Theranostic Applications of Lysozyme-Based Nanoparticles

  • Sourav Das
  • Manideep Pabba
  • M. E. Dhushyandhun
  • Chitta Ranjan PatraEmail author


Nanotechnology has become an integral part in the domain of therapeutic and diagnostic treatment strategies for various diseases. Amidst various nanoparticles, protein-based nanoparticles have got significant importance owing to their lower toxicity and biodegradability. Among several proteins which are being used in making nanoparticles, lysozyme is one of them. Lysozyme, an anti-microbial enzyme, abundant in secretions of human, serves pivotal role in innate immune system. Recently, scientists have been using this biocompatible protein as template or establishment of framework for developing nanoparticles. Additionally, the readily tunable structures of this stabilized protein also assist in tailoring a broad range of nanoparticles. In this prospect, this chapter will emphasize the overview of recent applications of lysozyme-based nanoparticles in anti-microbial therapy, wound healing, etc., and in diagnostic functions such as cell imaging and sensors. Moreover, the properties of lysozyme as well as its various applications (anti-microbial activity, anti-proliferative activity, amyloid formation ability, etc.) are also narrated in an incisive manner. Additionally, the intricate mechanism underlying the interaction of lysozyme protein with the inorganic metal nanoparticles is also illustrated in this chapter. Herein, this chapter renders the challenges and the possible future direction of the lysozyme-based nanoparticles in theranostic applications.


Lysozyme Nanoparticles Biological activity Amyloid Theranostic applications 



Adenocarcinomic human alveolar basal epithelial cells


2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)


Silver nanoparticles


Lysozyme-gold nanocluster


Circular dichroism


Cystic fibrosis


Critical micelle concentrations


Chronic obstructive pulmonary diseases


Copper nanocluster


Ethylenediaminetetraacetic acid


Epidermal growth factor


Forster non-radiative energy transfer


Fourier-transform infrared spectroscopy


Cervical cancer cells (human)


Horizontal gene transfer


Human lysozyme


High-performance liquid chromatography


Lowest detection limit


Lysozyme microbubble


Matrix-assisted laser desorption/ionization


Michigan cancer foundation-7


Molecular dynamics


Minimum inhibitory concentration








Normal human foreskin fibroblast




Phosphate buffered saline


Pan-drug resistant Acinetobacter baumannii




Recombinant human lysozyme


Reactive oxygen species


Serum amyloid p component


Sodium dodecyl sulfate


Self-assembled nanostructured lysozyme


Transdermal drug delivery










Vancomycin-resistant Enterococcus faecalis


Wild type


X-ray crystallography


2,3-Bis-(2-methoxy-4-nitro5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt



A generous financial support from DST, New Delhi, for “Nanomission Project” (SR/NM/NS-1252/2013; GAP 570) to CRP is duly acknowledged. SD is thankful to UGC, New Delhi, for Senior Research Fellowships. The authors are thankful to the Director, CSIR-IICT for his support and encouragement and for his keen interest in this work. IICT Manuscript No. is IICT/Pubs./2019/021 dated January 28, 2019 for this manuscript is duly acknowledged.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  • Sourav Das
    • 1
    • 2
  • Manideep Pabba
    • 2
  • M. E. Dhushyandhun
    • 2
  • Chitta Ranjan Patra
    • 1
    • 2
    Email author
  1. 1.Academy of Scientific and Innovative Research (AcSIR)GhaziabadIndia
  2. 2.Department of Applied BiologyCSIR-Indian Institute of Chemical TechnologyHyderabadIndia

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