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Downstream Processing in Marine Biotechnology

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Marine Biotechnology II

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 97))

Abstract

Downstream processing is one of the most underestimated steps in bioprocesses and this is not only the case in marine biotechnology. However, it is well known, especially in the pharmaceutical industry, that downstreaming is the most expensive and unfortunately the most ineffective part of a bioprocess. Thus, one might assume that new developments are widely described in the literature. Unfortunately this is not the case. Only a few working groups focus on new and more effective procedures to separate products from marine organisms. A major characteristic of marine biotechnology is the wide variety of products. Due to this variety a broad spectrum of separation techniques must be applied. In this chapter we will give an overview of existing general techniques for downstream processing which are suitable for marine bioprocesses, with some examples focussing on special products such as proteins (enzymes), polysaccharides, polyunsaturated fatty acids and other low molecular weight products. The application of a new membrane adsorber is described as well as the use of solvent extraction in marine biotechnology.

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References

  1. Belter PA, Cussler EL, Hu W-S (1988) Bioseparations. Wiley, New York

    Google Scholar 

  2. Hatti-Kaul R, Mattiasson B (2003) Isolation and purifaction of proteins. Marcel Dekker, New York

    Google Scholar 

  3. Asenjo JA (1990) Separation processes in Biotechnology. Marcel Dekker, New York

    Google Scholar 

  4. Goldberg E (1997) Downstream processing. Chapman & Hall, London

    Google Scholar 

  5. Mengerink KJ, Moy GW, Vacquier VD (2002) J Biol Chem 277:943

    Article  PubMed  Google Scholar 

  6. Middelberg APJ (1995) Biotech Adv 13:491

    Article  Google Scholar 

  7. Gray JP, Herwig RP (1996) Appl Environ Microb 62:4049

    Google Scholar 

  8. Kornilova O, Rosell-Mele A (2003) Org Geochem 34:1517

    Article  Google Scholar 

  9. Martinez K, Barcelo D (2001) Anal Bioanal Chem 370:940

    Google Scholar 

  10. Pino V, Ayala JH, Afonso AM, Gonzalez V (2001) Talanta 54:15

    Article  Google Scholar 

  11. Stephanopoulos G (1993) Biotechnology. VCH, New York

    Google Scholar 

  12. Olaizola M (2003) Biomol Eng 20:459

    Article  PubMed  Google Scholar 

  13. Ganeva V, Galutzov B, Teissie J (2003) Anal Biochem 315:77

    Article  PubMed  Google Scholar 

  14. Datar RV, Rosén CG (1993) Cell and cell debris removal: Centrifugation and cross flow filtration. In: Rehm HJ, Reed G (eds) Biotechnology, 2nd edn. VCH, Weinheim, p 472

    Google Scholar 

  15. Lewis MJ (1996) Pressure-activated membrane processes. In: Grandison AS, Lewis MJ (eds) Separation processes in the food and biotechnology industries. Woodhead, Cambridge, p 65

    Google Scholar 

  16. Ulber R, Plate K, Reif OW, Melzner D (2003) Membranes for protein isolation and purification. In: Hatti-Kaul R, Mattiasson B (eds) Isolation and purifaction of proteins. Marcel Dekker, New York, p 191

    Google Scholar 

  17. Lignot B, Lahogue V, Bourseau P (2003) J Biotechnol 103:281

    Article  PubMed  Google Scholar 

  18. Rossignol N, Vandanjon L, Jaouen P, Quemeneur F (1999) Aquacult Eng 20:191

    Article  Google Scholar 

  19. Dai MH, Buesseler KO, Ripple P, Andrews J, Belastock RA, Gustafsson O, Moran SB (1998) Mar Chem 62:117

    Article  Google Scholar 

  20. Buesseler KO, Bauer JE, Chen RF, Eglinton TI, Gustafsson O, Landing W, Mopper K, Moran SB, Santschi PH, VernonClark R, Wells ML (1996) Mar Chem 55:1

    Article  Google Scholar 

  21. Ulber R, Plate K, Demmer W, Buchholz H, Scheper T (2001) Acta Biotechnol 21:27

    Article  Google Scholar 

  22. Ulber R, Arndt M, Muffler K, Laroche M (2003) Enzymes from marine microorganisms. In: Marine biotechnology an overview of leading fields. Ifremer, Nantes, p 9

    Google Scholar 

  23. Sorokin DY (1995) Microbiology 64:295

    Google Scholar 

  24. Sorokin DY, Vedenina IY, Grabovich MY (1999) Microbiology 68:14

    Google Scholar 

  25. Schügerl K (1994) Solvent extraction in biotechnology. Springer, Berlin Heidelberg New York

    Google Scholar 

  26. Schügerl K (2001) Biotechnol Adv 18:581

    Article  Google Scholar 

  27. Gimeno RA, Comas E, Marce RM, Ferre J, Rius FX, Borrull F (2003) Anal Chim Acta 498:47

    Article  Google Scholar 

  28. Flotron V, Houessou J, Bosio A, Delteil C, Bermond A, Camel V (2003) J Chromatogr A 999:175

    Article  PubMed  Google Scholar 

  29. Chung HY, Ma WCJ, Ang PO, Kim JS, Chen F (2003) J Agr Food Chem 51:2619

    Article  Google Scholar 

  30. Uchida R, Tomoda H, Arai M, Omura S (2001) J Antibot 54:882

    Google Scholar 

  31. Montero O, Lubián (2003) Biomol Eng 20:183

    Article  PubMed  Google Scholar 

  32. Gao DH, Okuda R, Lopez-Avila V (2001) J AOAC Int 84:131

    Google Scholar 

  33. Ramsey ED (1998) Analytical supercritical fluid extraction techniques. Kluwer, Dordrecht

    Google Scholar 

  34. Macías-Sánchez MD, Mantell C, Rodríguez M, Martínez de la Ossa E, Lubián L, Montero O (2003) Supercritical fluid extraction of carotenoids from Nannochloropsis gaditana. www.aidic.it/venicehp/vhpwebpapers/141%20Macia-Sanchez.pdf [16.12.2003]

    Google Scholar 

  35. Roy I, Gupta MN (2003) Selectivity in affinity chromatography. In: Hatti-Kaul R, Mattiasson B (eds) Isolation and purification of proteins. Marcel Dekker, New York, p 57

    Google Scholar 

  36. Odde DJ (1997) Affinity adsorption. In: Goldberg E (ed) Handbook of downstream processing. Chapmann & Hall, London, p 70

    Google Scholar 

  37. Berteau O, McCort I, Goasdoue N, Tissot B, Daniel R (2002) Glycobiology 12:273

    Article  PubMed  Google Scholar 

  38. Sampaio AH, Rogers DJ, Barwell CJ, Saker-Sampaio S, Nascimento KS, Nagano CS, Farias WRL (2002) J Appl Phycol 14:489

    Article  Google Scholar 

  39. Benevides NMB, Holanda ML, Melo FR, Pereira MG, Monteiro ACO, Freitas ALP (2001) Bot Mar 44:17

    Article  Google Scholar 

  40. Hauksson JB, Andresson OS, Asgeirsson B (2000) Enzyme Microb Technol 27:66

    Article  PubMed  Google Scholar 

  41. Croocker PC, Sako Y, Uchida A (1999) Extremophiles 3:3

    Article  PubMed  Google Scholar 

  42. Bannikova GE, Varlamov VP, Miroshnichenko ML, Bonch-Osmolovskaya EA (1998) Biochem Mol Biol Int 44:363

    PubMed  Google Scholar 

  43. Yamamoto T, Nakashizuka M, Kodama H, Kajihara Y, Terada I (1996) J Biochem 120:104

    PubMed  Google Scholar 

  44. Lorenz B, Batel R, Bachinski N, Muller WEG, Schroder HC (1995) Biochim Biophys Acta 1245:17

    PubMed  Google Scholar 

  45. Mohapatro BR, Bapuji M (1998) J Appl Microbiol 84:393

    Article  Google Scholar 

  46. Mohapatro BR, Bapuji M (1997) Lett Appl Microbiol 25:393

    Google Scholar 

  47. Mohapatro BR, Bapuji M, Banerjee UC (1997) Cytobios 92:165

    PubMed  Google Scholar 

  48. Dhevendaran K, Annie K (1999) Indian J Mar Sci 28:3359

    Google Scholar 

  49. Araki T (1998) Japanese Patent, JP 10 295, 372 [98 295, 372] (Cl. C12N9=24), 10th November

    Google Scholar 

  50. Antranikian G (1998) Biotechnology of extremophiles. Springer, Berlin Heidelberg New York

    Google Scholar 

  51. Turkiewicz M, Kur J, Bialkowska A, Cieslinski H, Kalinowska H, Bielecki S (2003) Biomol Eng 20:317

    Article  PubMed  Google Scholar 

  52. Laroche M, Pukall R, Ulber R (2003) Chem Ing Tech 1–2:146

    Article  Google Scholar 

  53. Ramos F, Wiame JM (1982) Eur J Biochem 123:571

    PubMed  Google Scholar 

  54. El-Awamry ZA, Ragab AM, Ghonamy EA, Ahmed SA (1990) Egypt J Microbiol 25:31

    Google Scholar 

  55. Simon D, Hoshino J, Kröger H (1973) Biochim Biophys Acta 321:361

    PubMed  Google Scholar 

  56. Zinecker H, Andreesen JR, Pich A (1998) J Basic Microbiol 38:147

    Article  PubMed  Google Scholar 

  57. Austin PR, Brine CJ, Castle JE, Zikakis JP (1981) Science 212:749–753

    PubMed  Google Scholar 

  58. Ruiz-Herrera J (1978) The distribution and quantitative importance of chitin in fungi. In: Muzzarelli RAA, Priser J (eds) Proceedings of the first international conference chitin and chitosan. MIT Sea Grant Report 78-7, p 11

    Google Scholar 

  59. Tharanathan RN, Kittur FS (2003) Crit Rev Food Sci 43:61

    Article  Google Scholar 

  60. Tsigos I, Martinou A, Kafetzopoulos D, Bouriotis V (2000) TIBTECH 18:305

    Google Scholar 

  61. Onsoyen E, Skaugrud O (1990) J Chem Technol Biotechnol 49:395

    PubMed  Google Scholar 

  62. Muzzarelli RAA, Weckx M, Fillipini O (1989) Carbohyd Polym 11:293

    Article  Google Scholar 

  63. Deans JR, Dixon BG (1992) Bioabsorbents for wastewater treatment. In: Brine CJ, Sandford PA, Zikakis JP (eds) Advances in chitin and chitosan. Elsevier, New York, p 648

    Google Scholar 

  64. Shu XZ, Zhu KJ, Song WH (2001) Int J Pharm 212:19

    Article  PubMed  Google Scholar 

  65. Baba S, Uraki Y, Miura Y, Tokura S (1989) Controlled release and hydrolysis of prodrug using carboxymethylchitin as a drug carrier. In: Brack GS, Anthonsen T, Sandford P (eds) Chitin and chitosan. Elsevier, New York, p 703

    Google Scholar 

  66. Muzzarelli RAA, Mattioli-Belmonte M, Pugnaloni A, Biagini G (1999) Biochemistry, histology and clinical uses of chitins and chitosans in wound healing. In: Jollès P, Muzzarelli RAA (eds) Chitin and chitinases. Birkhäuser, Basel, p 251

    Google Scholar 

  67. Protan Laboratories (1987) Chitosan for cell immobilization. PL1-004, Redmont, WA

    Google Scholar 

  68. Senstad C, Mattiasson B (1989) Biotech Bioeng 34:387

    Article  Google Scholar 

  69. Sinowiecki J, Al-Khateeb N A (2003) Crit Rev Food Sci 43:145

    Article  Google Scholar 

  70. No HK, Meyers SP, Lee KS (1989) J Agric Food Chem 37:575

    Article  Google Scholar 

  71. No HK, Meyers SP (1997) Preparation of chitin and chitosan. In: Muzzarelli RAA, Peter MG (eds) Chitin handbook. European Chitin Society, p 475

    Google Scholar 

  72. Synowiecki J, Sikorski ZE, Naczk M (1981) Biotechnol Bioeng 23:2211

    Article  Google Scholar 

  73. Roberts G (1997) Chitosan production routes and their role in determining the structure and properties of the product. In: Domard A, Roberts GAF, Varum KM (eds) Advances in chitin science. Jaques Andre, Lyon, p 22

    Google Scholar 

  74. Shahidi F, Synowiecki J (1991) J Agric Food Chem 39:1527

    Article  Google Scholar 

  75. Sandford PA (1989) Chitosan – commercial uses and potential applications. In: Brack GS, Anthonsen T, Sandford P (eds) Chitin and chitosan. Elsevier, New York, p 51

    Google Scholar 

  76. Synowiecki J, Al-Khateeb N (2000) Food Chem 68:147

    Article  Google Scholar 

  77. Roberts GAF (1992) Chitin chemistry. Macmillan, London

    Google Scholar 

  78. Chang KLB, Tsai G, Lee J, Fu WR (1997) Carbohyd Res 303:327

    Article  Google Scholar 

  79. Bough WA, Salter WL, Wu ACM, Perkins BE (1978) Biotech Bioeng 20:1931

    Article  Google Scholar 

  80. Ledung P, Milas M, Rinaudo M, Desbrieres J (1994) Carbohyd Polymers 24:209

    Article  Google Scholar 

  81. Tolaimate A, Desbrieres J, Rhazi M, Alagui A (2003) Polymer 44:7939

    Article  Google Scholar 

  82. Kołodziejska I, Wojtasz-Pajak A, Ogonowska G, Sikorski ZE (2000) Bull Sea Fish Inst 2:15

    Google Scholar 

  83. Gao X, Katsumoto T, Onodera K (1995) J Biochem 117:257

    PubMed  Google Scholar 

  84. Tsigos I, Bouriotis V (1995) J Biol Chem 270:26 286

    Google Scholar 

  85. Martinou A Kafetzopoulos D, Bouriotis V (1995) Carbohydr Res 273:235

    Article  Google Scholar 

  86. Berteau O, Mulloy B (2003) Glycobiology 13:29

    Article  Google Scholar 

  87. Mabeau S, Kloareg B, Joseleau JP (1990) Phytochemistry 29:2441

    Article  Google Scholar 

  88. Honya M, Mori H, Anzai M, Araki Y, Nisizawa K (1999) Hydrobiologia 398=399:411

    Article  Google Scholar 

  89. Ribeiro AC, Vieira RP, Mourao PAS, Mulloy B (1994) Carbohyd Res 255:225

    Article  Google Scholar 

  90. Vilela-Silva AC, Alves AP, Valente AP, Vacquier VD, Mourao PAS (1999) Glycobiology 9:927

    Article  PubMed  Google Scholar 

  91. Bakunina IY, Nedashkovskaya OI, Alekseeva SA, Ivanova EP, Romanenko LA, Gorshkova NM, Isakov VV, Zvyagintseva TN, Mikhailov VV (2002) Microbiology 71:41

    Article  Google Scholar 

  92. Gerbst AG, Ustuzhanina NE, Grachev AA, Khatuntseva EA, Tsvetkov DE, Whitfield DM, Berces A, Nifantiev NE (2001) J Carbhyd Chem 20:821

    Article  Google Scholar 

  93. Gerbst AG, Ustuzhanina NE, Grachev AA, Zlotina NS, Khatuntseva EA, Tsvetkov DE, Shashkov AS, Usov AI, Nifantiev NE (2002) J Carbohyd Chem 21:313

    Article  Google Scholar 

  94. Church FC, Meade JB, Treanor RE, Whinna HC (1989) J Biol Chem 264:3618

    PubMed  Google Scholar 

  95. Nishino T, Aizu Y, Nagumo T (1991) Thromb Res 64:723

    Article  PubMed  Google Scholar 

  96. Colliec S, Fisher AM, Tapon-Bretaudière J, Boisson C, Durant P, Jozefonvicz J (1991) Thromb Res 64:143

    Article  PubMed  Google Scholar 

  97. Sinninger V, Tapon-Bretaudière J, Millieu C, Muller D, Jozefonvicz J, Fischer AM (1992) J Chromatogr 615:215

    Google Scholar 

  98. Mauray S, Sternberg C, Theveniaux J, Millet J, Sinquin C, Tapon-Bretaudière J, Fischer AM (1995) Thromb Haemostasis 74:1280

    PubMed  Google Scholar 

  99. Baba M, Nakajima M, Schols D, Pauwels R, Balzarini J, de Clerq E (1988) Antiviral Res 9:335

    Article  PubMed  Google Scholar 

  100. McClure MO, Moore JP, Blanc DF, Scotting P, Cook GM, Keynes RJ, Weber JN, Davies D, Weiss RA (1992) AIDS Res Hum Retroviruses 8:19

    PubMed  Google Scholar 

  101. Baba M, Snoeck R, Pauwels R, de Clerq E (1988) Antimicrob Agents Ch 32:1742

    Google Scholar 

  102. Garcia-Villalon D, Gil-Fernandez C (1991) Antiviral Res 15:139

    Article  PubMed  Google Scholar 

  103. Hahnenberger R, Jakobson AM (1991) Glycoconj J 8:350

    Article  PubMed  Google Scholar 

  104. Lefebvre R, Lo MC, Suarez SS (1997) Biol Reprod 56:1198

    PubMed  Google Scholar 

  105. Bolwell GP, Callow JA, Callow MW, Evans LV (1979) J Cell Sci 36:19

    PubMed  Google Scholar 

  106. Ahuja KK (1982) Exp Cell Res 140:353

    Article  PubMed  Google Scholar 

  107. Peterson RN, Russel LD, Hunt WP (1984) J Exp Zool 231:137

    Article  PubMed  Google Scholar 

  108. Riov D, Colliec-Jovault S, Pinczon du Sel D, Bosch S, Siavoshian S, Le Bert V, Tomasoni C, Sinquin C, Durand P, Roussakis C (1996) Anticancer Res 16:1213

    PubMed  Google Scholar 

  109. Chevolot L, Foucault A, Chaubet F, Kerarec N, Sinquin C, Fisher AM, Boisson-Vidal C (1999) Carbohydr Res 319:154

    Article  PubMed  Google Scholar 

  110. Bakunina IY, Nedashkovskaya OI, Alekseeva SA, Ivanova EP, Romanenko LA, Gorshkova NM, Isakov VV, Zvyagintseva TN, Mikhailov VV (2001) Microbiology 71:41

    Article  Google Scholar 

  111. Kloareg B (1984) Physiol Veg 22:47

    Google Scholar 

  112. Shanmugam M, Mody KH (2000) Curr Sci India 79:1672

    Google Scholar 

  113. Shanmugam M, Mody KH, Siddhanta AK (2001) Indian J Exp 39:365–370

    Google Scholar 

  114. Terumo Corp (1985) Jpn Kokai Tokkyo Koho JP 6042328 Chem Abstr 102:226032

    Google Scholar 

  115. Vilela-Silva AC, Castro MO, Valente AP, Biermann CH, Mourão PAS (2002) J Biol Chem 277:379

    Article  PubMed  Google Scholar 

  116. Pereira MS, Mulloy B, Mourão PAS (1999) J Biol Chem 274:7656

    Article  PubMed  Google Scholar 

  117. Simopoulos AP (1997) Food Rev Int 13:623

    Google Scholar 

  118. Minnis RC, Haq IU, Jackson PR, Yeo WW, Ramsay LE (1998) J Hum Nutr Diet 11:13

    Article  Google Scholar 

  119. Rambjor GS, Walen AI, Windsor SL, Harris WS (1996) Lipids 31:45

    Google Scholar 

  120. Ackman RG (1999) Lipids 34:125

    PubMed  Google Scholar 

  121. Braden LM, Carroll KK (1986) Lipids 21:285

    PubMed  Google Scholar 

  122. Kolanowski W, Swiderski F, Berger S (1999) Int J Food Sci Nutr 50:39

    Article  PubMed  Google Scholar 

  123. Shahidi F, Wanasundara UN (1998) Trends Food Sci Technol 9:230

    Article  Google Scholar 

  124. Yongmanitchai WC, Ward OP (1989) Process Biochem 24:117

    Google Scholar 

  125. Robles Medina A, Molina Grima E, Giménez Giménez A, Ibáñez Gonzáles MJ (1998) Biotechnol Adv 16:517

    Article  PubMed  Google Scholar 

  126. Chisti Y, Moo-Young M (1986) Enzyme Microb Technol 8:194

    Article  Google Scholar 

  127. Garrido F, Banerjee U, Chisti Y, Moo-Young M (1994) Bioseparation 4:319

    PubMed  Google Scholar 

  128. Chuecas L, Riley JP (1969) J Mar Biol Assoc UK 49:97

    Google Scholar 

  129. Kates M (1988) Separation of lipid mixtures. In: Burdon RH, van Knippenberg PH (eds) Techniques of lipidology: isolation, analysis and identification of lipids. Elsevier, Amsterdam, p 186

    Google Scholar 

  130. Bligh EG, Dyer WJ (1959) Can J Biochem Physiol 37:911

    PubMed  Google Scholar 

  131. Nagle N, Lemke P (1990) Appl Biochem Biotechnol 24=25:355

    Google Scholar 

  132. Ahlgren G, Merino L (1991) Arch Hydrobiol 121:295

    Google Scholar 

  133. Molina Grima E, Robles Medina A, Giménez Giménez A, Sánchez Pérez JA, García Camacho F, García Sánchez JL (1994) J Am Oil Chem Soc 71:955

    Google Scholar 

  134. Molina Grima E, Robles Medina A, Giménez Giménez A, Ibáñez González MJ (1996) J Appl Phycol 8:359

    Article  Google Scholar 

  135. Haagsma N, van Gent CM, Luten JB, De Jong RW, van Doorn E (1982) J Am Oil Chem Soc 59:117

    Google Scholar 

  136. Traitler H, Wille HJ, Studer A (1988) J Am Oil Chem Soc 65:755

    Google Scholar 

  137. Wille HJ, Traitler H, Kelley M (1987) Rev Fr Corps Gras 34:69

    Google Scholar 

  138. Medina AR, Giménez AG, Camacho FG, Pérez JAS, Grima EM, Gómez AC (1995) J Am Oil Chem Soc 72:575

    Google Scholar 

  139. Medina AR, Giménez AG, Grima EM, Sánchez JLG (1995) Grasas Aceites 46:174

    Google Scholar 

  140. Cartens M, Molina E, Robles A, Giménez A, Ibáñez MJ (1996) J Am Oil Chem Soc 73:1025

    Google Scholar 

  141. Wang L, Muttucumaru S (2002) Separation of biosynthetic polyunsaturated fatty acid (PUFA) with supercritical fluid. In: El-Gewely (ed) Biotechnology annual review, vol 8. Elsevier, Amsterdam, p 167

    Google Scholar 

  142. Choi KJ, Nakhost Z, Krukonis VJ, Karel M (1987) Food Biotechnol 1:263

    PubMed  Google Scholar 

  143. Polak JT, Balaban M, Peplow A, Philips AJ (1989) Supercritical carbon dioxide extraction of lipids from algae. In: Johnston KP, Penninger JML (eds) Supercritical fluid science and technology. ACS Symposium Series, no 406, p 449

    Google Scholar 

  144. Colwell RR (2002) Biotechnol Adv 20:215

    Article  PubMed  Google Scholar 

  145. Rosenfeld WD, ZoBell CE (1947) J Bacteriol 54:393

    Google Scholar 

  146. Burkholder P, Pfister R, Leitz F (1966) Appl Microbiol 14:649

    PubMed  Google Scholar 

  147. Lovell FM (1966) J Am Chem Soc 88:4510–4511

    Article  Google Scholar 

  148. Faulkner DJ (2000) Anton Leeuw 77:135

    Article  Google Scholar 

  149. Mendola D (2003) Biomolecul Eng 20:441

    Article  Google Scholar 

  150. Proksch P, Edrada RA, Ebel R (2002) Appl Microbiol Biotechnol 59:125

    Article  PubMed  Google Scholar 

  151. Wager-Döbler I, Beil W, Lang S, Meiners M, Laatsch H (2002) Integrated approach to explore the potential of marine microorganisms for the production of bioactive metabolites. In: Scheper T (ed) Advances in biochemical engineering, vol 74. Springer, Berlin Heidelberg New York, p 207

    Google Scholar 

  152. Jensen PR, Kauffman C, Fenical W (1996) Mar Biol 126:1

    Article  Google Scholar 

  153. Newman DJ, Cragg GM, Snader KM (2000) Nat Prod Rep 17:215

    Article  PubMed  Google Scholar 

  154. Haygood MG, Schmidt EW, Davidson SK, Faulkner DJ (1999) J Mol Microbiol Biotechnol 1:33

    PubMed  Google Scholar 

  155. Davidson SK, Allen SW, Lim GE, Anderson CM, Haygood MG (2001) Appl Environ Microbiol 67:4531

    Article  PubMed  Google Scholar 

  156. Riguera R (1997) J Mar Biotechnol 5:187

    Google Scholar 

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  1. Institute of Technical Chemistry, University of Hannover, Callinstr. 3, 30167, Hannover, Germany

    Kai Muffler & Roland Ulber

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Muffler, K., Ulber, R. Downstream Processing in Marine Biotechnology. In: Ulber, R., Le Gal, Y. (eds) Marine Biotechnology II. Advances in Biochemical Engineering/Biotechnology, vol 97. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b135823

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