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Chapter 15 Sperm Cryopreservation of Aquatic Species

  • Ákos HorváthEmail author
  • Béla Urbányi
Chapter
  • 41 Downloads

Abstract

Cryopreservation of fish sperm is feasible, and methods have been developed for several hundred species. The procedure includes the collection of sperm and assessment of its quality, dilution in suitable extenders, addition of cryoprotectants, loading into freezing devices, cooling, storage, thawing, and finally post-thaw use of sperm. An alternative to traditional freezing methods is the vitrification of sperm which is promising primarily in smaller model fish species. A wide variety of protocols are available in the literature; however, in spite of the significant progress made by the scientific community, commercial application of fish sperm cryopreservation is still very limited.

Keywords

Sperm Quality Freezing Cryopreservation Vitrification 

Notes

Acknowledgments

This research was supported by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent István University as well as the EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union and the European Social Fund. Parts of this chapter are translations from the Hungarian language of the dissertation by Á. Horváth submitted to the Hungarian Academy of Sciences for the title of Doctor of the Hungarisn Academy of Sciences.

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© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Department of AquacultureSzent István UniversityGödöllőHungary

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