Summary
We constructed an efficient expression cloning system and isolated a novel α2,3- sialyltransferase (ST3Gal IV) and a novel α1,3-fucosyltransferase (Fuc-TVII). Enzymatic properties and expression patterns of ST3Gal IV and Fuc-TVII indicated that these enzymes were most likely to be involved in the biosynthesis of selectin ligands. ST3Gal IV and Fuc-TVII were produced as secretable forms fused with protein A in a large quantity in Namalwa KJM-1 cells or in Sf9 insect cells. These enzymes are useful for synthesis of sLex oligosaccharides and their derivatives, which should have therapeutic value as selectin antagonists. In addition, compounds inhibiting enzymatic activity or expression of these enzymes are expected to have therapeutic potential as anti-inflammatory drugs. These enzymes may be also useful for modifying sugar chains of therapeutic glycoproteins.
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© 1999 Kluwer Academic Publishers
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Sasaki, K., Kurata-Miura, K., Hasegawa, M., Nishi, T. (1999). Cloning and Application of Glycosyl Transferases Involved in the Biosynthesis of Selectin Ligands. In: Kitagawa, Y., Matsuda, T., Iijima, S. (eds) Animal Cell Technology: Basic & Applied Aspects., vol 1. Springer, Dordrecht. https://doi.org/10.1007/0-306-46865-4_5
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DOI: https://doi.org/10.1007/0-306-46865-4_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5451-2
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