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Detection and Separation of Chito/Chitin Oligosaccharides

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Oligosaccharides of Chitin and Chitosan

Abstract

The bioactivities of chito/chitin oligosaccharides were closely related to their structures, degree of deacetylation (DD) and degree of polymerization (DP). In that case, separation and purification of chito/chitin oligosaccharides is highly beneficial for the further research and application of chito/chitin oligosaccharides, which is essential for the production of high value chito/chitin oligosaccharides in industrial scale. In addition, detection and analysis of chito/chitin oligosaccharides is helps to understand the structure-function relationship more clearly. The chito/chitin oligosaccharides with different degrees of polymerization have different molecular weights, different charge numbers, and different strengths from the separation medium. The separation of chito/chitin oligosaccharides is mainly achieved by combining membrane techniques such as ultrafiltration, nanofiltration and reverse osmosis together to obtain chito oligosaccharides with high purity and narrow interval distribution. For chito/chitin oligosaccharide purification, the most common used methods are absorption chromatography, affinity chromatography, size exclusion chromatography and ion exchange chromatography. For chito/chitin oligosaccharides analysis, TLC, HPLC and electrophoresis were used for qualitative and quantitative analysis of chito/chitin oligosaccharide. MS, IR, NMR and TGA were developed for the structural and sequence analysis according to the sample chemical structure. This chapter mainly reviews recent developments in separation and analysis of chito/chitin oligosaccharides. A variety of separation and analysis methods and examples are discussed.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

    Zhen Qin, Yanying Hou, Waheed Ahamed, Yun Li & Liming Zhao

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  1. Zhen Qin
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  2. Yanying Hou
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  3. Waheed Ahamed
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  4. Yun Li
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  5. Liming Zhao
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Correspondence to Liming Zhao .

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  1. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

    Liming Zhao

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Qin, Z., Hou, Y., Ahamed, W., Li, Y., Zhao, L. (2019). Detection and Separation of Chito/Chitin Oligosaccharides. In: Zhao, L. (eds) Oligosaccharides of Chitin and Chitosan. Springer, Singapore. https://doi.org/10.1007/978-981-13-9402-7_5

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