Abstract
During terminal maturation of human blood monocytes into macrophages, a multitude of phenotypic and functional changes occurs: cells increase in size, they enhance their capacity for phagocytosis and tumor cytotoxicity but decrease their ability for T-lymphocytestimulation. The pattern of secreted cytokines is shifted as is the profile of surface antigens. We recently identified carboxypeptidase M (CPM) as a macrophage maturation-associated antigendetected by mAb MAX.1/MAX.11. CPM, a phosphoinositol-linked ectopeptidase, is able to process a multitude of different substrates, among them immunologically important peptides like bradykinin, anaphylatoxins and enkephalins. It was previously shown to be expressed in placenta, lung, and kidney. CPM as detected by MAX.1/11 shows a strong expression on monocyte-derived macrophages in vitro and on macrophages in vivo accompanying T-lymphocyte activation like during allogeneic transplant rejection or allergic alveolitis. hi contrast, its expression is suppressed on macrophages by some types of tumor cells. CPM expression seems to correlate with macrophage cytotoxic functions. However, the biological importance of CPM expression in human macrophages in vivo is difficult to predict. A wide range of biologically active peptides are cleaved by CPM, and the relevance of CPM peptide processing during an immune reaction is only poorly understood. The generation and analysis of CPM-deficient animals might improve our understanding of CPM function. Therefore we cloned a cDNA for the murine homologue of CPM. However, expression of mCPM was undetectable in murine primary macrophages and macrophage cell-lines, suggesting that CPM expression and function is not conserved between human and mouse macrophages.
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References
Akagawa, K.S., Takasuka, N., Nozaki, Y., Komuro, L, Azuma, M., Ueda, M., Naito, M., and Takahashi, K., 1996, Blood 88: 4029–4039.
Andreesen, R., Boyce, N.W., and Atkins, R.C., 1987, Transplant. Proc. 19: 2885–2888.
Andreesen, R., Bross, K.J., Osterholz, J., and Emmerich, F., 1986, Blood 67: 1257–1264.
Andreesen, R., Brugger, W., Kunze, R., Stille, W., and von Briesen, H., 1990b, Res. Virol 141: 217–224.
Andreesen, R., Brugger, W., Scheibenbogen, C., Kreutz, M., Leser, H.G., Rehm, A., and Lohr, G.W., 1990a, J. Leukoc. Biol. 47: 490–497.
Andreesen, R., Brugger, W., Thomssen, C., Rehm, A., Speck, B., and Lohr, G.W., 1989, Blood 74: 2150–2156.
Andreesen, R., Gadd, S., Costabel, U., Leser, H.G., Speth, V., Cesnik, B., and Atkins, R.C., 1988, Cell. Tissue Res. 253: 271–279.
Andreesen, R., Osterholz, J., Bross, K.J., Schulz, A., Luckenbach, G.A., and Lohr, G.W., 1983b, Cancer Res. 43: 5931–5936.
Andreesen, R., Picht, J., and Lohr, G.W., 1983a, J. Immunol. Methods 56: 295–304.
Bhoola, K.D., Figueroa, C.D., and Worthy, K., 1992, Pharmacol Rev. 44: 1–80.
Bockmann, S., and Paegelow, I., 1995, Eur. J. Pharmacol. 291: 159–165.
Brugger, W., Kreutz, M., and Andreesen, R., 1991, 7. Leukoc. Biol. 49: 483–488.
Brugger, W., Reinhardt, D., Galanos, C., and Andreesen, R., 1991, Int. Immunol. 3: 221–227.
Deddish, P.A., Skidgel, R. A., Kriho, V.B., Li, X.Y., Becker, R.P., and Erdos, E.G., 1990, J. Biol. Chem. 265: 15083–15089.
Ember, J.A., Sanderson, S.D., Hugli, T.E., and Morgan, E.L., 1994, Am. J. Pathol. 144: 393–403.
Furth, R. van, 1989, Curr. Top. Pathol. 79: 125–150.
Grassi, F., Dezutter-Dambuyant, C., McIlroy, D., Jacquet, C., Yoneda, K., Imamura, S., Boumsell, L., Schmitt, D., Autran, B., Debre, P., and Hosmalin, A., 1998, J. Leukoc. Biol. 64: 484–493. Kreutz, M., and Andreesen, R., 1990, Blood 76: 2457–2461.
Konur, A., Kreutz, M., Knuchel, R., Krause, S.W., and Andreesen, R., 1996, Int. J. Cancer 66: 645–652.
Konur, A., Kreutz, M., Knuchel, R., Krause, S.W., and Andreesen, R., 1998, Int. J. Cancer 78: 648–653.
Maass, G., Schimdt, W., Berger, M., Schlicher, F., Koszik, F., Schneeberger, A., Stingl, G., Birnstiel, M.L., and Schweighoffer, T., 1995, Proc. Natl. Acad. Sci. USA 92: 5540–5544.
Magnan, J., Paterson, S.J., and Kosterlitz, H.W., 1982, Life Sci. 31: 1359–1361.
Mantovani, A., Bottazzi, B., Colotta, F., Sozzani, S., and Ruco, L., 1992, Immunol. Today 13: 265–270.
Matsumura, Y., Maeda, H., and Kato, H., 1990 Agents Actions 29: 172–180.
Nagae, A., Abe, M., Backer, R.P., Deddish, P.A., Skidgel, R.A., and Erdos, E.G., 1993, Am. J. Respir. Cell. Mol Biol. 9: 221–229.
Nagae, A., Deddisch, P.A., Becker, R.P., Anderson, C.H., Abe, M., Tan, F., Skidgel, R.A., and Erdos, E.G., 1992,7. Neurochem. 59: 2201–2212.
Peters, J.H., Gieseler, R., Thiele, B., and Steinbach, F., 1996, Immunol. Today 17: 273–278.
Radulovic, J., Dimitrijevic, M., Laban, O., Stanojevic, S., Vasiljevic, T., Kovacevic-Javanovic, V., and Markovic, B.M., 1995, Peptides 16: 1209–1213.
Radulovic, J., Mancev, Z., Stanojevic, S., Vasiljevic, T., Kovacevic-Jovanovic, V., and Pesic, G., 1996, J. Neuroimmunol. 65: 155–161.
Rehli, M., Krause, S.W., Kreutz, M., and Andreesen, R., 1995, J. Biol. Chem. 270: 15644–15649.
Roy, S., Sedqi, M., Ramakrishnan, S., Barke, R.A., and Loh, H.H., 1996, Cell. Immunol. 169: 271–277.
Rutherford, M.S., Witsell, A., and Schook, L.B., 1993, J. Leukoc. Biol. 53: 602–618.
Saint-Vis, B. de, Cupillard, L., Pandrau-Garcia, D., Ho, S., Renard, N., Grouard, G., Duvert, V., Thomas, X., Galizzi, J.P., and Banchereau, J., 1995, Blood 86: 1098–1105.
Scheibenbogen, C., and Andreesen, R., 1991, J. Leukoc. Biol. 50: 35–42.
Skidgel, R.A., Davis, R.M., and Tan, F., 1989, J. Biol. Chem. 264: 2236–2241.
Skidgel, R.A., 1988, Trends Pharmacol Sci. 9: 299–304.
Steinmann, R.M., 1991, Annu. Rev. Immunol. 9: 271–296.
Sutherland, R.M., 1988, Science 240: 177–184.
Tan, F., Chan, S.J., Steiner, D.F., Schilling, J.W., and Skidgel, R.A., 1989, J. Biol. Chem. 264: 13165–13170.
Tiffany, C.W., and Burch, R.M., 1989, FEBS Lett. 247: 189–192.
Tsuruta, T., Yamamoto, T., Matsubara, S., Nagasawa, S., Tanase, S., Tanaka, J., Takagi, K., and Kambara, T., 1993, Am. J. Pathol 142: 1848–1857.
Udagawa, N., Takahashi, N., Akatsu, T., Tanaka, H., Sasaki, T., Nishihara, T., Koga, T., Martin, T.J., and Suda, T., 1990, Proc. Natl. Acad. Sci. USA 87: 7260–7264.
Wetsel, R.A., 1995, Curr. Opin. Immunol. 7: 48–53.
Yoshioka, S., Fujiwara, H., Yamada, S., Nakayama, T., Higuchi, T., Inoue, T., Mori, T., and Maeda, M., 1998, Mol. Hum. Reprod. 4: 709–717.
Ziegler-Heitbrock, H.W., Fingerle, G., Strobel, M., Schraut, W., Selter, F., Schutt, C., Passlick, B., and Pforte, A., 1993, Eur. J. Immunol. 23: 2053–2058.
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Rehli, M., Krause, S.W., Andreesen, R. (2002). The Membrane-Bound Ectopeptidase CPM as a Marker Of Macrophage Maturation in vitro And in vivo. In: Langner, J., Ansorge, S. (eds) Cellular Peptidases in Immune Functions and Diseases 2. Advances in Experimental Medicine and Biology, vol 477. Springer, Boston, MA. https://doi.org/10.1007/0-306-46826-3_23
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DOI: https://doi.org/10.1007/0-306-46826-3_23
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