Genetic Characterization of the Uropathogenic E. coli Strain 536 — A Subtractive Hybridization Analysis
The species E. coli comprises non pathogenic variants and other strains which are able to cause infectious diseases. In order to identify DNA fragments which are present in the pathogenic E. coli strain 536 but absent from the non-pathogenic E. coli strain MG1655 we have performed a subtractive hybridization analysis.
We obtained diverse DNA fragments specific for the E. coli strain 536. Sequence data of several cloned subtractive hybridization fragments showed a homology to known virulence genes. Other E. coli strain 536 specific DNA fragments showed no homology to already known DNA or protein sequences and may represent new virulence genes on PAIs.
In conclusion subtractive hybridization is a successful method for the identification of strain specific DNA sequences. It is a useful approach to complement other genomic approaches such as proteome analysis or differential RNA display analysis.
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