Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience

Chapter
Part of the Advances in Biochemical Engineering book series (ABE, volume 95)

Abstract

Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience.

TIRF Evanescent Single molecule Nanobioscience Dictyostelium 

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Authors and Affiliations

  1. 1.Laboratory of Nanodevices (endowed by OMRON Co.), Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan
  2. 2.PRESTO, JST and Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan

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