A Simple Procedure for Isolation, Culture of Protoplast, and Plant Regeneration
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This chapter presents different steps involved in isolation and culture of protoplast; discusses basic steps involved in the developmental sequence from protoplast to plant; and overviews a simple procedure for protoplast isolation, culture, and plant regeneration. Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and other components of cell. Protoplasts are potentially totipotent individuals at the single-cell level. They have the potential to regenerate a cell wall, dedifferentiate, divide mitotically and form unlimited growing cell clones, and differentiate shoot and root meristems or embryos which regenerate whole plant.
KeywordsIsolation Culture Embryos Cell clones Protoplast
- Davey MR, Short KC (1973) The isolation, culture, and fusion of legume and corn flower leaf protoplasts. In: Protoplastes et fusion de cellules somatiques végétales. Colloq Int CNRS, Paris, pp 437–444Google Scholar
- Hess D, Potrykus I, Doon G, Durand T, Hoffmann F (1973) Transformation experiments in higher plants: prerequisites for the use of isolated protoplasts (isolation from mesophyll and callus cultures, uptake of proteins and DNA, and regeneration of whole plants). In: Protoplastes et fusion de cellules somatiques végétales. Colloq Int CNRS, Paris, pp 343–351Google Scholar
- Power JB, Cocking EC (1969) A simple method for the isolation of very large numbers of leaf protoplasts. Biochem J 111:33Google Scholar
- Wallin A, Eriksson T (1972) Plating of protoplasts from cell suspension cultures of Daucus carota. In: Protoplastes et fusion de cellules somatiques végétales. Colloq Int. CNRS, Paris, pp 301–307Google Scholar