The Protection of Taurine on Abnormal Expression of Deiodinase 3 Induced by BDE 209 in JEG Cells Under the Normal Culture Conditions
Taurine is an important amino acid for the growth and development of the central nervous system and plays an important role in the development of the nervous system. Many studies have shown that taurine can prevent and repair neurodevelopmental damage, and its mechanism has also become a research hotspot. While most studies focus on nerve cells, less on placental cells that are closely related to early neurodevelopment (developmental neurotoxicity) by modulating fetal circulation level of thyroid hormones. Studies have shown that exposure of placental cells to the common environmental endocrine disruptor BDE 209 during early pregnancy may lead to developmental neurotoxicity due to thyroid hormone interference caused by abnormal expression of deiodinases. Therefore, in this study, the placenta-derived JEG cells cultured at 95% air/5% CO2 was used as a in vitro model, and the potential protection from taurine on BDE 209-mediated cytotoxicity was examined. When BDE 209 was found to cause a decrease in cell viability and disturbance in the gene and protein expressions of placental deiodinase 3, pretreatment of the JEG cells with taurine can moderately reduce the BDE 209-meditated cytotoxicity, and restore gene and protein expressions of placental deiodinase, so that thyroid hormone levels tend to be normal in cell culture medium. Our data suggest that taurine may have some protection on the developmental neurotoxicity caused by BDE 209.
KeywordsJEG cells (Human trophoblastic JEG-3 cells) Polybrominated diphenyl ethers (PBDEs) Taurine Placental deiodinases Developmental neurotoxicity
- BDE 209
- JEG cells
Human trophoblastic JEG-3 cells
This work was supported by the National Natural Science Foundation of China (81773389/H2601 and 81273031/H2601 to JS; Dalian Tech Stars Programme (2016RQ044 to XL) and China’s Post-doctoral Science Fund (2016M591438 to XL).
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