 

  • Kurokawa H., Ishida T., Nishio K., Arioka H., Sata M., Fukumoto H., Miura M., Saijo N. 1995. γ-Glutamylcysteine synthetase gene overexpression results in increased activity of the ATP-dependent glutathione S-conjugate export pump and cisplatin resistance.-Biochem. Biophys. Res. Commun. 216: 258–64.

    PubMed  CrossRef  CAS  Google Scholar 

  • Lacuesta M., Dever L.V., Muñoz-Rueda A., Lea P.J. 1997. A study of photorespiratory ammonia production in the C4 plant Amaranthus edulis, using mutants with altered photosynthetic capacities.-Physiol. Plant. 99: 447–455.

    CrossRef  CAS  Google Scholar 

  • Lamoureux G.L., Rusness D.G. 1993. Glutathione in the metabolism and detoxification of xenobiotics in plants.-In: De Kok L.J., Stulen I., Rennenberg H., Brunold C., Rauser W.E. (Eds.), Sulfur nutrition and assimilation in higher plants. Regulatory, agricultural and environmental aspects, pp. 221–237.-Academic Publishing, The Hague.

    Google Scholar 

  • Lapperre T.S., Jimenez L.A., Antonicelli F., Drost E.M., Hiemstra P.S., Stolk J., MacNee W., Rahman I. 1999. Apocynin increases glutathione synthesis and activates AP-1 in alveolar epithelial cells.-FEBS Lett. 443: 235–239.

    PubMed  CrossRef  CAS  Google Scholar 

  • Law M.Y., Halliwell B. 1986. Purification and properties of glutathione synthetase from spinach (Spinacia oleracea) leaves.-Plant Sci. 43: 185–191.

    CrossRef  CAS  Google Scholar 

  • Lawton M.A., Clouse S.D., Lamb C.J. 1990. Glutathione-elicited changes in chromatin structure within the promoter of the defense gene chalcone synthetase.-Plant Cell Reports 8: 561–564.

    CrossRef  CAS  Google Scholar 

  • Lee H., Jo J., Son D. 1998. Molecuular cloning and characterization of the gene encoding glutathione reductase inBrassicacampestris.-Biochem. Biophys. Acta 1395: 309–314.

    PubMed  CAS  Google Scholar 

  • Levine A., Tenhaken R., Dixon R., Lamb C. 1994. H 2 O 2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response.-Cell 79: 1–20.

    CrossRef  Google Scholar 

  • Li Z.-S., Zhao Y., Rea P.A. 1995. Magnesium adenosine 5’-triphosphate-energized transport of glutathione S-conjugates by plant vacuolar membrane vesicles.-Plant Physiol. 107: 1257–1268.

    PubMed  CAS  Google Scholar 

  • Li Z.-S., Alfenito M., Rea P.A., Walbot V., Dixon R.A. 1997. Vacuolar uptake of the phytoalexin medicarpin by the glutathione conjugate pump.-Phytochem. 45: 689–693.

    CAS  Google Scholar 

  • Lieberman M.W., Weisman A.L., Shi Z.Z., Carter B.Z., Barrios R., Ou C.N., Chévez-Barrios P., Wang Y., Habib G.M., Goodman J.C., Huang S.L., Lebovitz R.M., Matzuk M.M. 1996. Growth retardation and cysteine deficiency in γ-glutamyl transpeptidasedeficient mice.-Proc. Natl. Acad. Sci. USA 93: 7923–7926.

    PubMed  CAS  Google Scholar 

  • Macnicol P.K. 1987. Homoglutathione and glutathione synthetases of legume seedlings: partial purification and substrate specificity.-Plant Sci. 53: 229–35.

    CrossRef  CAS  Google Scholar 

  • Mannervik B., Danielson U.H. 1988. Glutathione transferases-structure and catalytic activity.-CRC Crit. Rev. Biochem. 23: 283–337.

    PubMed  CAS  Google Scholar 

  • Marrs K.A., Alfenito M.R., Lloyd A.M., Walbot V. 1995. A glutathione S-transferase involved in vacuolar transfer encoded by the maize gene Bronze2.-Nature 375: 397–400.

    PubMed  CrossRef  CAS  Google Scholar 

  • Marrs K.A. 1996. The functions and regulation of glutathione S-transferases in plants.-Annu. Rev. Plant Physiol. Plant. Mol. Biol. 47: 127–158.

    PubMed  CrossRef  CAS  Google Scholar 

  • Martinoia E., Grill E., Tommasini R., Kreuz K., Amrhein N. 1993. An ATP-dependent glutathione-S-conjugate ‘export’ pump in the vacuolar membrane of plants.-Nature 364: 247–249.

    CrossRef  CAS  Google Scholar 

  • Mathieu C. 1999. Formation d’espèces radicalaires et de défense antioxydante dans les nodosités de Légumineuses: mécanismes délétères liés à la sénescence et étude moléculaire des enzymes de synthèse du glutathion et du homoglutathion.-PhD thesis, Université de Nice-Sophia Antipolis, France.

    Google Scholar 

  • May M.J., Leaver C.J. 1993. Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures.-Plant Physiol. 130: 621–627.

    Google Scholar 

  • May M. J., Leaver CJ. 1994. Arabidopsis thaliana γ-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast and E. coli homologs.-Proc. Natl. Acad. Sci. USA 91: 10059–10063.

    PubMed  CAS  Google Scholar 

  • May M.J., Hammond-Kosack K.E., Jones J.D.G. 1996. Involvement of reactive oxygen species, glutathione metabolism, and lipid peroxidation in the Cf-gene-dependent defense response of tomato cotyledons induced by race-specific elicitors of Cladosporiumfulvum.-Plant Physiol. 110: 1367–1379.

    PubMed  CAS  Google Scholar 

  • May M. J., Vernoux T., Leaver C., van Montagu M., Inzé D. 1998a. Glutathione homeostasis in plants: implications for environmental sensing and plant development.-J. Exp. Bot. 49: 649–667.

    CrossRef  CAS  Google Scholar 

  • May M.J., Vernoux T., Sánchez-Fernández R., van Montagu M., Inzé D. 1998b. Evidence for posttranscriptional activation of γ-glutamylcysteine synthetase during plant stress responses.-Proc. Natl. Acad. Sci. USA 95: 12049–12054.

    PubMed  CrossRef  CAS  Google Scholar 

  • Meister A. 1988. Glutathione metabolism and its selective modification.-J. Biol. Chem. 263: 17205–17208.

    PubMed  CAS  Google Scholar 

  • Meuwly P., Thibault P., Rauser W.E. 1993. γ-Glutamylcysteinylglutamic acid-a new homologue of glutathione in maize seedlings exposed to cadmium.-FEBS Lett. 336: 472–476.

    PubMed  CrossRef  CAS  Google Scholar 

  • Moorhead G., Douglas P., Cotelle V., Harthill J., Morrice N., Meek S., Deiting U., Stitt M., Scarabel M., Aitken A., MacKintosh C. 1999. Phosphorylation-dependent interactions between enzymes of plant metabolism and 14-3-3 proteins.-Plant J. 18: 1–12.

    PubMed  CrossRef  CAS  Google Scholar 

  • Mulcahy R.T., Bailey H.H., Gipp J.J. 1994. Up-regulation of γ-glutamylcysteine synthetase activity in melphalan-resistant human multiple myeloma cells expressing increased glutathione levels.-Cancer Chemother. Pharmacol. 34: 67–71.

    PubMed  CAS  Google Scholar 

  • Mulcahy R.T., Bailey H.H., Gipp J.J. 1995. Transfection of complementary DNAs for the heavy and light subunits of human γ-glutamylcysteine synthetase results in an elevation of intracellular glutathione and resistance to melphalan.-Cancer Res. 55: 4771–75.

    PubMed  CAS  Google Scholar 

  • Mullineaux P.M., Karpinski S., Jimenez A., Cleary S.P., Robinson C., Creissen G.P. 1998. Indentification of cDNAs encoding plastid-targeted glutathione peroxidase.-Plant J. 13: 375–379.

    PubMed  CrossRef  CAS  Google Scholar 

  • Noctor G., Strohm M., Jouanin L., Kunert K.J., Foyer CH., Rennenberg H. 1996. Synthesis of glutathione in leaves of transgenic poplar (Populus tremula x P. alba) overexpressing γ-glutamylcysteine synthetase.-Plant Physiol. 112: 1071–78.

    PubMed  CAS  Google Scholar 

  • Noctor G., Jouanin L., Arisi A.-C.M., Valadier M.-H., Roux Y., Foyer CH. 1997a. Lightdependent modulation of foliar glutathione synthesis and associated amino acid metabolism in transformed poplar.-Planta 202: 357–369.

    CrossRef  CAS  Google Scholar 

  • Noctor G., Arisi A.-C.M., Jouanin L., Valadier M.-H., Roux Y., Foyer CH. 1997b. The role of glycine in determining the rate of glutathione synthesis in poplars. Possible implications for glutathione production during stress.-Physiol. Plant. 100: 255–263.

    CrossRef  CAS  Google Scholar 

  • Noctor G., Arisi A.-C.M., Jouanin L., Foyer CH. 1998a. Manipulation of glutathione and amino acid biosynthesis in the chloroplast.-Plant Physiol. 118: 471–482.

    PubMed  CrossRef  CAS  Google Scholar 

  • Noctor G., Arisi A.-C.M., Jouanin L., Kunert K.-J., Rennenberg H., Foyer CH. 1998b. Glutathione: biosynthesis and metabolism explored in transformed poplar.-J. Exp. Bot 49: 623–647.

    CAS  Google Scholar 

  • Noctor G., Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control.-Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 249–279.

    PubMed  CrossRef  CAS  Google Scholar 

  • Noctor G., Arisi A.C.M., Jouanin L., Foyer CH. 1999. Photorespiratory glycine enhances glutathione accumulation in both the chloroplastic and cytosolic compartments.-J. Exp. Bot. 50: 1157–1167.

    CrossRef  CAS  Google Scholar 

  • Noctor G., Veljovic-Jovanovic S., Foyer CH. 2000. Peroxide processing in photosynthesis: antioxidant coupling and redox sigalling.-Phil. Trans. R. Soc. Lond. B 355: 1465–1475.

    CAS  Google Scholar 

  • Ocheretina O., Scheibe R. 1994. Cysteines of chloroplast NADP-malate dehydrogenase form mixed disulfides.-FEBS Lett. 355: 254–258.

    PubMed  CrossRef  CAS  Google Scholar 

  • Pastori G.M., Trippi V.S. 1992. Oxidative stress induces high rate of glutathione reductase synthesis in a drought-resistant maize strain.-Plant Cell Physiol. 33: 957–961.

    CAS  Google Scholar 

  • Pastori G., Mullineaux P., Foyer CH. 2000. Post-transcriptional regulation prevents accumulation of glutathione reductase protein and activity in the bundle sheath cells of maize.-Plant Physiol. 122: 667–675.

    PubMed  CrossRef  CAS  Google Scholar 

  • Pilon-Smits E.A.H., Ahu Y.L., Pilon M., Terry N. 1999. Overexpression of glutathione synthesizing enzymes enhances cadmium accumulation in Brassica juncea.-In: Proc. 5th Intern. Conf. on the Biogeochem. of Trace Elements, Vienna 1999, pp. 890–891.

    Google Scholar 

  • Price C.A. 1957. A new thiol in legumes.-Nature 180: 148–149.

    PubMed  CAS  Google Scholar 

  • Rahman I., Smith C.A.D., Lawson M.F., Harrison D.J., MacNee W. 1996. Induction of γ-glutamylcysteine synthetase by cigarette smoke is associated with AP-1 in human alveolar epithelial cells.-FEBS Lett. 396: 21–25.

    PubMed  CrossRef  CAS  Google Scholar 

  • Rao M.V., Davis K.R. 1999. Ozone-induced cell death occurs via two distinct mechanisms in Arabidopsis: the role of salicylic acid.-Plant J. 17: 603–614.

    PubMed  CrossRef  CAS  Google Scholar 

  • Rasmusson A.G., Møller I.M. 1990. NADP-utilizing enzymes in the matrix of plant mitochondria.-Plant Physiol. 94: 1012–1018.

    CAS  PubMed  Google Scholar 

  • Rawlins M.R., Leaver C.J., May M.J. 1995. Characterisation of an Arabidopsis thaliana cDNA encoding glutathione synthetase.-FEBS Lett. 376: 81–86.

    PubMed  CrossRef  CAS  Google Scholar 

  • Rawlins M.R. 1998. Glutathione synthetase in Arabidopsis thaliana.-PhD thesis, St Catherine’s college, University of Oxford, UK.

    Google Scholar 

  • Roxas V.P., Smith R.K., Allen E.R., Allen R.D. 1997. Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress.-Nat. Biotechnol. 15: 988–991.

    PubMed  CrossRef  CAS  Google Scholar 

  • Rüegsegger A., Brunold C. 1992. Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings.-Plant Physiol. 99: 428–433.

    PubMed  Google Scholar 

  • Rüegsegger A., Brunold C. 1993. Localisation of γ-glutamylcysteine syntetase and glutathione synthetase activity in maize seedlings.-Plant Physiol. 101: 561–566.

    PubMed  Google Scholar 

  • Sánchez-Fernández R., Fricker M., Corben L.B., White N.S., Sheard N., Leaver C.J., Van Montagu M., Inzé D., May M. J. 1997. Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control.-Proc. Natl. Acad. Sci. USA 94: 2745–2750.

    PubMed  Google Scholar 

  • Schäfer H.J., Haag-Kerwer A., Rausch T. 1998. cDNA cloning and expression analysis of genes encloding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial γ-glutamylcysteine synthetase isoform.-Plant Mol. Biol. 37: 87–97.

    PubMed  Google Scholar 

  • Schneider S., Bergmann L. 1995. Regulation of glutathione synthesis in suspension cultures of parsley and tobacco.-Bot. Acta 108: 34–40.

    CAS  Google Scholar 

  • Shi M.M., Kugelman A., Iwamoto T., Tian L., Forman H.J. 1994. Quinone induced oxidative stress elevates glutathione and induces γ-glutamylcysteine synthetase activity in rat lung epithelial L2 cells.-J. Biol. Chem. 42: 26512–26517.

    Google Scholar 

  • Smith I.K., Kendall A.C., Keys A.J., Turner J.C., Lea P.J. 1984. Increased levels of glutathione in a catalase-deficient mutant of barley (Hordeum vulgare L.).-Plant Sci. Lett. 37: 29–33.

    CAS  Google Scholar 

  • Smith I.K. 1985. Stimulation of glutathione synthesis in photorespiring plants by catalse inhibitors.-Plant Physiol. 79: 1044–1047.

    CAS  PubMed  Google Scholar 

  • Smith I.K., Kendall A.C., Keys A.J., Turner J.C., Lea P.J. 1985. The regulation of the biosynthesis of glutathione in leaves of barley (Hordeum vulgare L.).-Plant Sci. 41: 11–17.

    CrossRef  CAS  Google Scholar 

  • Stevens R.G., Creissen G.P., Mullineaux P.M. 1997. Cloning and characterization of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress.-Plant Mol. Biol. 35: 641–654.

    PubMed  CrossRef  CAS  Google Scholar 

  • Strohm M., Jouanin L., Kunert K.-J., Pruvost C., Polle A., Foyer CH., Rennenberg H. 1995. Regulation of glutathione synthesis in leaves of transgenic poplar (Populus tremula x P. alba) overexpressing glutathione synthetase.-Plant J. 7: 141–145.

    CrossRef  CAS  Google Scholar 

  • Sun W.M., Huang Z.Z., Lu S.C. 1996. Regulation of γ-glutamycysteine synthetase by protein phophorylation.-Biochem. J. 320: 321–328.

    PubMed  CAS  Google Scholar 

  • Suthanthiran M., Anderson M.E., Sharma V.K., Meister A. 1990. Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.-Proc. Natl. Acad. Sci. USA 87: 3343–3347.

    PubMed  CAS  Google Scholar 

  • Takahashi H., Chen Z., Du H., Liu Y., Klessig D.F. 1997. Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels.-Plant J. 11: 993–1005.

    PubMed  CrossRef  CAS  Google Scholar 

  • Tate S.S., Meister A. 1985. γ-Glutamyl transpeptidase from kidney.-Meths. Enzymol. 113: 400–419.

    CAS  Google Scholar 

  • Thomas J.A., Poland B., Honzatko R. 1995. Protein sulfhydryls and their role in the antioxidant function of protein S-thiolation.-Arch. Biochem. Biophys. 319: 1–9.

    PubMed  CrossRef  CAS  Google Scholar 

  • Tu Z.H., Anders M.W. 1998. Up-regulation of glutamate-cysteine ligase gene expression by butylated hydroxytoluene is mediated by transcription factor AP-1.-Biochem. Biophys. Res. Comm. 244: 801–805.

    PubMed  CrossRef  CAS  Google Scholar 

  • Ullmann P., Gondet L., Potier S., Bach T.J. 1996. Cloning of Arabidopsis thaliana glutathione synthetase (GSH 2) by functional complementation of a yeast gsh2 mutant.-Eur. J. Biochem. 236: 662–669.

    PubMed  CrossRef  CAS  Google Scholar 

  • Vanacker H., Carver T.L.W., Foyer CH. 2000. Early H 2 O 2 accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barleypowdery mildew interaction.-Plant Physiol. 123: 1289–1300.

    PubMed  CrossRef  CAS  Google Scholar 

  • Vivekanandan M., Edwards G.E. 1987. Activation of NADP-malate dehydrogenase in C 3 plants by reduced glutathione.-Photosynth. Res. 14: 113–124.

    CAS  Google Scholar 

  • Volk S., Feierabend J. 1989. Photoinactivation of catalase at low temperature and its relevance to photosynthetic and peroxide metabolism in leaves.-Plant Cell. Environ. 12: 701–712.

    CAS  Google Scholar 

  • Wild A.C., Gipp J.J., Mulcahy R.T. 1998. Overlapping antioxidant response element and PMA response element sequenes mediate basal and β-napthoflavone-induced expression of the human γ-glutamylcysteine synthetase catalytic subunitgene.-Biochem. J. 332: 373–381.

    PubMed  CAS  Google Scholar 

  • Willekens H., Chamnongpol S., Davey M., Schraudner M., Langebartels C., Van Montagu M., Inzé D., Van Camp W. 1997. Catalase is a sink for H 2 O 2 and is indispensable for stress defence in C 3 plants.-EMBO J. 16: 4806–4816.

    PubMed  CrossRef  CAS  Google Scholar 

  • Wingate V.P.M., Lawton M.A., Lamb C.J. 1988. Glutathione causes a massive and selective induction of plant defense genes.-Plant Physiol. 87: 206–210.

    CAS  CrossRef  PubMed  Google Scholar 

  • Wingsle G., Karpinski S. 1996. Differential redox regulation by glutathione of glutathione reductase and CuZn-superoxide dismutase gene expression in Pinus sylvestris L. needles.-Planta 198: 151–157.

    PubMed  CrossRef  CAS  Google Scholar 

  • Wolf A.E., Dietz K.J., Schroder P. 1996. Degradation of glutathione S-conjugates by a carboxypeptidase in the plant vacuole.-FEBS lett. 384: 31–34.

    PubMed  CrossRef  CAS  Google Scholar 

  • Wolosiuk R.A., Buchanan B.B. 1977. Thioredoxin and glutathione regulate photosynthesis in choroplasts.-Nature 266: 565–567.

    CrossRef  CAS  Google Scholar 

  • Xiang C., Zhong-He M., Lam E. 1996. Coordinated activation of as-1-type elements and a tobacco glutathione-S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.-Plant Mol. Biol. 32: 415–426.

    PubMed  CrossRef  CAS  Google Scholar 

  • Xiang C., Oliver D.J. 1998. Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis.-Plant Cell 10: 1539–1550.

    PubMed  CrossRef  CAS  Google Scholar 

  • Yamaguchi K., Mori H., Nishimura M. 1995. A novel isozyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin.-Plant Cell Physiol. 36: 1157–1162.

    PubMed  CAS  Google Scholar 

  • Zhang L., Robbins M.P., Carver T.L.W., Zeyen R.J. 1997a. Induction of phenylpropanoid gene transcripts in oat attacked by Erysiphe graminis at 20 °C and 10 °C.-Physiol. Mol. Plant Pathol. 51: 15–33.

    CrossRef  CAS  Google Scholar 

  • Zhang H., Wang J., Nickel U., Allen R.D., Goodman H.M. 1997b. Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase.-Plant Mol. Biol. 34: 967–971.

    PubMed  CrossRef  CAS  Google Scholar 

  • Zhu Y., Pilon-Smits E.A.H., Jouanin L., Terry N. 1999. Overexpression of glutathione synthetase in Brassica juncea enhances cadmium tolerance and accumulation.-Plant Physiol. 119: 73–79

    CAS  Google Scholar 

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Foyer, C.H., Noctor, G. (2001). The Molecular Biology and Metabolism of Glutathione. In: Grill, D., Tausz, M., De Kok, L.J. (eds) Significance of Glutathione to Plant Adaptation to the Environment. Plant Ecophysiology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/0-306-47644-4_3

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