Abstract
The association/dissociation behaviour in the presence of co-ordinating metal ions and T-R transforming ligands is too complex for rigorous analysis. In the present study equimolar mixtures of insulin labelled with a fluorescence donor and with an acceptor group, respectively, were used to measure fluorescence resonance energy transfer as a function of concentration. These experiments were performed in the presence of 2 Zn or 2Co ions per hexamer and in the absence and presence of KSCN or 3-pentanol, respectively. The results were analysed on the basis of a monomer-dimer-hexamer model in terms of apparent equilibrium constants K 12 and K 26. The experiments were parallelled by measurements of the near-UV circular dichroism on unlabelled insulin and analysed accordingly. Both sets of experiments show that K 26 is not much more increased by Zn than by Co ions and much less than under R 6 conditions.
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Hassiepen, U., Stahl, J., Motzka, B., Federwisch, M. (2002). The Association/Dissociation Equilibria of Insulin in the Presence of Metal Ions: A Fluorescence Energy Transfer and Circular Dichroism Study. In: Dieken, M.L., Federwisch, M., De Meyts, P. (eds) Insulin & Related Proteins - Structure to Function and Pharmacology. Springer, Dordrecht. https://doi.org/10.1007/0-306-47582-0_4
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DOI: https://doi.org/10.1007/0-306-47582-0_4
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