Perspectives of Plant Growth-Promoting Rhizobacteria in Conferring Salinity Tolerance in Crops

  • Uttara Oak
  • Amrita Srivastav
  • Vinay Kumar


Soil salinity is imposing serious threats for crop production particularly in arid and semi-arid regions. Various causes for increasing soil salinity in agricultural lands around the globe include weathering of rocks, excessive irrigation, deforestation and poor drainage. Scraping, flushing and leaching are physical means by which soil salinity can be managed, but to a limited extent. Salt-tolerant crop plant varieties are developed by plant biotechnologists to overcome the salinity issues. Bacteria that exist in the rhizoplane and rhizosphere and that are endophytic have shown positive effects on the crop with respect to nutrient availability and therefore are of great importance. The current chapter encompasses the adverse effects of salinity on crop plants and direct and indirect effects of plant growth-promoting rhizobacteria (PGPR) in amelioration of salinity stress and the mechanisms involved thereby. Nitrogen fixation, phosphate solubilisation, phytohormones and the siderophores produced by PGPRs directly make the nutrients available to the plants and allow the crops to grow vigorously. The indirect mechanisms involve production of lytic enzymes, antibiotics that inhibit the pathogen. PGPRs produce osmotolerant chemicals, reactive oxygen species scavenging enzymes and the enzymes that reduce the oxidative stress on the plant system and thereby induce systemic resistance to saline conditions in the plants. In conclusion, the PGPRs can be used as alternate strategy for not just flourishing of the crop plants but also allowing them to withstand a stress condition and thus can be used so that the barren saline lands can be brought under cultivation.


Soil salinity NaCl Plant growth-promoting rhizobacteria (PGPR) Phytohormones Siderophores 



1-aminocyclopropane-1-carboxylate deaminase






Indole-3-acetic acid




Internal PGPR


Indole-3-pyruvic acid


Plant growth-promoting bacteria


Plant growth-promoting rhizobacteria


Phosphate-solubilising bacteria


Reactive oxygen species


Volatile organic compounds



Authors acknowledge the financial support under FIST program of Department of Science and Technology (DST), Government of India, and Star College Scheme of Department of Biotechnology (DBT), Government of India.


  1. Agbodjato NA, Noumavo PA, Baba-Moussa F et al (2015) Characterization of potential plant growth promoting rhizobacteria isolated from maize ( Zea mays L.) in Central and Northern Benin (West Africa). Appl Environ Soil Sci 2015:1–9. Scholar
  2. Ahmad M, Zahir ZA, Asghar HN, Arshad M (2012) The combined application of rhizobial strains and plant growth promoting rhizobacteria improves growth and productivity of mung bean (Vigna radiata L.) under salt-stressed conditions. Ann Microbiol 62:1321–1330. Scholar
  3. Akhtar SS, Andersen MN, Naveed M et al (2015) Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize. Funct Plant Biol 42:770. Scholar
  4. Ali GS, Norman D, El-Sayed AS (2015) Soluble and volatile metabolites of plant growth-promoting Rhizobacteria (PGPRs): role and practical applications in inhibiting pathogens and activating induced systemic resistance (ISR). Adv Bot Res 75:241–284. Scholar
  5. Ashraf M, Hasnain S, Berge O, Mahmood T (2004) Inoculating wheat seedlings with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress. Biol Fertil Soils 40:157–162. Scholar
  6. Beneduzi A, Ambrosini A, Passaglia LMP (2012) Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genet Mol Biol 35:1044–1051CrossRefGoogle Scholar
  7. Berg G (2009) Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl Microbiol Biotechnol 84:11–18. Scholar
  8. Bharti N, Barnawal D (2019) Amelioration of salinity stress by PGPR: ACC deaminase and ROS scavenging enzymes activity. PGPR Amelior Sustain Agric:85–106. Scholar
  9. Bhawsar S (2014) Hydrogen cyanide production in soil bacteria. In: Biotech Artic. Accessed 26 Sept 2018
  10. Chen L, Liu Y, Wu G et al (2016) Induced maize salt tolerance by rhizosphere inoculation of Bacillus amyloliquefaciens SQR9. Physiol Plant 158:34–44. Scholar
  11. Chennappa G, Sreenivasa MY, Nagaraja H (2018) Azotobacter salinestris: a novel pesticide-degrading and prominent biocontrol PGPR bacteria. Springer, Singapore, pp 23–43Google Scholar
  12. de Souza JT, Weller DM, Raaijmakers JM (2003) Frequency, diversity, and activity of 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. in Dutch take-all decline soils. Phytopathology 93:54–63. Scholar
  13. Dobbelaere S, Vanderleyden J, Okon Y (2003) Plant growth-promoting effects of diazotrophs in the rhizosphere. CRC Crit Rev Plant Sci 22:107–149. Scholar
  14. Dong R, Zhang J, Huan H et al (2017) High salt tolerance of a bradyrhizobium strain and its promotion of the growth of Stylosanthes guianensis. Int J Mol Sci 18.
  15. Franche C, Lindström K, Elmerich C (2009) Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant Soil 321:35–59. Scholar
  16. Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications. Scientifica (Cairo) 2012:1–15. Scholar
  17. Glick BR, Cheng Z, Czarny J, Duan J (2007) Promotion of plant growth by ACC deaminase-producing soil bacteria. Eur J Plant Pathol 119:329–339. Scholar
  18. Goswami D, Thakker JN, Dhandhukia PC (2016) Portraying mechanics of plant growth promoting rhizobacteria (PGPR): a review. Cogent Food Agric 2:1127500. Scholar
  19. Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes. Soil Biol Biochem 37:395–412. Scholar
  20. Gururani MA, Upadhyaya CP, Baskar V et al (2013) Plant growth-promoting Rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS-scavenging enzymes and improved photosynthetic performance. J Plant Growth Regul 32:245–258. Scholar
  21. Gutierrez-Manero FJ, Ramos-Solano B, Probanza AN et al (2001) The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiol Plant 111:206–211. Scholar
  22. Habib SH, Kausar H, Saud HM (2016) Plant growth-promoting rhizobacteria enhance salinity stress tolerance in Okra through ROS-scavenging enzymes. Biomed Res Int 2016:1–10. Scholar
  23. Hahm M-S, Son J-S, Hwang Y-J et al (2017) Alleviation of salt stress in pepper (Capsicum annum L.) plants by plant growth-promoting rhizobacteria. J Microbiol Biotechnol 27:1790–1797. Scholar
  24. Hasegawa PM, Bressan RA, Zhu J-K, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499. Scholar
  25. Hayat R, Ali S, Amara U et al (2010) Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol 60:579–598. Scholar
  26. Hossain MM, Das KC, Yesmin S, Shahriar S (2016) Effect of plant growth promoting rhizobacteria (PGPR) in seed germination and root-shoot development of chickpea (Cicer arietinum L.) under different salinity condition. Res Agric Livest Fish 3:105. Scholar
  27. Ilangumaran G, Smith DL (2017) Plant growth promoting rhizobacteria in amelioration of salinity stress: a systems biology perspective. Front Plant Sci 8:1768. Scholar
  28. Jha Y, Subramanian RB (2014) PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiol Mol Biol Plants 20:201–207. Scholar
  29. Jin CW, Ye YQ, Zheng SJ (2014) An underground tale: contribution of microbial activity to plant iron acquisition via ecological processes. Ann Bot 113:7–18. Scholar
  30. Kamei A, Dolai AK, Kamei A (2014) Role of hydrogen cyanide secondary metabolite of plant growth promoting rhizobacteria as biopesticides of weeds. Glob J Sci Front Res D Agric Vet 14:109–112Google Scholar
  31. Kang S-M, Khan AL, Waqas M et al (2014) Plant growth-promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus. J Plant Interact 9:673–682. Scholar
  32. Khare T, Kumar V, Kishor PBK (2015) Na+ and Cl− ions show additive effects under NaCl stress on induction of oxidative stress and the responsive antioxidative defense in rice. Protoplasma 252:1149–1165. Scholar
  33. Khare T, Srivastav A, Shaikh S, Kumar V (2018) Polyamines and their metabolic engineering for plant salinity stress tolerance. In: Salinity responses and tolerance in plants, vol 1. Springer, Cham, pp 339–358CrossRefGoogle Scholar
  34. Kumar V, Khare T (2015) Individual and additive effects of Na+ and Cl ions on rice under salinity stress. Arch Agron Soil Sci 61:381–395. Scholar
  35. Kumar V, Khare T (2016) Differential growth and yield responses of salt-tolerant and susceptible rice cultivars to individual (Na+ and Cl) and additive stress effects of NaCl. Acta Physiol Plant 38:170. Scholar
  36. Kumar V, Khare T (2019) Potent avenues for conferring salinity tolerance in Rice. In: Verma DK, Nadaf AB (eds) Rice science-biotechnological and molecular advancements. Apple Academic Press Inc., USA, pp 29–52. ISBN: 97-8-177-18869-25Google Scholar
  37. Kumar V, Khare T, Sharma M, Wani SH (2017) ROS-induced signaling and gene expression in crops under salinity stress. In: Reactive oxygen species and antioxidant systems in plants: role and regulation under abiotic stress. Springer, Singapore, pp 159–184Google Scholar
  38. Kumar V, Khare T, Shaikh S, Wani SH (2018) Compatible solutes and abiotic stress tolerance in plants. In: Ramakrishna A, Gill SS (eds) Metabolic adaptations in plants during abiotic stress. Taylor & Francis (CRC Press), USA, pp 213–220. ISBN 9781138056381CrossRefGoogle Scholar
  39. Leclère V, Béchet M, Adam A et al (2005) Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism’s antagonistic and biocontrol activities. Appl Environ Microbiol 71:4577–4584. Scholar
  40. Mantri N, Patade V, Penna S, Ford et al (2012) Abiotic stress responses in plants: present and future. In: Abiotic stress responses in plants: metabolism, productivity and sustainability. Springer, New York, New York, NY, pp 1–19Google Scholar
  41. Mayak S, Tirosh T, Glick BR (2004) Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem 42:565–572. Scholar
  42. Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681. Scholar
  43. Parihar P, Singh S, Singh R et al (2015) Effect of salinity stress on plants and its tolerance strategies: a review. Environ Sci Pollut Res 22:4056–4075. Scholar
  44. Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220CrossRefGoogle Scholar
  45. Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environ Microbiol 68:3795–3801. Scholar
  46. Paulucci NS, Gallarato LA, Reguera YB et al (2015) Arachis hypogaea PGPR isolated from Argentine soil modifies its lipids components in response to temperature and salinity. Microbiol Res 173:1–9. Scholar
  47. Pawlowski K, Sirrenberg A (2003) Symbiosis between Frankia and actinorhizal plants: root nodules of non-legumes. Indian J Exp Biol 41:1165–1183PubMedGoogle Scholar
  48. Pessarakli M (1999) Handbook of plant and crop stress. Dekker, BaselGoogle Scholar
  49. Pitman MG, Läuchli A (2002) Global impact of salinity and agricultural ecosystems. In: Salinity: environment – plants – molecules. Kluwer Academic Publishers, Dordrecht, pp 3–20Google Scholar
  50. Pottosin I, Velarde-Buendia AM, Bose J et al (2014) Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses. J Exp Bot 65:1271–1283. Scholar
  51. Qu L, Huang Y, Zhu C et al (2016) Rhizobia-inoculation enhances the soybean’s tolerance to salt stress. Plant Soil 400:209–222. Scholar
  52. Reetha AK, Pavani SL, Mohan S (2014) Hydrogen cyanide production ability by bacterial antagonist and their antibiotics inhibition potential on Macrophomina phaseolina (Tassi.) GoidGoogle Scholar
  53. Rengasamy P (2002) Transient salinity and subsoil constraints to dryland farming in Australian sodic soils: an overview. Aust J Exp Agric 42(3):351–361, CSIRO PublishingCrossRefGoogle Scholar
  54. Rijavec T, Lapanje A (2016) Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate. Front Microbiol 7:1785. Scholar
  55. Santi C, Bogusz D, Franche C (2013) Biological nitrogen fixation in non-legume plants. Ann Bot 111:743–767. Scholar
  56. Sen S, Chandrasekhar CN (2014) Effect of PGPR on growth promotion of rice (Oryza sativa L.) under salt stress. Asian J Plant Sci Res 4:62–67Google Scholar
  57. Shailendra Singh GG, Parihar SS, Ahirwar NK et al (2015) Plant growth promoting rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. J Microb Biochem Technol 07:96–102. Scholar
  58. Shanker A, Venkateswarlu B (2011) Abiotic stress in plants – mechanisms and adaptations. InTechGoogle Scholar
  59. Shrivastava P, Kumar R (2015) Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J Biol Sci 22:123–131. Scholar
  60. Singh JS (2013) Plant growth promoting rhizobacteria. Resonance 18:275–281. Scholar
  61. Smith DL, Subramanian S, Lamont JR, Bywater-Ekegärd M (2015) Signaling in the phytomicrobiome: breadth and potential. Front Plant Sci 6:709. Scholar
  62. Srivastav A, Khare T, Kumar V (2018) Systems biology approach for elucidation of plant responses to salinity stress. In: Salinity responses and tolerance in plants, volume 2. Springer International Publishing, Cham, pp 307–326CrossRefGoogle Scholar
  63. Sujatha NAK (2013) Siderophore production by the isolates of fluorescent pseudomonads. Int J Curr Res Rev 5:01–07Google Scholar
  64. Tank N, Saraf M (2010) Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants. J Plant Interact 5:51–58. Scholar
  65. Wei Y, Zhao Y, Shi M et al (2018) Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilisation during composting with enriched phosphate-solubilizing bacteria inoculation. Bioresour Technol 247:190–199. Scholar
  66. Yang J, Kloepper JW, Ryu C-M (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci 14:1–4. Scholar
  67. Yang Y, Wang N, Guo X et al (2017) Comparative analysis of bacterial community structure in the rhizosphere of maize by high-throughput pyrosequencing. PLoS One 12:e0178425. Scholar
  68. Zheng W, Zeng S, Bais H et al (2018) Plant growth-promoting Rhizobacteria (PGPR) reduce evaporation and increase soil water retention. Water Resour Res 54:3673–3687. Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Uttara Oak
    • 1
  • Amrita Srivastav
    • 1
  • Vinay Kumar
    • 1
  1. 1.Department of Biotechnology, Modern College of Arts, Science and CommerceSavitribai Phule Pune UniversityPuneIndia

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