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New Developments and New Applications in Animal Cell Technology pp 329–331Cite as

Measurements of Changes in Cell Size Distribution to Monitor Baculovirus Infection of Insect Cells

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Abstract

We have found that the cell size distributions measured by a CASYTM cell counter can be used to follow the infection process of various insect cell lines. After infection, cell size deviates from the typical distribution of exponentially growing cells, shifting to an increased amount of cells withbigger cell diameters. This deviation has proved to be dependent on the MOI as well as time post-infection. A method is proposed to estimate the degree of infection of a population of insect cells based on the cell size distribution (CSD). The potential of using this method for measuring the ratio of infected to non-infected cells is discussed.

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Authors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung mbH, MascheroderWeg 1, D-38124, Braunschweig, Germany

    Ernesto Chico & Volker JÄger

  2. Center of Molecular Immunology, POBox 16040, Habana, 11600, Cuba

    Ernesto Chico

Authors
  1. Ernesto Chico
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  2. Volker JÄger
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    © 1998 Kluwer Academic Publishers

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    Chico, E., JÄger, V. (1998). Measurements of Changes in Cell Size Distribution to Monitor Baculovirus Infection of Insect Cells. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_59

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    • DOI: https://doi.org/10.1007/0-306-46860-3_59

    • Publisher Name: Springer, Dordrecht

    • Print ISBN: 978-0-7923-5016-3

    • Online ISBN: 978-0-306-46860-5

    • eBook Packages: Springer Book Archive