Skip to main content
SpringerLink
Log in
Book cover

Current Views of Fatty Acid Oxidation and Ketogenesis pp 27–42Cite as

Topology of Hepatic Mitochondrial Carnitine Palmitoyltransferase I

  • Chapter

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 466))

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fritz, I.B. & Yue, K.T.N. (1963) Long chain carnitine acyltransferase and the role of acylcarnitine derivatives in the catalytic increase of fatty acid oxidation induced by carnitine. J. Lipid Res. 4, 279–288.

    CAS  PubMed  Google Scholar 

  2. Pande, S.V. (1975) A mitochondrial carnitine acylcarnitine translocase system. Proc. Natt. Acad. Sci. U.S.A. 72, 883–887.

    CAS  Google Scholar 

  3. Bremer, J. (1983) Carnitine-metabolism and function. Physiol. Rev. 63, 1420–1480.

    CAS  PubMed  Google Scholar 

  4. Bieber, L.L. (1988) Carnitine. Annu. Rev. Biochem. 57, 261–283.

    Article  CAS  PubMed  Google Scholar 

  5. Kopec, B. & Fritz, I.B. (1973) Comparison of properties of carnitine palmitoylteansferase I with those of carnitine palmitoyltransferase II, and preparation of antibodies. J. Biol. Chem. 248, 4069–4073.

    CAS  PubMed  Google Scholar 

  6. Hoppel, C.L. & Tomec, R.J. (1972) Carnitine palmitoyltransferase: location of two enzymatic activities in rat liver mitochondria. J. Biol. Chem. 247, 832–841.

    CAS  PubMed  Google Scholar 

  7. Murthy, M.S.R. & Pande, S.V. (1987) Malonyl-CoA binding site and overt carnitine palmitoyltransferase activity reside on the opposite sides of the outer mitochondrial membrane. Proc. Natl. Acad. Sci., U.S.A. 84, 378–382.

    CAS  PubMed  Google Scholar 

  8. McGarry, J.D., Mannaerts, G.P. & Foster, D.W. (1977) A possible role for malonyl-CoA in the regulation of hepatic fatty acid oxidation and ketogenesis. J. Clin. Invest. 60, 265–270.

    CAS  PubMed  Google Scholar 

  9. McGarry, J.D., Leatherman, G.F. & Foster, D.W. (1978) CPTase I: The site of inhibition of fatty acid oxidation by malonyl-CoA. J. Biol. Chem. 253, 4128–4136.

    CAS  PubMed  Google Scholar 

  10. McGarry, J.D. & Foster, D.W. (1980) Regulation of hepatic tatty acid oxidation and ketone production. Annu. Rev. Biochem. 49, 395–420.

    Article  CAS  PubMed  Google Scholar 

  11. Bremer, J. (1981) The effect of fasting on the activity of liver carnitine palmitoyltransferase and its inhibition by malonyl-CoA. Biochim. Biophys. Acta 665, 628–631.

    CAS  PubMed  Google Scholar 

  12. Saggerson, E.D. & Carpenter, C.A. (1981) Effects of fasting, adrealectomy and streptozotocin-diabetes on sensitivity of hepatic carnitine acyltransferase to malonyl-CoA. FEBS Lett. 129, 225–228.

    CAS  PubMed  Google Scholar 

  13. Cook, G.A., Otto, D.A. & Cornell, N.W. (1980) Differential inhibition of ketogenesis by malonyl-CoA in mitochondria from fed and starved rats. Biochem. J. 192, 955–958.

    CAS  PubMed  Google Scholar 

  14. Gamble, M.S. & Cook, G.A. (1985) Alteration of the apparent K of carnitine palmitoyltransferase for malonyl-CoA by the diabetic state and reversal by insulin. J. Biol. Chem. 260, 9516–9619.

    CAS  PubMed  Google Scholar 

  15. Cook, G.A. & Gamble, M.S. (1987) Regulation of carnitine palmitoyltransferase by insulin results in decreased activity and decreased apparent K i values for malonyl-CoA. J. Biol. Chem. 262, 2050–2055.

    CAS  PubMed  Google Scholar 

  16. Kashfi, K., Weakley, L.J. & Cook, G.A. (1988) The Effects of Diabetes on the Carnitine Palmitoyl-transferase of Isolated Hepatic Mitochondrial Outer Membrane. Biochem. Soc. Trans. 16, 1010–1011.

    CAS  Google Scholar 

  17. Cook, G.A. (1984) Differences in the sensitivity of carnitine palmitoyltransferase to inhibition by malonyl-CoA are due to differences in K i values. J. Biol. Chem. 259, 12030–12033.

    CAS  PubMed  Google Scholar 

  18. Norum, K.R., Farstad, M. & Bremer, J. (1966) The submitochondrial distribution of acid:CoA ligase (AMP) and palmityl-CoA:carnitine palmityltransferase in rat liver mitochondria. Biochem. Biophys. Res. Commun. 24, 797–804.

    Article  CAS  Google Scholar 

  19. Kashfi, K. & Cook, G.A. (1991) Malonyl-CoA Inhibits Proteolysis of Carnitine Palmitoyltransferase. Biochem. Biophy. Res. Commun. 176, 600–605.

    Google Scholar 

  20. Kashfi, K. & Cook, G.A. (1992) Proteinase Treatment of Intact Hepatic Mitochondria has Differential Effects on Inhibition of Carnitine Palmitoyltansferase by Different Inhibitors. Biochem. J. 282, 909–914.

    CAS  PubMed  Google Scholar 

  21. Mills, S.E., Foster, D.W. & McGarry, J.D. (1983) Interaction of malonyl-CoA and related compounds with mitochondria from different rat tissues. Biochem. J. 214, 83–91.

    CAS  PubMed  Google Scholar 

  22. Kiorpes, T.G., Hoerr, D., Ho, W. Weaner, L.F., Inman, M.G. & Tutwiler, G.F. (1984) Identification of 2-tetradecylglycidyl coenzyme A as the active form of methyl 2-tetradecyglycidate (methyl paloxirate) and its characterization as an irreversible, active site-directed inhibitor of carnitine palmitoyltransferase A in isolated rat liver mitochondria. J. Biol. Chem. 259, 9750–9755.

    CAS  PubMed  Google Scholar 

  23. Bird, M.I. & Saggerson, E.D. (1984) Binding of malonyl-CoA to isolated mitochondria. Evidence for high-and low-affinity sites in liver and heart and relationship to inhibition of carnitine palmitoyltransferase activity. Biochem. J. 222, 639–647.

    CAS  PubMed  Google Scholar 

  24. Declercq P.E., Venincasa, M.D., Mills, S.E., Foster, D.W. & McGarry, J.D. (1985) interaction of malonyl-coA and 2-tetradecylglycidyl-CoA with mitochondrial carnitine palmitoyltransferase I. J. Biol. Chem. 260, 12516–12522.

    CAS  PubMed  Google Scholar 

  25. Grantham, B.D. & Zammit, V.A. (1986) Binding of [14C]malonyl-CoA to rat liver mitochondria sfter blocking the active site of carnitine palmitoyltransferase I. Biochem. J. 233, 589–593.

    CAS  PubMed  Google Scholar 

  26. Cook, G.A. (1987) The hypoglycemic sulfonylureas glyburide and tolbutamide inhibit fatty acid oxidation by inhibiting carnitine palmitoyltransferase. J. Biol. Chem. 262, 4968–4972.

    CAS  PubMed  Google Scholar 

  27. Stephens, T.W., Higgins, A.J., Cook, G.A. & Harris, R.A. (1985) Two mechanisms produce tissue-specific inhibition of fatty acid oxidation by oxfenicine. Biochem. J. 227, 651–660.

    CAS  PubMed  Google Scholar 

  28. Kashfi, K., Israel, M, Sweatman, T.W., Seshadri, R. & Cook, G.A. (1990) Inhibition of Mitochondrial Carnitine Palmitoyltransferases by Adriamycin and Adriamycin Analogues. Biochem. Pharmacol. 40, 1441–1448.

    Article  CAS  PubMed  Google Scholar 

  29. Chase, J.F.A. & Tubbs, P.K. (1969) Conditions for the self-catalysed inactivation of carnitine acetyl-transferase. A novel form of enzyme inhibition. Biochem. J. 111, 225–235.

    CAS  PubMed  Google Scholar 

  30. Stephens, T.W. & Harris, R.A. (1987) Effect of starvation and diabetes on the sensitivity of carnitine palmitoyltransferase I to inhibition by 4-hydroxyphenylglyoxylate. Biochem. J. 243, 405–412.

    CAS  PubMed  Google Scholar 

  31. Kashfi, K., Mynatt, R.L. & Cook, G.A. (1994) Hepatic Carnitine Palmitoyltransferase-I Has Two Independent Inhibitory Binding Sites for Regulation of Fatty Acid Oxidation. Biochem. Biophys. Acta 1212, 245–252.

    CAS  PubMed  Google Scholar 

  32. Cook, G.A., Mynatt, R.L. & Kashfi, K. (1994) Yonetani-TheorellAnalysis of Hepatic Carnitine Palmitoyltransferase-I Inhibition Indicates Two Distinct Inhibitory Binding Sites. J. Biol. Chem. 269, 8803–8807.

    CAS  PubMed  Google Scholar 

  33. Gandour, R.D., Leung, O., Greway, A.T., Ramsay, R.R., Bhaird, N.N., Fronczek, F.R., Bellard, B.M. & Kumaravel, G. (1993) (+)-Hemipalmitoylcarnitinium strongly inhibits carnitine palmitoyltransferase-I in intact mitochondria. J. Med. Chem. 36, 237–242.

    Article  CAS  PubMed  Google Scholar 

  34. Yonetani, T. & Theorell, Y. (1982) The Yonetani-Theorell graphical method for examining overlapping subsites of enzyme active centers. Methods Enzymol. 87C, 500–509.

    Google Scholar 

  35. Fraser, F., Corstorphine, C.G. & Zammit, V.A. (1997) Topology of carnitine palmitoyltransferase I in the mitochondrial outer membrane. Biochem. J. 323, 711–718.

    CAS  PubMed  Google Scholar 

  36. Johnson, D. & Lardy, H. (1967) Isolation of liver or kidney mitochondria. Methods Enzymol 10, 94–96.

    CAS  Google Scholar 

  37. Parsons, D.F., Williams, G.R. & Chance, B. (1966) Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann. New. York. Acad. Sci. 137, 643–666.

    CAS  Google Scholar 

  38. Layne, E. (1957) Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3, 450–451.

    Google Scholar 

  39. Bergmeyer, H.U., Gawehn, K. & Grossl, M. (1974) Enzymes as biochemical reagents. In: Methods of Enzymatic Analysis (Eds. Bergmeyer, H.U. & Gawehn, K.), vol 1, pp. 425–556, Academic Press, London and New York.

    Google Scholar 

  40. Janski, A.M. & Cornell, N.W. (1980) Subcellular distribution of enzymes determined by rapid digitonin fractionation of isolated hepatocytes. Biochem. J. 186, 423–429.

    CAS  PubMed  Google Scholar 

  41. Dixon, M. (1953) The determination of enzyme inhibitor constants. Biochem. J. 55, 170–171.

    CAS  PubMed  Google Scholar 

  42. Kashfi, K., Cagen, L. & Cook, G.A. (1995) Diabetes and Proteolysis: Effects on Carnitine Palmitoyl-transferase-I and Malonyl-CoA Binding. Lipids 30, 383–388.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physical Sciences, Kingsborough Community College, The City University of New York, 2001 Oriental Boulevard, Brooklyn, NY, 11235, USA

    Dr. Khosrow Kashfi

  2. Laboratory of Clinical Microbiology and Immunology, The Rockefeller University, New York, NY

    Dr. Khosrow Kashfi

  3. Department of Pharmacology College of Medicine, University of Tennessee, Memphis, TN

    George A. Cook

Authors
  1. Dr. Khosrow Kashfi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. George A. Cook
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Unit of Paediatric Surgery Institute of Child Health & Great Ormond Street Hospital for Children NHS Trust, University College London Medical School, London, UK

    Patti A. Quant  & Simon Eaton  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Kluwer Academic Publishers

About this chapter

Cite this chapter

Kashfi, K., Cook, G.A. (2002). Topology of Hepatic Mitochondrial Carnitine Palmitoyltransferase I. In: Quant, P.A., Eaton, S. (eds) Current Views of Fatty Acid Oxidation and Ketogenesis. Advances in Experimental Medicine and Biology, vol 466. Springer, Boston, MA. https://doi.org/10.1007/0-306-46818-2_3

Download citation

  • DOI: https://doi.org/10.1007/0-306-46818-2_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-46200-9

  • Online ISBN: 978-0-306-46818-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation