Conclusions
SDS-PAGE/western blotting of protein from supernatant as well as from lysed virus-infected insect cells (IPLB SF21 AE, BTI Tn5Bl-4) revealed the presence of an intracellularly acumulated β-TP species, which was demonstrated to have a molecular weight different to those observed in the supernatant.
Enzymatic digestion with N-Glycosidase F has proved that the difference between the intra-and extracellular protein pattern was due to different glycosylation stages of the recombinant protein.
The results suggest the presence of a bottle neck in the glycosylation pathway of baculovirus-infected insect cells secreting β-trace protein.
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Rodriguez, G., Conradt, H.S., Jäger, V. (1998). Identification of Altered Glycosylation as the Major Difference Between Intracellulary Accumulated and Secreted β-Trace Protein Produced in Baculovirus-Infected Insect Cells. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_27
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DOI: https://doi.org/10.1007/0-306-46860-3_27
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