Skip to main content

Adaptation of Recombinant HEK-293 Cells to Growth in Serum Free Suspension

  • Chapter
Animal Cell Technology: Products from Cells, Cells as Products

Abstract

HEK-293 cells are a popular host for the generation of stable cell lines for recombinant protein production, and particularly cloned membrane receptors which are required continuously for screening of drug targets. The purpose of this work was to adapt cell lines to growth in suspension culture in order to increase efficiency. This objective was achieved with three recombinant HEK-293 cell lines expressing 5HT receptors, which were successfully adapted for robust growth in suspension culture using a new serum-free medium, Ex-Cell 520 (JRH Biosciences). The adaptation process was cell line dependent and the time scale of this process varied between 4 and 8 weeks. Cells were observed to grow as a mixture of single cells and loose aggregates and could be repeatedly passaged in the range of 6 × 104 cells/ml to 1 × 106 cells/ml with a doubling time of between 24 and 35 hours (cell viability of 95%). In summary, the adaptation process resulted in an increased specific cell productivity, and cell growth rates equivalent to standard cultures and all three cell lines have now been successfully scaled to growth in 50L and 100L bioreactors. In addition, the process achieved an estimated 5-fold reduction in consumable costs.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Editor information

A. Bernard B. Griffiths W. Noé F. Wurm

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Kluwer Academic Publishers

About this chapter

Cite this chapter

Mcallister, R., Schofield, C., Pettman, G., Mannix, C. (1999). Adaptation of Recombinant HEK-293 Cells to Growth in Serum Free Suspension. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_79

Download citation

  • DOI: https://doi.org/10.1007/0-306-46875-1_79

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-6075-9

  • Online ISBN: 978-0-306-46875-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics