Abstract
Antitumor protein (AP) from a mushroom, induced the morphological changes typical to apoptosis such as nuclear condensation, aneuploidity, and DNA fragmentation at concentrations as low as 5–20 ng/ml to cancer cells. Molecular alterations related to cell cycle, especially G1/S transition were investigated with a human keratinocyte transformed with oncoproteins, E6 and E7 of human pappiloma virus(HPV)-16. AP didn’t alter significantly an oncosuppressor p53 level, but induced hyperphosphorylation of pRb. Time-dependent change of G1 cyclins, cdk2 and cdk4 after addition of AP showed that expression level of cdk inhibitors, INK4 family, and p27KIP1 did not altered, while that of p21WAF1 was downregulated.
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© 2002 Kluwer Academic Publishers
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Kawamura, Y., Manabe, M., Kitta, K. (2002). Antitumor Protein (AP) from a Mushroom Induced Apoptosis to Transformed Human Keratinocyte by Controlling the Status of pRB, c-MYC, Cyclin E-CDK2, and p21WAF1 in the GI/S Transition. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_47
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DOI: https://doi.org/10.1007/0-306-46869-7_47
Publisher Name: Springer, Dordrecht
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