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Plant Viruses as Virus Induced Gene Silencing (VIGS) Vectors

  • Sunny DhirEmail author
  • Ashish Srivastava
  • Nobiyuki Yoshikawa
  • S. M. Paul Khurana
Chapter

Abstract

Virus-induced gene silencing (VIGS) is widely used to analyse the gene functions in model plants and in the plant species where generation of stable genetic transformants to downregulate gene expression is laborious and time-consuming. Plant viruses serve as a suitable candidate for understanding functional genomics by their modification as Virus Induced Gene Silencing vectors. Recent advancements in genetic engineering tools have made a significant contribution to their use as vectors. Here in this chapter, we have tried to discuss about the use of various plant viruses as gene silencing vectors and the next-generation vectors.

Keywords

RNA interference RNA virus RNA silencing CaMV35S 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Sunny Dhir
    • 1
    Email author
  • Ashish Srivastava
    • 1
  • Nobiyuki Yoshikawa
    • 2
  • S. M. Paul Khurana
    • 3
  1. 1.Amity Institute of Virology & ImmunologyAmity UniversityNoidaIndia
  2. 2.Faculty of Agriculture Department of Plant-bioscienceIwate UniversityMoriokaJapan
  3. 3.Amity Institute of BiotechnologyAmity UniversityGurgaonIndia

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