Advertisement

Effect of Spontaneous and Induced Mutations on Outer Membrane Proteins and Lipopolysaccharides of Proteus Penneri Strain 357

  • Ildikó Kustos
  • Vilmos Tóth
  • Ferenc Kilár
  • Béla Kocsis
  • Levente Emo//dy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 485)

Conclusions

Capillary electrophoresis was suitable for the comparative analysis of the OMPs of the wild type and mutant variants of P. penneri strain 357. The patterns were reproducible and characteristic for the different genetic types examined. OMP patterns of the two mutants showed significant changes compared to the wild type. Examination of the LPS molecules showed also altered patterns in the mutants (S-R mutation). One part of alterations in OMP profiles might be explained by the specific interaction of OMP and LPS components;the proper trimerisation and function of porin proteins requires specific LPS structure8. Beside that the mutants showed neither toxicity in vitro, nor pathogenicity in vivo in the virulence assays. So the electrophoretic characterisation of both OMP and LPS composition can significantly improve the sensitivity of classical methods extensively used to identify and characterise bacterial strains.

Keywords

Capillary Electrophoresis Wild Type Strain Outer Membrane Protein Virulence Assay Porin Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Krajden, S., Fuksa, M., Petrea, C., Crisp, L., and Penner, J.L., 1987, Expanded Clinical Spectrum of Infections Caused by Proteus penneri. J. Clin. Microbiol. 25: 578–579PubMedGoogle Scholar
  2. 2.
    Senior, B.W., 1993, The production of HlyA toxin by Proteus penneri strains, J. Med. Microbiol. 39: 282–289.PubMedCrossRefGoogle Scholar
  3. 3.
    Kustos, I., Kocsis, B., Kerepesi, I., and Kilar, F., 1998, Protein profile characterization of bacterial lysates by capillary electrophoresis, Electrophoresis 19: 2324–2330.PubMedGoogle Scholar
  4. 4.
    Westphal, O., Lüderitz, O., Bister, F., 1952, Über die Extraktion von Bakterien mit Phenol-Wasser, Z. Naturforsch. 7b: 148–155.Google Scholar
  5. 5.
    Tsai, C.-M., and Frasch, C.E., 1982, A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels, Anal. Biochem. 119: 115–119.CrossRefPubMedGoogle Scholar
  6. 6.
    Brown, E.J., Griffin, D.E., Rothman, S.W., and Doctor, B.P., 1982, Purification and biological characterization of Shiga toxin from Shigella dysenteriae 1. Infect. Immun. 36: 996–1005.PubMedGoogle Scholar
  7. 7.
    Emo//dy, L., Vörös, S., and Pál, T., 1982, Alpha-haemolysin, a possible virulence factor in Proteus morganii. FEMS Microbiol. Lett. 13: 329–331.Google Scholar
  8. 8.
    Nurminen, M., Hirvas, L., and Vaara, M., 1997, The outer membrane of lipid A-deficient Escherichia coli mutant LH530 has reduced levels of OmpF and leaks periplasmic enzymes, Microbiology 143: 1533–1537.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ildikó Kustos
    • 1
  • Vilmos Tóth
    • 2
  • Ferenc Kilár
    • 3
  • Béla Kocsis
    • 1
  • Levente Emo//dy
    • 1
  1. 1.Department ofMedical Microbiology and ImmunologyUniversity Medical School of PécsHungary
  2. 2.Department of StomatologyUniversity Medical School of PécsHungary
  3. 3.Central Research LaboratoryUniversity Medical School of PécsHungary

Personalised recommendations