Conclusion
This paper has focused upon potential replacements for animal origin medium components which exhibit comparable biological performance in cell culture systems but eliminate concerns regarding introduction of adventitious contaminants. Nutrient media devoid of components derived from animals are commercially available. Similar strategies may be exploited to modify customized formulations to generate protein-free and even biochemically-defined second generation media to support cellular proliferation and biological production.
Practical considerations for development and quality assurance of nutrient media demand comparable emphasis on process controls for media manufacture. Careful maintenance of facilities and equipment, environmental controls and monitoring, design and control of manufacturing processes, and validated equipment sanitization are required to ensure final product integrity.
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© 1999 Kluwer Academic Publishers
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Jayme, D.W., Smith, S.R. (1999). Options to Eliminate Animal-Derived Components of Cell Culture Media. In: Kitagawa, Y., Matsuda, T., Iijima, S. (eds) Animal Cell Technology: Basic & Applied Aspects., vol 1. Springer, Dordrecht. https://doi.org/10.1007/0-306-46865-4_34
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DOI: https://doi.org/10.1007/0-306-46865-4_34
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