Principles and Implications of Various Genome Enrichment Approaches for Targeted Sequencing of Plant Genomes
The higher eukaryotic organisms harbor huge amounts of DNA in their cells that are generally very complex and dynamic in nature. Strategies of sequencing of higher plants have undergone revolutionary changes concurrent with the development of different and newer chemistries. After the advent of NGS technlogies, the approaches to decipher higher plant genomes have evolved in a precise manner to unlock the genetic potential in a targeted manner. Target enrichment refers to the techniques aiming to reduce genome complexity and enrich for specific subset of the genome for sequencing purposes, to deduce a more meaningful and comprehensive data in a fraction of time, cost and effort. Based on the enrichment mode, these approaches and their modifications are classified as PCR-, hybridization-, Restriction enzyme- based and Enrichment for expressed genomic sequences. Since targeted enrichment techniques confers most of the benefits as those of WGS, these are especially useful for target or trait specific studies and are bound to grow for their diverse applications in both reference and non-model crop species.
KeywordsGenome partitioning Target enrichment approaches Crop improvement Sequencing Plant genomes
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