Conclusions
Type 1 fimbriae and the FimH-adhesins of E. coli differ in adhesive functions: those of a meningitis isolate IHE3034 mediate adhesion to collagens whereas those of an avirulent K-12 strain PC31 do not. Amino acid residue Alanine-62 in the FimH is critical for the collagen binding ability: substitution of Ala62 of IHE3034 FimH into serine abolishes collagen adhrence whereas substitution of Ser62 of PC31 FimH into alanine restores the binding.
Fimbriae also are targets for binding of functionally important host proteins. Type 1 fimbriae of E. coli differ in plasminogen receptor function: those of the strain IHE3034 mediate plasminogen receptor function while those of the strain PC31 do not. The Fim-protein(s)and the critical amino acids involved in plasminogen receptor function remain to be identified.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Soto, G.E., and Hultgren, S.J., 1999, Bacterial adhesins: common themes and variations in architecture and assembly. J. Bacteriol. 181: 1059–1071.
Krogfelt, K.A., Bergmans, H., and Klemm, P., 1990, Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae. Infect. Immun. 58: 1995–1998.
Sokurenko, E.V., Courtney, H.S., Ohman, D.E., Klemm, P., and Hasty, D., 1994, FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes. J. Bacteriol. 176: 748–755.
Westerlund, B., and Korhonen, T.K., 1993, Bacterial proteins binding to the mammalian extracellular matrix. Mol.Microbiol. 9: 687–694.
Tamm, A., Tarkkanen, A.-M., Korhonen, T.K., Kuusela, P., Toivanen, P., and Skurnik, M., 1993, Hydrophobic domains affect the collagen-binding specificity and surface polymerization as well as the virluence potential of the YadA protein of Yersinia enterocolitica. Mol. Microbiol. 10: 995–1011.
Roggenkamp., A., Neuberger; H.-R., Flugel, A., Schmoll, T., and Heeseman, J., 1995, Substitution of two histidine residues in YadA protein of Yersinia enterocolitica abrogates collagen binding, cell adherence and mouse virulence. Mol. Microbiol. 16: 1207–1219.
Korhonen, T.K., Virkola, R., Lähteenmäki, K., Björkman, Y., Kukkonen, M., Raunio, T., Tarkkanen, A.-M., and Westerlund, B., 1992, Penetration of fimbriate enteric bacteria through basement membranes: a hypothesis. FEMS Microbiol. Lett. 100: 307–312.
Lähteenmäki, K., Virkola, R., Pouttu, R., Kuusela, P., Kukkonen, M., and Korhonen, T.K., 1995, Bacterial plasminogen receptors: in vitro evidence for a role in degradation of the mammalian extracellular matrix. Infect. Immun. 63: 3659–3664.
Pouttu, R., Puustinen, T., Virkola, R., Hacker, J., Klemm, P., and Korhonen, T.K., 1999, Amino-acid residue Ala-62 in the FimH fimbrial adhesin is critical for the adhesiveness of meningitis-associated Escherichia coli to collagens. Mol. Microbiol. 31: 1747–1757.
Klemm, P., Jörgensen, B.J., van Die, I., de Ree, H., and Bergmans, H., 1985, The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization. Mol. Gen. Genet. 199: 410–414.
Kukkonen, M., Raunio, T., Virkola, R., Lähteenmäki, K., Mäkelä, P.H., Klemm, P., Clegg, S., and Korhonen, T.K., 1993, Basement membrane carbohydrate as a target for bacterial adhesion: binding of type 1 fimbria of Salmonella enterica and Escherichia coli to laminin. Mol. Microbiol. 7: 229–237.
Toba, T., Virkola, R., Westerlund, B., Björkman, Y., Sillanpää, J., Vartio, T., Kalkkinen, N., and Korhonen, T.K.,1995, A collagen-binding S-layer protein in Lactobacillus crispatus. Appl. Environ. Microbiol. 61: 2467–2471.
Klemm, P., and Christiansen, G., 1987, Three fim genes required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Mol. Gen. Genet. 208:439–445.
Schembri, M.A., Pallesen, L., Connel, H., Hasty, D.L., and Klemm, P., 1996, Linker insertion analysis of the FimH adhesin of type 1 fimbriae in an Escherichia coli fimH-null background. FEMS Microbiol. Lett. 137: 257–263.
Parkkinen, J., and Korhonen, T.K., 1989, Binding of plasminogen to Escherichia coli adhesion proteins. FEBS Lett. 250: 437–440.
Parkkinen, J., Hacker, J., and Korhonen, T.K., 1991, Enhancement of tissue plasminogen activator catalyzed plasminogen activation by Escherichia coli S fimbriae associated with neonatal septicaemia and meningitis. Thromb. Haemost. 65: 483–486.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
Pouttu, R. et al. (2002). Functional Variability of Type 1 Fimbriae of Escherichia coli. In: Emoődy, L., Pál, T., Hacker, J., Blum-Oehler, G. (eds) Genes and Proteins Underlying Microbial Urinary Tract Virulence. Advances in Experimental Medicine and Biology, vol 485. Springer, Boston, MA. https://doi.org/10.1007/0-306-46840-9_8
Download citation
DOI: https://doi.org/10.1007/0-306-46840-9_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46455-3
Online ISBN: 978-0-306-46840-7
eBook Packages: Springer Book Archive