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Advances in Mass Spectrometry-Based Proteomics and Its Application in Cancer Research

  • Markus HardtEmail author
Chapter

Abstract

With the advent of high-resolution/high mass accuracy instrumentation, sophisticated informatic approaches, and advances in liquid chromatography, mass spectrometry-based proteomics has emerged as an indispensable and widely used tool for the identification, characterization, and quantification of proteins on a large scale. Deep proteome analyses can now sequence over 14,000 protein isoforms for a single human cell line rivaling the depth of next-generation RNA sequencing technology. Without additional enrichment steps, highly sensitive MS-based proteomic studies yield comprehensive identification of major post-translational modifications (PTMs). Isotopic labeling techniques enable the comparison of multiple samples in a single mass spectrometry experiment, while data-independent acquisition strategies provide comprehensive protein coverage and quantification against complex backgrounds.

Keywords

Mass spectrometry Quantitative proteomics Post-translational modifications Cancer pathways Isotopic labeling 

Notes

Acknowledgment

This work was supported by a New Investigator-Idea Development Award (W81XWH-13-1-0250) by the Congressionally Directed Medical Research Program in Prostate Cancer Research.

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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Center for Salivary DiagnosticsThe Forsyth InstituteCambridgeUSA
  2. 2.Harvard School of Dental MedicineBostonUSA

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