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Structural and Functional Studies of the Fimbrial Adhesin Gene Regulator papB from Uropathogenic Escherichia coli

  • Yan Xia
  • kristina Forsman-Semb
  • Jana Jass
  • Bernt Eric Uhlin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 485)

Conclusions

  1. a)

    Purified PapB protein was shown to recognize a motif including a 9- bp repeat sequence containing T/A triplets at a conserved position. PapB binding was affected by distamycin, and the results were consistent with the possibility that the binding to DNA occurred through minor groove interaction.

     
  2. b)

    The DNA binding saturation results and visualization of DNA-PapB complex by AFM suggested that PapB can bind DNA in an oligomeric fashion.

     
  3. c)

    Mutations altering Arg61 or Cys65 caused deficiency in DNA binding, indicating that these residues are critical for PapB binding to DNA. Alanine substitutions at positions 35–36, 53–56, and 74–76 resulted in mutants that were impaired in oligomerization.

     
  4. d)

    The transcriptional efficiency of all the mutants was clearly reduced as compared with that of wild-type PapB, indicating that both DNA binding and oligomerization are required for PapB as a transcriptional regulator.

     

Keywords

Alanine Substitution Transcriptional Efficiency Strain KS71 Binding Saturation Experiment Pilus Biogenesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Båga, M., Göransson, M., Normark, S., and Uhlin, B.E., 1985, Transcriptional activation of a Pap pilus virulence operon from uropathogenic Escherichia coli. EMBO J 4: 3887–3893.PubMedGoogle Scholar
  2. 2.
    Forsman, K., Göransson, M., and Uhlin, B.E., 1989, Autoregulation and multiple DNA interactions by a transcriptional regulatory protein in E. coli pili biogenesis. EMBO J 8Google Scholar
  3. 3.
    Xia, Y., Forsnman, K., Jass J. and Uhlin, B.E., 1998, Oligomeric interaction of the PapB transcriptional regulator with the upstream activating region of pili adhesin gene promoters in Escherichia coli. Mol. Microbiol. 30: 513–523.CrossRefPubMedGoogle Scholar
  4. 4.
    Xia, Y., and Uhlin, B.E., 1999, Mutational analysis of the PapB transcriptional regulator in Escherichia coli. 274: 19723–19730.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Yan Xia
    • 1
  • kristina Forsman-Semb
    • 2
  • Jana Jass
    • 1
  • Bernt Eric Uhlin
    • 1
  1. 1.Department of MicrobiologyUmeå UniversityUmeåSweden
  2. 2.AstraZeneca, Molecular BiologyMölndalSweden

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