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Microbial Genome Diversity and Microbial Genome Sequencing

  • Aditi Jangid
  • Tulika PrakashEmail author
Chapter

Abstract

The role of microbiome in agroecosystems has evolved due to improvements in microbial diversity analysis methods. The journey of microbial diversity estimation progressed from culture-dependent to culture-independent methods. The culture-dependent methods are important in finding the microbial diversity of different environments; however, they are immensely biased toward the dominant microorganisms present in a community. With the advancement in sequencing techniques and genomics, the community exploration using culture-independent methods has commenced a new understanding of microbial interactions with their surroundings. Molecular studies of different environmental communities have uncovered <1% of the total number of prokaryotic species representing the cultivable fraction. This chapter summarizes the different methods to acquire a microbial diversity that may eventually enhance plant growth in sustainable agriculture and may often play a role in the management of environmental problems. The merits and demerits of the commonly used molecular methods to investigate microbial communities are discussed. The potential applications of next-generation sequencing techniques for a comprehensive assessment of microbial diversity have been illustrated.

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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.School of Basic SciencesIndian Institute of Technology (IIT)MandiIndia

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