Abstract
The importance of glutamine as the major apoptotic inductor in batch cultures of an hybridoma cell line has been investigated. The importance of the caspase cascade, as a possible target for apoptosis inhibition, in the signaling of apoptosis in cultures deprived of glutamine has been also studied. The specific peptidic inhibitor of caspases Ac-DEVD-CHO partially inhibited (25%) the apoptotic process while cysteine protease inhibitor Z-VAD-FMK was a much more effective inhibitor of apoptosis (75%). On the other hand, only with the combination of both inhibitors has been possible to make reversible the programed cell death process.
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© 1999 Kluwer Academic Publishers
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Gabernet, C. et al. (1999). Caspases as a Target for Apoptosis Inhibition in Hybridoma Cells in Culture. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_30
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DOI: https://doi.org/10.1007/0-306-46875-1_30
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
eBook Packages: Springer Book Archive