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Taurine 11 pp 923-934 | Cite as

Taurine Promotes Neuritic Growth of Dorsal Root Ganglion Cells Exposed to High Glucose in Vitro

  • Mengren Zhang
  • Inam-u-llah
  • Xiaoxia Shi
  • Pingan Wu
  • Kaixin Li
  • Raheel Suleman
  • Rana Muhammad Aadil
  • Muhammad Zubair Saleem
  • Fengyuan Piao
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Diabetic neuropathy (DN) is the most common chronic complication of DM and its major pathological changes show axonal dysfunction, atrophy and loss. However, there are few reports that taurine promotes neurite growth of dorsal root ganglion (DRG) cells. In current study, DRG neurons were exposed to high glucose (HG) with or without taurine. The neurite outgrowth of DRG neurons was observed by fluorescent immunohistochemistry method. Expression of Gap-43, Akt, phosphorylated Akt, mTOR and phosphorylated mTOR was determined by Western blot assay. Our results showed that HG significantly decreased the neurite outgrowth and expression of Gap-43 in DRG neurons. Moreover, phosphorylated levels of Akt and mTOR were downregulated in DRG neurons exposed to HG. On the contrary, taurine supplementation significantly reversed the decreased neurite outgrowth and Gap-43 expression, and the downregulated phosphorylated levels of Akt and mTOR. However, the protective effects of taurine were blocked in the presence of PI3K antagonists LY294002 or Akt antagonists Perifosine. These results indicate that taurine promotes neurite outgrowth of DRG neurons exposed to HG via activating Akt/mTOR signal pathway.

Keywords

High glucose Diabetic neuropathy Taurine Dorsal root ganglion cells Neurite outgrowth 

Abbreviations

DRG

Dorsal root ganglion

HG

High glucose

T

Taurine

Notes

Conflict of Interest

It is declared that there is no conflict of interest among the authors.

Research Subjects

In this study no animal was harmed or hurd.

Informed Consent

This manuscript is being submitter after consent from all authors and all authors are aware of submission.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Mengren Zhang
    • 1
  • Inam-u-llah
    • 1
  • Xiaoxia Shi
    • 1
  • Pingan Wu
    • 1
  • Kaixin Li
    • 1
  • Raheel Suleman
    • 2
  • Rana Muhammad Aadil
    • 3
  • Muhammad Zubair Saleem
    • 4
  • Fengyuan Piao
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Institute of Food Science and TechnologyGraduate School of Chinese Academy of Agriculture ScienceBeijingChina
  3. 3.National Institute of Food Science and TechnologyUniversity of Agriculture FaisalabadFaisalabadPakistan
  4. 4.Cancer Research CentreDalian Medical UniversityDalianChina

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