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Taurine 11 pp 857-867 | Cite as

Glucose-Taurine Reduced Exerts Neuroinflammatory Responses by Inhibition of NF-κB Activation in LPS-Induced BV2 Microglia

  • Hwan Lee
  • Dong-Sung Lee
  • Kyung Ja Chang
  • Sung Hoon Kim
  • Sun Hee Cheong
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

We want to find the anti-neuroinflammatory action of the taurine derivative Glucose-Taurine Reduced (G-T-R). The anti-neuroinflammatory action by G-T-R were investigated in lipopolysaccharide (LPS)-induced BV2 microglia. G-T-R inhibited the production of nitric oxide and prostaglandin E2, and down-regulated the protein expression of inducible NO synthase and cyclooxygenase-2. In addition, G-T-R reduced the cytokines secretion such as tumor necrosis factor (TNF-α), interleukin (IL) -1β and IL-6, in BV2 microglia treated with LPS. In addition, G-T-R dose-dependently decreased the activation of nuclear factor-kappa B. These findings confirmed the anti-neuroinflammatory activity of G-T-R, which may exert protective effects against neuroinflammatory-related diseases.

Keywords

Glucose-taurine reduced (G-T-R) BV2 microglia Anti-neuroinflammatory effects 

Abbreviations

NF-κB

nuclear factor-kappa B

BCA

bicinchoninic acid

CNS

central nervous system

COX-2

cyclooxygenase-2

DTT

dithiothreitol

ECL

enhanced chemiluminescence

EGTA

ethylene glycol tetra-acetic acid

FBS

fetal bovine serum

G-T-R

glucose-taurine reduced

HRP

horseradish peroxidase

IL

interleukin

iNOS

inducible nitric oxide synthase

LPS

lipopolysaccharide

MTT

3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide

NO

nitric oxide

PBS

phosphate buffered saline

PGE2

prostaglandin E2

ROS

reactive oxygen species

TNF-α

tumor necrosis factor–alpha

α-MEM

alpha-minimum essential medium

Notes

Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2015R1C1A1A02036465 & NRF-2018R1C1B6001913).

References

  1. Baldwin AS (1996) The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol 14:649–683CrossRefGoogle Scholar
  2. Beynon SB, Walker FR (2012) Microglial activation in the injured and healthy brain: what are we really talking about? Practical and theoretical issues associated with the measurement of changes in microglial morphology. Neuroscience 225:162–171CrossRefGoogle Scholar
  3. Coyle JT, Puttfarcken P (1993) Oxidative stress, glutamate and neurodegenerative disorders. Science 262:689–695CrossRefGoogle Scholar
  4. Dinarello CA (1999) Cytokines as endogenous pyrogens. J Infect Dis 179:294–304CrossRefGoogle Scholar
  5. Gandhi VM, Cherian KM, Mulky MJ (1992) Hypolipidemic action of taurine in rats Indian. J Exp Biol 30(5):413–417Google Scholar
  6. Hoffmann EK, Lambert IH (1983) Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells. Physiol 338:613–625CrossRefGoogle Scholar
  7. Kim JH, Park YM, Shin JS, Park SJ, Choi JH, Jung HJ, Park HJ, Lee KT (2009) Fraxinellone inhibits lipopolysaccharide-induced inducible nitric oxide synthase and cyclooxygenase-2 expression by negatively regulating nuclear factor-kappa B in RAW 264.7 macrophages cells. Biol Pharm Bull 32:1062–1068CrossRefGoogle Scholar
  8. Kim KJ, Yoon KY, Yoon HS, Oh SR, Lee BY (2015) Brazilein suppresses inflammation through inactivation of IRAK4-NF- κ B pathway in LPS-induced raw264.7 macrophage cells. Int J Mol Sci 16:27589–27598CrossRefGoogle Scholar
  9. Lee DS, Jeong GS (2016) Butein provides neuroprotective and antineuroinflammatory effects through Nrf2/ARE-dependent haem oxygenase 1 expression by activating the PI3K/Akt pathway. Br J Pharmacol 173(19):2894–2909CrossRefGoogle Scholar
  10. Li J, Baud O, Vartanian T, Volpe JJ, Rosenberg PA (2005) Peroxynitrite generated by inducible nitric oxide synthase and NADPH oxidase mediates microglial toxicity to oligodendrocytes. Proc Natl Acad Sci USA 102:9936–9941CrossRefGoogle Scholar
  11. Liao C-H, Sang S, Liang Y-C, Ho C-T, Lin J-K (2004) Suppression of inducible nitric oxide synthase and cyclooxygenase-2 in downregulating nuclear factor-kappa B pathway by Garcinol. Mol Carcinog 41(3):140–149CrossRefGoogle Scholar
  12. Lim EY, Kim H (1995) Effect of taurine supplement on the lipid peroxide formation and the activity of glutathione - dependent enzyme in the liver and islet of diabetic model mice. J Koreans Soc Food Nutr 24(2):195–201Google Scholar
  13. Lynch MA (2009) The multifaceted profile of activated microglia. Mol Neurobiol 40:139–156CrossRefGoogle Scholar
  14. Malyshev IY, Shnyra A (2003) Controlled modulation of inflammatory, stress and apoptotic responses in macrophages. Curr Drug Targets Immune Endocr Metab Disord 3:1–22CrossRefGoogle Scholar
  15. McGeer PL, Kawamata T, Walker DG, Akiyama H, Tooyama I, McGeer EG (1993) Microglia in degenerative neurological disease. Glia 7:84–92CrossRefGoogle Scholar
  16. Medzhitov R (2010) Inflammation 2010: new adventures of an old flame. Cell 140:771–776CrossRefGoogle Scholar
  17. Nakamura Y, Si QS, Kataoka K (1999) Lipopolysaccharide-induced microglial activation in culture: temporal profiles of morphological change and release of cytokines and nitric oxide. Neurosci Res 35:95–100CrossRefGoogle Scholar
  18. Park T, Lee K (1997) Effects of dietary taurine supplementation on plasma and liver lipid levels in rats fed a cholesterol-free diet. J Koreans Soc Food Nutr 30(10):1132–1139Google Scholar
  19. Park T, Lee K (1998) Dietary taurine supplementation reduces plasma and liver cholesterol and triglyceride levels in rats fed a high-cholesterol or a cholesterol-free diet. Adv Exp Med Biol 442(Taurine 3):319–325CrossRefGoogle Scholar
  20. Pasantes-Morales H, Cruz C (1985) Taurine and hypotaurine inhibit light-induced lipid peroxidation and protect rod outer segment structure. Brain Res 330(1):154–157CrossRefGoogle Scholar
  21. Romero LI, Tatro JB, Field JA, Reichlin S (1996) Roles of IL-1 and TNF-alpha in endotoxin-induced activation of nitric oxide synthase in cultured rat brain cells. Am J Physiol Regul Integr Comp Physiol 270(2):R326–R332CrossRefGoogle Scholar
  22. Roshak AK, Jackson JR, McGough K, Chanbot-Fletcher M, Mochan E, Marshall LA (1996) Manipulation of distinct NF-κB proteins alters interleukin-1β induced human rheumatoid synovial fibroblast prostaglandin E2 formation. J Biol Chem 271:31496–31501CrossRefGoogle Scholar
  23. Roy A, Park HJ, Abdul QA, Jung HA, Choi JS (2018) Pulegone Exhibits Anti-inflammatory activities through the regulation of NF-κB and Nrf-2 Signaling pathways in LPS-stimulated RAW 264.7 cells. Nat Prod Sci 24(1):28–35CrossRefGoogle Scholar
  24. Satoh T, Lipton SA (2007) Redox regulation of neuronal survival mediated by electrophilic compounds. Trends Neurosci 30:37–45CrossRefGoogle Scholar
  25. Satoh T, Okamoto S, Cui J, Watanabe Y, Furuta K, Suzuki M, Tohyama K, Lipton SA (2006) Activationof the Keap1/Nrf2 pathway for neuroprotection by electrophilic [correction of electrophillic] phase II inducers. Proc Natl Acad Sci USA 103:768–773CrossRefGoogle Scholar
  26. Sohn ES, Sohn EH (2011) A study on the R&D trend and patent analysis of treatments for degenerative brain diseases. J Korea Acad Industr Coop Soc 12:4411–4417CrossRefGoogle Scholar
  27. Stanzione P, Tropepi D (2011) Drugs and clinical trials in neurodegenerative diseases. Ann Ist Super Sanita 47(1):49–54PubMedGoogle Scholar
  28. Xie QW, Kashiwabara Y, Nathan C (1994) Role of transcription factor NF-κB/Rel in induction of nitric oxide synthase. J Biol Chem 269:4705–4708PubMedGoogle Scholar
  29. Yang P, Shen B, Gao Q, Zhu J, Dong J, Zhang L, Zhang Y (2016) Caspase-1 inhibition attenuates activation of BV2 microglia induced by LPS-treated RAW264.7 macrophages. J Biomed Res 30(3):225–233Google Scholar
  30. Yoon JS, Yang H, Kim SH, Sung SH, Kim YC (2010) Limonoids from Dictamnus dasycarpus protect against glutamate-induced toxicity in primary cultured rat cortical cells. J Mol Neurosci 42:9–16CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Hwan Lee
    • 1
  • Dong-Sung Lee
    • 1
  • Kyung Ja Chang
    • 2
  • Sung Hoon Kim
    • 3
  • Sun Hee Cheong
    • 4
  1. 1.College of PharmacyChosun UniversityDong-gu, GwangjuRepublic of Korea
  2. 2.Department of Food and NutritionInha UniversityIncheonRepublic of Korea
  3. 3.Department of ChemistryKonkuk UniversitySeoulRepublic of Korea
  4. 4.Department of Marine Bio-Food SciencesChonnam National UniversityYeosuRepublic of Korea

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