Effect of Spontaneous and Induced Mutations on Outer Membrane Proteins and Lipopolysaccharides of Proteus Penneri Strain 357
Capillary electrophoresis was suitable for the comparative analysis of the OMPs of the wild type and mutant variants of P. penneri strain 357. The patterns were reproducible and characteristic for the different genetic types examined. OMP patterns of the two mutants showed significant changes compared to the wild type. Examination of the LPS molecules showed also altered patterns in the mutants (S-R mutation). One part of alterations in OMP profiles might be explained by the specific interaction of OMP and LPS components;the proper trimerisation and function of porin proteins requires specific LPS structure8. Beside that the mutants showed neither toxicity in vitro, nor pathogenicity in vivo in the virulence assays. So the electrophoretic characterisation of both OMP and LPS composition can significantly improve the sensitivity of classical methods extensively used to identify and characterise bacterial strains.
KeywordsCapillary Electrophoresis Wild Type Strain Outer Membrane Protein Virulence Assay Porin Protein
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