• Elsayed M. Abdelwhab
  • Ahmed S. Abdel-MoneimEmail author


The viruses under the family Orthomyxoviridae are responsible for a variety of important respiratory diseases in humans and different animal species. The seven genera under the family are the influenza viruses A, B, C and D, Quaranjavirus, Thogotovirus and Isavirus. Viruses are highly evolving, and the genetic reassortment among viruses is seen only within the same genus and never been reported in between viruses from different genera. Influenza A viruses (IAVs) infect humans and different animals including birds, pigs, equines, dogs, cats, whales and seals. To date, there are 18 different haemagglutinins (H1 to H18) and 11 different neuraminidases (N1 to N9) for influenza A viruses. Influenza B viruses (IBVs) are exclusively human pathogens, while influenza C virus (IVC) affects humans and pigs. Serological evidence of ICV was recently detected in camels. Influenza D virus (IDV) was reported in pigs with influenza-like symptoms. IAVs cause recurrent epidemics of varying severity in humans and different animal species due to antigenic drift, gradual accumulation of point mutations, during replication under immune pressure induced by vaccines or prior infections. Several animal species act as important mixing vessel hosts. This chapter provides information on various orthomyxoviruses emphasizing upon virus properties, strains/types, genome, host, ecology, pathobiology, diagnosis and control.


Orthomyxoviruses Avian influenza virus Swine influenza virus Equine influenza virus Infectious salmon anaemia virus Genomics Diagnosis Control Vaccines 


  1. Abdel-Moneim AS, Abdel-Ghany AE, Shany SAS (2010) Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys. J Biomed Sci 17(1):25PubMedPubMedCentralCrossRefGoogle Scholar
  2. Akarsu H, Burmeister WP, Petosa C, Petit I, Müller CW, Ruigrok RWH, Baudin F (2003) Crystal structure of the M1 proteinâ-binding domain of the influenza a virus nuclear export protein (NEP/NS2). EMBO J 22(18):4646–4655PubMedPubMedCentralCrossRefGoogle Scholar
  3. Allison AB, Ballard JR, Tesh RB, Brown JD, Ruder MG, Keel MK, Munk BA, Mickley RM, Gibbs SE, Travassos da Rosa AP, Ellis JC, Ip HS, Shearn-Bochsler VI, Rogers MB, Ghedin E, Holmes EC, Parrish CR, Dwyer C (2015) Cyclic avian mass mortality in the northeastern United States is associated with a novel orthomyxovirus. J Virol 89(2):1389–1403PubMedCrossRefGoogle Scholar
  4. Baskerville A, Lloyd G (1976) The pathogenesis and pathology of experimental Quaranfil virus infection. Br J Exp Pathol 57(2):152–156PubMedPubMedCentralGoogle Scholar
  5. Chen W, Calvo PA, Malide D, Gibbs J, Schubert U, Bacik I, Basta S, O’Neill R, Schickli J, Palese P (2001) A novel influenza a virus mitochondrial protein that induces cell death. Nat Med 7(12):1306PubMedCrossRefPubMedCentralGoogle Scholar
  6. Clifford CM, Thomas LA, Hughes LE, Kohls GM, Philip CB (1968) Identification and comparison of two viruses isolated from ticks of the genus Ornithodoros. Am J Trop Med Hyg 17(6):881–885PubMedCrossRefPubMedCentralGoogle Scholar
  7. Cottet L, Rivas-Aravena A, Cortez-San Martin M, Sandino AM, Spencer E (2011) Infectious salmon anemia virus–genetics and pathogenesis. Virus Res 155(1):10–19PubMedCrossRefGoogle Scholar
  8. Crawford PC, Dubovi EJ, Castleman WL, Stephenson I, Gibbs EPJ, Chen L, Smith C, Hill RC, Ferro P, Pompey J (2005) Transmission of equine influenza virus to dogs. Science 310(5747):482–485PubMedCrossRefGoogle Scholar
  9. Cullinane A, Newton JR (2013) Equine influenza – a global perspective. Vet Microbiol 167(1–2):205–214PubMedCrossRefGoogle Scholar
  10. Cullinane A, Elton D, Mumford J (2010) Equine influenza – surveillance and control. Influenza Other Respir Viruses 4(6):339–344PubMedPubMedCentralCrossRefGoogle Scholar
  11. Daly JM, Lai AC, Binns MM, Chambers TM, Barrandeguy M, Mumford JA (1996) Antigenic and genetic evolution of equine H3N8 influenza A viruses. J Gen Virol 77(4):661–671PubMedCrossRefGoogle Scholar
  12. Daly JM, MacRae S, Newton JR, Wattrang E, Elton DM (2011) Equine influenza: a review of an unpredictable virus. Vet J 189(1):7–14PubMedCrossRefGoogle Scholar
  13. Elton D, Bryant N (2011) Facing the threat of equine influenza. Equine Vet J 43(3):250–258PubMedCrossRefGoogle Scholar
  14. Fodor E, Mingay LJ, Crow M, Deng T, Brownlee GG (2003) A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs. J Virol 77(8):5017–5020PubMedPubMedCentralCrossRefGoogle Scholar
  15. Garner MG, Cowled B, East IJ, Moloney BJ, Kung NY (2011) Evaluating the effectiveness of early vaccination in the control and eradication of equine influenza – a modelling approach. Prev Vet Med 99(1):15–27PubMedCrossRefGoogle Scholar
  16. Garten RJ, Davis CT, Russell CA, Shu B, Lindstrom S, Balish A, Sessions WM, Xu X, Skepner E, Deyde V (2009) Antigenic and genetic characteristics of swine-origin 2009 a (H1N1) influenza viruses circulating in humans. Science 325(5937):197–201PubMedPubMedCentralCrossRefGoogle Scholar
  17. Gaymard A, Le Briand N, Frobert E, Lina B, Escuret V (2016) Functional balance between neuraminidase and haemagglutinin in influenza viruses. Clin Microbiol Infect 22(12):975–983PubMedCrossRefGoogle Scholar
  18. Godoy MG, Aedo A, Kibenge MJT, Groman DB, Yason CV, Grothusen H, Lisperguer A, Calbucura M, Avendaño F, Imilán M (2008) First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile. BMC Vet Res 4(1):28PubMedPubMedCentralCrossRefGoogle Scholar
  19. Gorman OT, Bean WJ, Kawaoka Y, Webster RG (1990a) Evolution of the nucleoprotein gene of influenza a virus. J Virol 64(4):1487–1497PubMedPubMedCentralGoogle Scholar
  20. Gorman OT, Donis RO, Kawaoka Y, Webster RG (1990b) Evolution of influenza a virus PB2 genes: implications for evolution of the ribonucleoprotein complex and origin of human influenza a virus. J Virol 64(10):4893–4902PubMedPubMedCentralGoogle Scholar
  21. Horimoto T, Kawaoka Y (1995) The hemagglutinin cleavability of a virulent avian influenza virus by subtilisin-like endoproteases is influenced by the amino acid immediately downstream of the cleavage site. Virology 210(2):466–470PubMedCrossRefGoogle Scholar
  22. Horvath CM, Williams MA, Lamb RA (1990) Eukaryotic coupled translation of tandem cistrons: identification of the influenza B virus BM2 polypeptide. EMBO J 9(8):2639–2647PubMedPubMedCentralCrossRefGoogle Scholar
  23. Ito T, Gorman OT, Kawaoka Y, Bean WJ, Webster RG (1991) Evolutionary analysis of the influenza a virus M gene with comparison of the M1 and M2 proteins. J Virol 65(10):5491–5498PubMedPubMedCentralGoogle Scholar
  24. Ito T, Couceiro JNSS, Kelm S r, Baum LG, Krauss S, Castrucci MR, Donatelli I, Kida H, Paulson JC, Webster RG (1998) Molecular basis for the generation in pigs of influenza a viruses with pandemic potential. J Virol 72(9):7367–7373PubMedPubMedCentralGoogle Scholar
  25. Janke BH (2014) Influenza a virus infections in swine: pathogenesis and diagnosis. Vet Pathol 51(2):410–426PubMedCrossRefGoogle Scholar
  26. John TJ, Fulginiti VA (1966) Parainfluenza 2 virus: increase in hemagglutinin titer on treatment with Tween-80 and ether. Proc Soc Exp Biol Med 121(1):109–111PubMedCrossRefGoogle Scholar
  27. Kawaoka Y, Krauss S, Webster RG (1989) Avian-to-human transmission of the PB1 gene of influenza a viruses in the 1957 and 1968 pandemics. J Virol 63(11):4603–4608PubMedPubMedCentralGoogle Scholar
  28. Khatri M, Saif YM (2011) Epithelial cells derived from swine bone marrow express stem cell markers and support influenza virus replication in vitro. PLoS One 6(12):e29567PubMedPubMedCentralCrossRefGoogle Scholar
  29. Kibenge FS, Munir K, Kibenge MJ, Joseph T, Moneke E (2004) Infectious salmon anemia virus: causative agent, pathogenesis and immunity. Anim Health Res Rev 5(1):65–78PubMedCrossRefGoogle Scholar
  30. Kobasa D, Kodihalli S, Luo M, Castrucci MR, Donatelli I, Suzuki Y, Suzuki T, Kawaoka Y (1999) Amino acid residues contributing to the substrate specificity of the influenza a virus neuraminidase. J Virol 73(8):6743–6751PubMedPubMedCentralGoogle Scholar
  31. Maclachlan J, Dubovi EJ, Barthold SW, Swayne DE, Winton JR (2017) Orthomyxoviridae. In: Maclachlan J, Dubovi EJ, Barthold SW, Swayne DE, Winton JR (eds) Fenner’s veterinary virology. Elsevier, Amsterdam, pp 389–410Google Scholar
  32. Marc D (2014) Influenza virus non-structural protein NS1: interferon antagonism and beyond. J Gen Virol 95(Pt 12):2594–2611PubMedCrossRefGoogle Scholar
  33. Martin J, Wharton SA, Lin YP, Takemoto DK, Skehel JJ, Wiley DC, Steinhauer DA (1998) Studies of the binding properties of influenza hemagglutinin receptor-site mutants. Virology 241(1):101–111PubMedCrossRefPubMedCentralGoogle Scholar
  34. Matrosovich MN, Matrosovich TY, Gray T, Roberts NA, Klenk H-D (2004) Human and avian influenza viruses target different cell types in cultures of human airway epithelium. Proc Natl Acad Sci USA 101(13):4620–4624PubMedCrossRefPubMedCentralGoogle Scholar
  35. Mochalova L, Gambaryan A, Romanova J, Tuzikov A, Chinarev A, Katinger D, Katinger H, Egorov A, Bovin N (2003) Receptor-binding properties of modern human influenza viruses primarily isolated in Vero and MDCK cells and chicken embryonated eggs. Virology 313(2):473–480PubMedCrossRefPubMedCentralGoogle Scholar
  36. Morens DM, Taubenberger JK (2010) An avian outbreak associated with panzootic equine influenza in 1872: an early example of highly pathogenic avian influenza? Influenza Other Respir Viruses 4(6):373–377PubMedPubMedCentralCrossRefGoogle Scholar
  37. Mumford J (2000) Collaborative study for the establishment of three European pharmacopoeia biological reference preparations for equine influenza horse antiserum. Pharmeuropa 1:7–21Google Scholar
  38. Murcia PR, Wood JLN, Holmes EC (2011) Genome-scale evolution and phylodynamics of equine H3N8 influenza a virus. J Virol 85(11):5312–5322PubMedPubMedCentralCrossRefGoogle Scholar
  39. Nakajima K, Nobusawa E, Ogawa T, Nakajima S (1990) Evolution of the NS genes of the influenza a viruses. I The genetic relatedness of the NS genes of animal influenza viruses. Virus Genes 4(1):5–13PubMedCrossRefPubMedCentralGoogle Scholar
  40. Newton JR, Lakhani KH, Wood JLN, Baker DJ (2000) Risk factors for equine influenza serum antibody titres in young thoroughbred racehorses given an inactivated vaccine. Prev Vet Med 46(2):129–141PubMedCrossRefGoogle Scholar
  41. Newton JR, Daly JM, Spencer L, Mumford JA (2006) Description of the outbreak of equine influenza (H3N8) in the United Kingdom in 2003, during which recently vaccinated horses in Newmarket developed respiratory disease. Vet Rec 158(6):185–192PubMedCrossRefGoogle Scholar
  42. Okazaki K, Kawaoka Y, Webster RG (1989) Evolutionary pathways of the PA genes of influenza a viruses. Virology 172(2):601–608PubMedCrossRefGoogle Scholar
  43. Olsen B, Munster VJ, Wallensten A, Waldenstrom J, Osterhaus AD, Fouchier RA (2006) Global patterns of influenza a virus in wild birds. Science 312(5772):384–388PubMedCrossRefGoogle Scholar
  44. Pensaert M, Ottis K, Vandeputte J, Kaplan MM, Bachmann PA (1981) Evidence for the natural transmission of influenza a virus from wild ducks to swine and its potential importance for man. Bull World Health Organ 59(1):75PubMedPubMedCentralGoogle Scholar
  45. Rahn J, Hoffmann D, Harder TC, Beer M (2015) Vaccines against influenza a viruses in poultry and swine: status and future developments. Vaccine 33(21):2414–2424PubMedCrossRefGoogle Scholar
  46. Rossman JS, Lamb RA (2011) Influenza virus assembly and budding. Virology 411(2):229–236PubMedPubMedCentralCrossRefGoogle Scholar
  47. Salem E, Cook EAJ, Lbacha HA, Oliva J, Awoume F, Aplogan GL, Hymann EC, Muloi D, Deem SL, Alali S, Zouagui Z, Fevre EM, Meyer G, Ducatez MF (2017) Serologic evidence for influenza C and D virus among ruminants and Camelids, Africa, 1991-2015. Emerg Infect Dis 23(9):1556–1559PubMedPubMedCentralCrossRefGoogle Scholar
  48. Shaw M, Cooper L, Xu X, Thompson W, Krauss S, Guan Y, Zhou N, Klimov A, Cox N, Webster R (2002) Molecular changes associated with the transmission of avian influenza a H5N1 and H9N2 viruses to humans. J Med Virol 66(1):107–114PubMedCrossRefGoogle Scholar
  49. Strobl B, Vlasak R (1993) The receptor-destroying enzyme of influenza C virus is required for entry into target cells. Virology 192(2):679–682PubMedCrossRefGoogle Scholar
  50. Stuart-Harris CH, Schild GC, Oxford JS (1985) The epidemiology of influenza. In: Stuart Harris CH, Schild GC, Oxford JS (eds) Influenza: the virus and the disease. Edward Arnold, London, pp 118–130Google Scholar
  51. Su S, Wang L, Fu X, He S, Hong M, Zhou P, Lai A, Gray G, Li S (2014) Equine influenza a (H3N8) virus infection in cats. Emerg Infect Dis 20(12):2096PubMedPubMedCentralCrossRefGoogle Scholar
  52. Su S, Fu X, Li G, Kerlin F, Veit M (2017) Novel influenza D virus: epidemiology, pathology, evolution and biological characteristics. Virulence 8(8):1580–1591PubMedPubMedCentralCrossRefGoogle Scholar
  53. Suarez DL, Sims LD (2013) Influenza. In: Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair VL (eds) Diseases of poultry. Wiley-Blackwell, Hoboken, pp 181–218Google Scholar
  54. Subbarao EK, Kawaoka Y, Ryan-Poirier K, Clements ML, Murphy BR (1992) Comparison of different approaches to measuring influenza a virus-specific hemagglutination inhibition antibodies in the presence of serum inhibitors. J Clin Microbiol 30(4):996–999PubMedPubMedCentralGoogle Scholar
  55. Sutton TC, Finch C, Shao H, Angel M, Chen H, Capua I, Cattoli G, Monne I, Perez DR (2014) Airborne transmission of highly pathogenic H7N1 influenza virus in ferrets. J Virol 88(12):6623–6635PubMedPubMedCentralCrossRefGoogle Scholar
  56. Thorud K, Djupvik HO (1988) Infectious anaemia in Atlantic salmon (Salmo salar L.). Bull Eur Assoc Fish Pathol 8(5):109–111Google Scholar
  57. Torgersen Y (1998) Physical and chemical inactivation of the infectious salmon anaemia (ISA) virus. In: Workshop on ISA. New Brunswick, St Andrews, pp 44–53Google Scholar
  58. Vincent A, Awada L, Brown I, Chen H, Claes F, Dauphin G, Donis R, Culhane M, Hamilton K, Lewis N, Mumford E, Nguyen T, Parchariyanon S, Pasick J, Pavade G, Pereda A, Peiris M, Saito T, Swenson S, Van Reeth K, Webby R, Wong F, Ciacci-Zanella J (2014) Review of influenza a virus in swine worldwide: a call for increased surveillance and research. Zoonoses Public Health 61(1):4–17PubMedCrossRefGoogle Scholar
  59. Webster RG (1993) Are equine 1 influenza viruses still present in horses? Equine Vet J 25(6):537–538PubMedCrossRefGoogle Scholar
  60. Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y (1992) Evolution and ecology of influenza a viruses. Microbiol Rev 56(1):152–179PubMedPubMedCentralGoogle Scholar
  61. Yee KS, Carpenter TE, Cardona CJ (2009) Epidemiology of H5N1 avian influenza. Comp Immunol Microbiol Infect Dis 32(4):325–340PubMedCrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Elsayed M. Abdelwhab
    • 1
  • Ahmed S. Abdel-Moneim
    • 2
    • 3
    Email author
  1. 1.Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal HealthGreifswald-Insel RiemsGermany
  2. 2.Microbiology Department, Virology DivisionCollege of Medicine, Taif UniversityAl-TaifSaudi Arabia
  3. 3.Virology Department, Faculty of Veterinary MedicineBeni-Suef UniversityBeni-SuefEgypt

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