Advertisement

Genetic Characterization of the Uropathogenic E. coli Strain 536 — A Subtractive Hybridization Analysis

  • Britta Janke
  • Jörg Hacker
  • Gabriele Blum-Oehler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 485)

Conclusions

The species E. coli comprises non pathogenic variants and other strains which are able to cause infectious diseases. In order to identify DNA fragments which are present in the pathogenic E. coli strain 536 but absent from the non-pathogenic E. coli strain MG1655 we have performed a subtractive hybridization analysis.

We obtained diverse DNA fragments specific for the E. coli strain 536. Sequence data of several cloned subtractive hybridization fragments showed a homology to known virulence genes. Other E. coli strain 536 specific DNA fragments showed no homology to already known DNA or protein sequences and may represent new virulence genes on PAIs.

In conclusion subtractive hybridization is a successful method for the identification of strain specific DNA sequences. It is a useful approach to complement other genomic approaches such as proteome analysis or differential RNA display analysis.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hacker, J., Blum-Oehler, G., Janke, B., Nagy, G., and Goebel, W., Pathogenicity islands of extraintestinal Escherichia coli. In: Pathogenicity islands and other mobile elements (J. Kaper and J. Hacker, eds.). ASM Press 1999. pp. 59–76.Google Scholar
  2. 2.
    Hacker, J., Blum-Oehler, G., Mühldorfer, I., and Tschôpe, H., 1997, Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution. Mol. Microbiol. 23: 1089–1097.CrossRefPubMedGoogle Scholar
  3. 3.
    Diatchenko, L., Lau, Y.F., Campell, A.P., Chenik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, E.D., and Siebert, P.D., 1996, Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93: 6025–6030.CrossRefPubMedGoogle Scholar
  4. 4.
    Akopyants, N.S., Fradkov, A., Diatchenko, L., Hill, J.E., Siebert, P.D., Lukyanov, S.A., Sverdlov, E.D., and Berg, D.E., 1998, PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc. Natl. Acad. Sci. USA 95: 13108–1313.CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Britta Janke
    • 1
  • Jörg Hacker
    • 1
  • Gabriele Blum-Oehler
    • 1
  1. 1.Institut für Molekulare Infektionsbiologie der Universitôt WürzburgWürzburg

Personalised recommendations