Food Nutrition Immunoassay

  • Chuanlai XuEmail author
  • Hua Kuang
  • Liguang Xu


In recent years, malnutrition, especially vitamin deficiency, is becoming the most common chronic disease due to meal imbalance. Vitamins are essential molecules required for human metabolism such as energy production, immune system, and cell formation. Vitamins deficiency can cause serious health problems, such as neuritis, mouth ulcers, megaloblastic anemia, decreased resistance, as well as osteoporosis. On the other hand, excessive intake of vitamins can also cause poisoning. Therefore, vitamin analysis is critical for disease diagnosis, nutrients assessment, and drug and food production, especially baby food, health food, and functional food. Vitamins immunoassay has caught increasing attention in clinical diagnosis and food detection because of their unique sensitivity and selectivity, excellent timeliness and portability as well as possibility of field analysis. This chapter summarized the immunoassay detection of vitamin B2, biotin, folic acid, and vitamin B12, mainly referring to antigen preparation, antibody characterization, immunoassay establishment, and representative sample analysis. The limitations and challenges of vitamins immunoassay in the rapid and reliable monitoring of food are also considered.


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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.School of Food Science and TechnologyJiangnan UniversityWuxiChina

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