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Inhibition of Cyclooxygenase Contracts Chicken Ductus Arteriosus

  • Toru Akaike
  • Susumu MinamisawaEmail author
Open Access
Conference paper
  • 484 Downloads

Abstract

Ductus arteriosus (DA) is an essential fetal artery that connects the main pulmonary artery and the descending aorta. Mammalian DA closes right after birth through vasoconstriction via a decrease in circulating prostaglandin E2 (PGE2) [1]. Avian DA also closes after birth although avians have no placenta that is a source of PGE2 in mammals. Previous studies have demonstrated that the PGE2 signal pathway is not involved in the constriction of isolated chicken DA [2]. However, the in vivo effect of PGE2 in avian DA has been little investigated.

Keywords

Ductus arteriosus Prostaglandin Chicken 

Ductus arteriosus (DA) is an essential fetal artery that connects the main pulmonary artery and the descending aorta. Mammalian DA closes right after birth through vasoconstriction via a decrease in circulating prostaglandin E2 (PGE2) [1]. Avian DA also closes after birth although avians have no placenta that is a source of PGE2 in mammals. Previous studies have demonstrated that the PGE2 signal pathway is not involved in the constriction of isolated chicken DA [2]. However, the in vivo effect of PGE2 in avian DA has been little investigated.

We first measured blood concentration of PGE2 in chicken at embryonic day 19 (just before hatching) by enzyme immunoassay to confirm whether PGE2 circulated in the chicken blood during a perinatal period. Plasma PGE2 was significantly high in chicken compared to rat. PGE2 was strongly produced in chicken DA compared to chicken aorta and rat DA. Next, we determined the expression of prostaglandin E receptors (EP1, EP2, EP3 and EP4) in chicken DA. EP2 and EP3 receptors in fetal chicken DA were significantly higher than that of fetal chicken aorta. Especially, the EP2 receptor was significantly up-regulated before hatching and down-regulated after hatching. Finally, we performed a rapid whole-body freezing method to evaluate DA closure in vivo. We measured the internal diameter of DA two hours after in ovo injection of indomethacin, which is a non-selective cyclooxygenase inhibitor. Indomethacin constricted chicken DA at embryonic day 19 but did not constrict chicken aorta. These data suggest that, similar to mammals, PGE2 acts as a vasodilative factor on DA closure in avians.

We demonstrated that factors of the PGE2 signal were detected in chicken DA and inhibition of cyclooxygenase contracted chicken DA. We concluded that the PGE2 signal may play an important role in acute vasodilatation of chicken DA closure.

Notes

Acknowledgment

This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (T.A., S.M.), MEXT-Supported Program for the Strategic Research Foundation at Private Universities (S.M.), the Vehicle Racing Commemorative Foundation (S.M.), The Jikei University Graduate Research Fund (S.M.), and the Miyata Cardiology Research Promotion Foundation (S.M.).

References

  1. 1.
    Yokoyama U, Minamisawa S, Ishikawa Y. Regulation of vascular tone and remodeling of the ductus arteriosus. J Smooth Muscle Res. 2010;46:77–87.CrossRefGoogle Scholar
  2. 2.
    Agren P, van der Sterren S, Cogolludo AL, et al. Developmental changes in the effects of prostaglandin E2 in the chicken ductus arteriosus. J Comp Physiol B. 2009;179(2):133–43.CrossRefGoogle Scholar

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© The Author(s) 2020

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Authors and Affiliations

  1. 1.Department of Cell PhysiologyThe Jikei University School of MedicineMinatoJapan

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