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Taurine 11 pp 905-921 | Cite as

Taurine Partially Improves Abnormal Anxiety in Taurine-Deficient Mice

  • Eunkyue ParkEmail author
  • Abdeslem Elidrissi
  • Georgia Schuller-Levis
  • Kathryn K. Chadman
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Taurine is abundant in various tissues including the brain, muscle, heart, spleen, liver and kidney with various physiological functions. Since taurine is produced by cysteine sulfinic acid decarboxylase (CSAD) in the liver and kidney, taurine-deficient mice without CSAD have been investigated for abnormal physiological functions such as retinal development, immune, pancreatic and liver function. In this study, the behavioral effects and abnormal brain development caused by low taurine in the developing brain were examined. In neonatal brains of homozygous CSAD knockout mice (HO), taurine was reduced by 85%, compared to wild-type mice (WT). Taurine was reduced by 35% in the brains of 2 month-old HO, compared to WT. Anxiety, motor coordination and autistic-like behaviors were evaluated at 2 months of age using five behavioral tests: elevated plus maze, open field, social approach, marble burying and accelerating rotarod. Mice were tested from 3 groups including WT, HO and HO with oral treatment of 0.2% taurine in the drinking water (HOT). HOT were born from HO dams treated with taurine from before pregnancy and were continuously treated with taurine in the drinking water after weaning. The taurine levels in the brain and plasma of HOT were restored to WT at 2 months of age. Taurine-deficiency did not lead to changes in autistic-like behaviors as the HO were not significantly different from WT in marble burying and social approach. However, taurine-deficiency increased anxiety-like behavior in HO in the elevated plus maze and open field, compared to WT. Taurine treatment significantly restored the HOT to WT levels of anxiety-like behavior in the elevated plus maze. However, changes in exploratory activity in the open field were not improved with taurine treatment. There was a slight difference in motor ability as the WT mice stayed on the accelerating rotarod longer that the HO and HOT, but the difference was significant in the HOT during the first trial only, compared to WT.

These data support hypothesis that taurine is essential for the emotional development of the brain. First, taurine is remarkably low in the neonatal brain of HO, compared to the adult brain of HO. Second, taurine treatment in HO partially improves anxiety-like behavior to WT.

Keywords

CSAD KO Taurine Behavior Mice Elevated plus maze Open field Social approach Marble burying Rotarod 

Abbreviations

CSAD

cysteine sulfinic acid decarboxylase

CSAD KO

cysteine sulfinic acid decarboxylase knock-out mice

CDO

cysteine dioxygenase

CDO KO

cysteine dioxygenase knock-out mice

ADO

cysteamine (2-aminoethanethiol) dioxygenase

GADL 1

glutamate decarboxylase-like 1

GADL 1 KO

glutamate decarboxylase-like 1 knock-out mice

TauT

taurine transporter

TauT KO

taurine transporter knock-out mice

WT

wild-type mice (CSAD+/+)

HT

heterozygotic mice (CSAD+/−)

HO

homozygotic mice (CSAD−/−)

HOT

homozygotic mice treated with 0.2% taurine in the drinking water

ASD

autistic spectrum disorder

Notes

Acknowledgements

This work was supported by the Office for People with Developmental Disabilities, Albany, NY and Dong A Pharmaceutical Co., LTD, Seoul, Korea. We are thankful to Dr. William Levis and H Cliff Meeker for discussing the research and reviewing this manuscript.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Eunkyue Park
    • 1
    Email author
  • Abdeslem Elidrissi
    • 2
  • Georgia Schuller-Levis
    • 1
  • Kathryn K. Chadman
    • 1
  1. 1.Department of Developmental NeurobiologyNew York Institute for Basic Research in Developmental DisabilitiesStaten IslandUSA
  2. 2.Department of Biological ScienceCollege of Staten IslandStaten IslandUSA

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