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Taurine 11 pp 889-903 | Cite as

Taurine Ameliorates High Glucose Induced Apoptosis in HT-22 Cells

  • Pingan Wu
  • Xiaochi Chen
  • Inam-u-llah
  • Xiaoxia Shi
  • Mengren Zhang
  • Kaixin Li
  • Raheel Suleman
  • Muhammad Shahbaz
  • Shahid Alam
  • Fengyuan Piao
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Diabetes causes memory loss. Hippocampus is responsible for memory and increased apoptosis was found in diabetes patients. Taurine improved memory in diabetes condition. However, mechanism is unclear. In current study, hippocampal cell line HT-22 cells were subjected to analysis as five groups i.e. Control, High glucose (HG) at concentration of 150 mM, HG + 10 mM (T1), 20 mM (T2) and 40 mM (T3) taurine solution. TUNEL assay showed that HG increased the number of apoptotic cell significantly while taurine reduced apoptosis. Taurine increased phosphorylation of Akt in HT-22 cell treated with HG, and increased phosphorylation of Bad (p-Bad) was seen suggesting involvement of Akt/Bad signaling pathway. Expression of Bcl-2 was reduced in HG group but taurine improved this. Bax expression showed opposite trend. This indicated that taurine may reduce apoptosis by controlling balance of Bcl-2 and Bax. When the activation of Akt was blocked by using of perifosine, the effect of taurine disappears either partially or altogether. Thus, it was clear that taurine reduces apoptosis via Akt/Bad pathway in HT-22 cells exposed to HG which further improves downstream balance of Bcl-2 and Bax. This mechanism may be involved in apoptosis of hippocampus cells in diabetic condition.

Keywords

High glucose Apoptosis HT-22 cell Taurine Anti-apoptosis 

Abbreviations

DM

Diabetes mellitus

MTT

4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide

HG

High glucose

TUNEL

In situ TdT-mediated dUTP nick end labeling

Notes

Acknowledgement

This work was supported by National Natural Science Foundation of China (grant numbers 81501574).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Pingan Wu
    • 1
  • Xiaochi Chen
    • 2
  • Inam-u-llah
    • 1
  • Xiaoxia Shi
    • 1
  • Mengren Zhang
    • 1
  • Kaixin Li
    • 1
  • Raheel Suleman
    • 3
  • Muhammad Shahbaz
    • 4
  • Shahid Alam
    • 5
  • Fengyuan Piao
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Department of UrologyThe First Affiliated Hospital of Dalian Medical UniversityDalianChina
  3. 3.Institute of Food Science and TechnologyGraduate School of Chinese Academy of Agriculture ScienceBeijingChina
  4. 4.Department of Food Science and TechnologyMuhammad Nawaz Sharif University of AgricultureMultanPakistan
  5. 5.Department of AnatomyDalian Medical UniversityDalianChina

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