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Taurine 11 pp 847-856 | Cite as

Effect of Taurine on Alterations in Deiodinase 3 Expression Induced by BDE 209 in Human Neuroblasoma-Derived SK-N-AS Cells

  • Xiaona Shang
  • Xiaoying Niu
  • Ruonan Wang
  • Zhenwei Li
  • Xiaoji Hao
  • Xiaohui Liu
  • Jing Shao
  • Lu Han
  • Yachen Li
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

PBDEs (stands for polybrominated diphenyl ethers) are extensively utilized flame retardants, and BDE 209 is one of the most widely used congeners. Studies have suggested the general toxic effects of PBDEs on the endocrine system and neural development. Our previous studies found that BDE 209 changed Type 3 iodothyronine deiodinase (Dio 3) expression in human SK-N-AS neuroblastoma cells. The current study was designed to examine the potential protection of taurine on alterations of Dio 3 expression induced by BDE 209 in SK-N-AS cells. Briefly, SK-N-AS cells were pretreated with taurine prior to the BDE 209 treatment, and the cell viability was evaluated by the MTT (methyl-thiazolyl-tetrazolium) assay. The disturbance or restoration in the levels of Dio 3 proteins and mRNA were observed separately by the western blot and qRT-PCR. Our data showed that taurine moderately attenuated BDE 209-mediated the loss of cell viability. Also, taurine moderately prevented the reduction in the Dio 3 protein expression and mRNA expression induced by BDE 209 in the SK-N-AS cells. Our findings indicated that taurine potentially provide the protection on PBDEs-induced toxicity on endocrine and neuro-development.

Keywords

SK-N-AS cells Polybrominated diphenyl ethers (PBDEs) Taurine Neurotoxicity Deiodinase 3 (Dio 3) 

Abbreviations

BDE 209

Decabromodiphenyl ether

Dio

Deiodinase

MTT

3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide

SK-N-AS

Human SK-N-AS neuroblastoma cells

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81773389/H2601); Dalian Tech Stars Programme (2016RQ044 to XL) and China’s Post-doctoral Science Fund (2016 M591438 to XL).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Xiaona Shang
    • 1
    • 2
  • Xiaoying Niu
    • 1
  • Ruonan Wang
    • 1
  • Zhenwei Li
    • 3
  • Xiaoji Hao
    • 1
  • Xiaohui Liu
    • 1
  • Jing Shao
    • 1
  • Lu Han
    • 4
  • Yachen Li
    • 1
  1. 1.Dalian Key Laboratory of Hematology; Department of Occupational and Environmental Health, School of Public HealthDalian Medical UniversityDalianChina
  2. 2.Pharmaron Beijing Co., Ltd.BeijingChina
  3. 3.Department of Occupational and Environmental Health, School of Public HealthZhejiang Chinese Medical UniversityHangzhouChina
  4. 4.Department of ObstetricsMaternal and Child Care Service Center of DalianDalianChina

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