Applications of Thermophiles

  • Jujjavarapu Satya Eswari
  • Swasti Dhagat
  • Ramkrishna Sen


Bioremediation, in environmental biotechnology, is a strategy to control pollution naturally with the help of biological species. The biological species (microorganisms, plants, fungi, or their enzymes) catalyze the biodegradation or biotransformation of toxic chemicals to less toxic forms and hence return the contaminated environment to its original state. EPA describes bioremediation as “treatment that uses naturally occurring organisms to break down hazardous substances into less toxic or non-toxic substances.” The conventional techniques of environment remediation require high input of power and water for degradation of pollutants by thermally enhanced recovery, thermal incineration and desorption, chemical treatment, and in situ flushing of soil by water which further requires treatment of flushed water. The microorganisms cannot degrade inorganic contaminants, and hence not all microorganisms can be used for bioremediation. They can change the valence state of inorganic compounds, precipitate, adsorb, or accumulate the contaminants intracellularly, thereby decreasing the concentration of these inorganic contaminants.


  1. Ahemad M (2012) Implications of bacterial resistance against heavy metals in bioremediation: a review. J Inst Integr Omics Appl Biotechnol (IIOAB) 3(3):39–46Google Scholar
  2. Al-Maghrabi IM et al (1999) Use of thermophilic bacteria for bioremediation of petroleum contaminants. Energy Sources 21(1–2):17–29CrossRefGoogle Scholar
  3. Andrighetto C et al (1998) Molecular identification and cluster analysis of homofermentative thermophilic lactobacilli isolated from dairy products. Res Microbiol 149(9):631–643CrossRefGoogle Scholar
  4. Barnard D et al (2010) Extremophiles in biofuel synthesis. Environ Technol 31(8–9):871–888CrossRefGoogle Scholar
  5. Bentley I Williams E (1996) Starch conversion. Ind Enzymol: 341–357Google Scholar
  6. Cherubini F (2010) The biorefinery concept: using biomass instead of oil for producing energy and chemicals. Energy Convers Manag 51(7):1412–1421CrossRefGoogle Scholar
  7. Chew KW et al (2017) Microalgae biorefinery: high value products perspectives. Bioresour Technol 229:53–62CrossRefGoogle Scholar
  8. Dauphin RD et al (2005) Polyphasic identification of a new thermotolerant species of lactic acid bacteria isolated from chicken faeces. Afr J Biotechnol 4(5):409–421Google Scholar
  9. de Jong E et al (2012) Bio-based chemicals value added products from biorefineries. IEA Bioenergy, Task42 BiorefineryGoogle Scholar
  10. Dhanarajan G et al (2017) Biosurfactant-biopolymer driven microbial enhanced oil recovery (MEOR) and its optimization by an ANN-GA hybrid technique. J Biotechnol 256:46–56CrossRefGoogle Scholar
  11. Du B et al (2006) Expression of a thermostable a-amylase mutant into Escherichia coli and Pichia pastoris. Wei Sheng Wu Xue Bao = Acta Microbiol Sin 46(5):827–830Google Scholar
  12. Eppink M et al (2013) Biorefinery of microalgae: production of high value products, bulk chemicals and biofuels. In: Symposium Biorefinery for Food, Fuel and Materials 2013Google Scholar
  13. Hmidet N et al (2009) Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: characterization and potential application as detergent additive. Biochem Eng J 47(1–3):71–79CrossRefGoogle Scholar
  14. Jacob-Lopes E et al (2015) Microalgal biorefineries. In: Biomass production and uses. IntechOpenGoogle Scholar
  15. Khatiwada D (2013) Assessing the sustainability of bioethanol production in different development contexts: a systems approach. KTH Royal Institute of TechnologyGoogle Scholar
  16. Kitamoto N et al (1988) Cloning and sequencing of the gene encoding thermophilic beta-amylase of Clostridium thermosulfurogenes. J Bacteriol 170(12):5848–5854CrossRefGoogle Scholar
  17. Kosin B, Rakshit SK (2006) Microbial and processing criteria for production of probiotics: a review. Food Technol Biotechnol 44(3):371–379Google Scholar
  18. Kuhad RC et al (2011) Microbial cellulases and their industrial applications. Enzym Res 2011:280696CrossRefGoogle Scholar
  19. Kumar D et al (2008) Microbial proteases and application as laundry detergent additive. Res J Microbiol 3(12):661–672CrossRefGoogle Scholar
  20. Kumar K et al (2015) CO 2 sequestration through algal biomass production. In: Algal biorefinery: an integrated approach. Springer, p 35–57Google Scholar
  21. Ladeira SA et al (2015) Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility. Electron J Biotechnol 18(2):110–115CrossRefGoogle Scholar
  22. Margesin R, Schinner F (2001) Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol 56(5–6):650–663CrossRefGoogle Scholar
  23. Mehta R et al (2016) Insight into thermophiles and their wide-spectrum applications. 3 Biotech 6(1):81CrossRefGoogle Scholar
  24. Meintanis C et al (2006) Biodegradation of crude oil by thermophilic bacteria isolated from a volcano island. Biodegradation 17(2):3–9CrossRefGoogle Scholar
  25. Minhas AK et al (2016) A review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids. Front Microbiol 7:546CrossRefGoogle Scholar
  26. Naik SN et al (2010) Production of first and second generation biofuels: a comprehensive review. Renew Sust Energ Rev 14(2):578–597CrossRefGoogle Scholar
  27. Nanda S et al (2018) A broad introduction to first-, second-, and third-generation biofuels. Recent advancements in biofuels and bioenergy utilization. Springer, pp 1–25Google Scholar
  28. Nascimento WCA d, Martins MLL (2004) Production and properties of an extracellular protease from thermophilic Bacillus sp. Braz J Microbiol 35(1–2):91–96CrossRefGoogle Scholar
  29. Niehaus F et al (1999) Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol 51(6):711–729CrossRefGoogle Scholar
  30. Nisha M, Satyanarayana T (2013) Recombinant bacterial amylopullulanases: developments and perspectives. Bioengineered 4(6):388–400CrossRefGoogle Scholar
  31. Nitisinprasert S et al (2000) Screening and identification of effective thermotolerant lactic acid bacteria producing antimicrobial activity against Escherichia coli and Salmonella sp. resistant to antibiotics. Kasetsart J (Nat Sci) 34:387–400Google Scholar
  32. Ranawat P, Rawat S (2018) Metal-tolerant thermophiles: metals as electron donors and acceptors, toxicity, tolerance and industrial applications. Environ Sci Pollut Res 25(5):4105–4133CrossRefGoogle Scholar
  33. Sar P et al (2013) Metal bioremediation by thermophilic microorganisms. In: Thermophilic microbes in environmental and industrial biotechnology. Springer, pp 171–201Google Scholar
  34. Sarmiento F et al (2015) Cold and hot extremozymes: industrial relevance and current trends. Front Bioeng Biotechnol 3:148CrossRefGoogle Scholar
  35. Shah MP (2014) Environmental bioremediation: a low cost nature’s natural biotechnology for environmental clean-up. J Pet Environ Biotechnol 5(4):1CrossRefGoogle Scholar
  36. Sharma S, Aruna K (2015) Optimization of protease activity of thermophilic Bacillus subtilis WIFD5 isolated from milk powder. Biomed Pharmacol J 5(1):57–64CrossRefGoogle Scholar
  37. Sukumar M et al (2013) Production and partial characterization of extracellular glucose isomerase using thermophilic Bacillus sp. isolated from agricultural land. Biocatal Agric Biotechnol 2(1):45–49CrossRefGoogle Scholar
  38. Svetlitchnyi VA et al (2013) Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria. Biotechnol Biofuels 6(1):31CrossRefGoogle Scholar
  39. Swamy M et al (1994) β-Amylase from Clostridium thermocellum SS8-a thermophilic, anaerobic, cellulolytic bacterium. Lett Appl Microbiol 18(6):301–304CrossRefGoogle Scholar
  40. Vanthoor-Koopmans M et al (2013) Biorefinery of microalgae for food and fuel. Bioresour Technol 135:142–149CrossRefGoogle Scholar
  41. Vijayalakshmi S et al (2011) Screening of alkalophilic thermophilic protease isolated from Bacillus RV. B2. 90 for industrial applications. Res Biotechnol 2(3):32–41Google Scholar
  42. Zhu L (2015) Biorefinery as a promising approach to promote microalgae industry: an innovative framework. Renew Sust Energ Rev 41:1376–1384CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Jujjavarapu Satya Eswari
    • 1
  • Swasti Dhagat
    • 1
  • Ramkrishna Sen
    • 2
  1. 1.Department of BiotechnologyNational Institute of TechnologyRaipurIndia
  2. 2.Department of BiotechnologyIndian Institute of TechnologyKharagpurIndia

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