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Molecular Biology Techniques for the Identification and Genotyping of Microorganisms

  • Nisarg Gohil
  • Happy Panchasara
  • Shreya Patel
  • Vijai SinghEmail author
Chapter

Abstract

The advent in molecular biology techniques has enabled, to a great extent, numerous identification and detection techniques of microorganisms by amplifying specific conserved DNA sequences. From time-to-time, the tremendous modifications have been employed in search of a rapid and inexpensive microbial identification method and now a whole genome to be sequenced is possible. In this chapter, we have described the different molecular identification techniques including 16S/18S rRNA, ITS and whole-genome sequencing as well as genotyping techniques such as pulse field gel electrophoresis, AFLP, RAPD, RFLP, ribotyping, BOX, ERIC, rep-PCR and multi-locus sequence typing with their basic principle, procedure, strengths and weaknesses.

Keywords

Identification Genotyping DNA sequencing 16S rRNA 18S rRNA ITS Whole genome sequencing PFGE ERIC BOX Ribotyping AFLP RAPD REP-PCR RFLP MLST 

Notes

Acknowledgments

This work was supported by Puri Foundation for Education in India.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Nisarg Gohil
    • 1
  • Happy Panchasara
    • 1
  • Shreya Patel
    • 1
  • Vijai Singh
    • 1
    • 2
    Email author
  1. 1.School of Biological Sciences and BiotechnologyInstitute of Advanced ResearchGandhinagarIndia
  2. 2.Department of Biosciences, School of SciencesIndrashil UniversityGujaratIndia

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