Electrolyzed and Natural Reduced Water Exhibit Insulin-Like Activity on Glucose Uptake into Muscle Cells and Adipocytes

  • M. Oda
  • K. Kusumoto
  • K. Teruya
  • T. Hara
  • T. Maki
  • S. Kabayama
  • Y. Katakura
  • K. Otsubo
  • S. Morisawa
  • H. Hayashi
  • Y. Ishii
  • S. Shirahata

Abstract

In the type 2 diabetes, it has become clear that reactive oxygen species (ROS) cause reduction of glucose uptake by inhibiting the insulin-signaling pathway in muscle cells and adipocytes. We demonstrated that electrolyzed-reduced water (ERW) scavenges ROS and protects DNA from oxidative damage1). Here we found that ERW scavenges ROS in insulin-responsive L6 myotubes and mouse3T3/L1 adipocytes. Uptake of 1-deoxy-D- glucose (2-DOG) into both L6 cells and 3T3/L1 cells was stimulated by ERW in the presence or absence of insulin. This insulin-like activity of ERW was mediated by the activation of PI-3 kinase, resulting in stimulation of translocation of glucose transporter GLUT4 from microsome to plasma membrane. These results suggest that ERW may be useful to improve insulin-independent type 2 diabetes.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Shirahata, S. et al. Biochem. Biophys. Res. Commun. 234, 269–274(1997)PubMedCrossRefGoogle Scholar
  2. 2).
    Bashan N. et al. Am J Physiol, 262, 0682–90(1992)Google Scholar
  3. 3).
    Antonio Ceriello el al. Diabetes, 45, 471–477(19%)Google Scholar
  4. 4).
    Muller H et al. Diabetes, 40, 1440–1448(1991)PubMedCrossRefGoogle Scholar
  5. 5).
    Shirahata, S. el al. Animal Cell Technology, 11, in press (1999).Google Scholar
  6. 6).
    D. Elizabeth Estrada et al. Diabetes, 45, 1798–1804(1996)CrossRefGoogle Scholar
  7. 7).
    Student AK et al. J. Biol. Chem.. 255, 4745–50(1980)PubMedGoogle Scholar
  8. 8).
    Sargeant R el al. Am. J. Physiol, 269(Cell Physiol 38), C217–C225(1995)PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • M. Oda
    • 1
  • K. Kusumoto
    • 1
  • K. Teruya
    • 1
  • T. Hara
    • 1
  • T. Maki
    • 1
  • S. Kabayama
    • 1
  • Y. Katakura
    • 2
  • K. Otsubo
    • 2
  • S. Morisawa
    • 2
  • H. Hayashi
    • 3
  • Y. Ishii
    • 4
  • S. Shirahata
    • 1
  1. 1.Graduate School of Genetic Resources TechnologyKyushu UniversityFukuokaJapan
  2. 2.Nihon Trim Co. Ltd.OsakaJapan
  3. 3.Water InstituteTokyoJapan
  4. 4.Hita Aqua Green Co. Ltd.HitaJapan

Personalised recommendations