Cardiac Cell Specification by Defined Factors
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Our previous study has shown that Tbx5-Gata4-Baf60c-induced functional cardiomyocytes via the ectopic expression of Nkx2-5/Islet1 in the mesodermal cells, but not in the endodermal/ectodermal cells [1, 2] (Fig. 57.1). Mesp1 is one of the major transcriptional regulators specifying the mesodermal lineage, but it also induces skeletal muscle, hematopoietic and vascular cells as well as cardiac cells [3–5]. Eomesodermin (Eomes), an upstream player of Mesp1, regulates mesodermal cell lineages, but it does not have a potential for specification of cardiac cell fate from cardiovascular lineages either . Therefore, the study of cardiac cell fate specification from the embryonic stem cells by the defined factors still remains at least two major questions.
How the cardiac lineage is committed from the mesoderm via repressing skeletal muscle, hematopoietic and vascular cell fates?
Are there any factors specifying the cardiac lineage directly from the undifferentiated stem cells?
Our data just show that a novel gene, Sall1, programs cardiac cell fate and accelerates cardiogenesis, but this factor may act as a defined player for the cardiac cell fate with and the other transcriptional factors to define. Thus, the study for isolation of in vivo Sall1 partners and establishment of its regulation will give us the next window for the program/reprogram research (Fig. 57.1).
- 7.Morita Y, Andersen P, Hotta A, Tsukahara Y, Sasagawa N, Hayashida N, Koga C, Nishikawa M, Saga Y, Evans SM, Koshiba-Takeuchi K, Nishinakamura R, Yoshida Y, Kwon C, Takeuchi JK. Sall1 transiently marks undifferentiated heart precursors and regulates their fate. J Mol Cell Cardiol. 2016;92:158–62.CrossRefGoogle Scholar
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