Abstract
Nicotianamine (NA) is an intermediate in the biosynthetic pathway of the mugineic acid-family phytosiderophores (MAs), which are crucial components of the iron acquisition of graminaceous (Strategy II) plants. In non-graminaceous (Strategy I ) plants, NA is thought to be an essential chelator for metal cation homeostasis. Nicotianamine synthase (NAS, EC 2.5.1.43) catalyzes the trimerization of S-adenosylmethionine (SAM) to form one molecule of NA. We isolated seven barley cDNA clones (hvnas1- hvnas7), a genomic fragment containing HvNAS1 from barley and three rice cDNA clones (osnas 1- osnas 3), a genomic fragment containing both OsNAS1 and OsNAS2 from rice. We also isolated three Arabidopsis cDNA clones (atnas 1-atnas3). We introduced barley genomic DNA fragments containing HvNAS1 with either 9 or 2 kb of the 5′-flanking region into rice, using Agrobacterium-mediated transformation. Fe deficiency induced HvNAS1 expression in both the roots and leaves of the transgenic rice, as occurs with rice NAS genes.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Higuchi K, Suzuki K, Nakanishi H, Yamaguchi H, Nishizawa NK and Mori S 1999 Plant Physiol. 119, 471–479.
Suzuki K, Higuchi K, Nakanishi H, Nishizawa NK and Mori S 1999 Soil Sci. Plant Nutr. 45, 993–1002.
Higuchi K, Watanabe S, Takahashi M, Kawasaki S, Nakanishi H, Nishizawa NK and Mori S 2001 Plant J. 25, 159–168.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Kluwer Academic Publishers
About this chapter
Cite this chapter
Nishizawa, N.K. et al. (2001). Molecular analysis of nicotianamine synthesis in Strategy I and II plants. In: Horst, W.J., et al. Plant Nutrition. Developments in Plant and Soil Sciences, vol 92. Springer, Dordrecht. https://doi.org/10.1007/0-306-47624-X_23
Download citation
DOI: https://doi.org/10.1007/0-306-47624-X_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-7105-2
Online ISBN: 978-0-306-47624-2
eBook Packages: Springer Book Archive