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Taurine 11 pp 787-799 | Cite as

Anti-apoptotic Effect of Taurine on Schwann Cells Exposed to High Glucose In Vitro

  • Kaixin Li
  • Inam-u-llah
  • Xiaoxia Shi
  • Mengren Zhang
  • Pingan Wu
  • Shuangyue Li
  • Raheel Suleman
  • Azhar Nisar
  • Fengyuan Piao
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

It was reported that apoptosis of Schwann cells could increase in the diabetic rats. The studies showed that taurine inhibited apoptosis in a variety of cells. However, there were few reports on studying the protection of taurine against apoptosis of Schwann cells induced by high glucose (HG) and the underlying mechanism. In our study, the cells were divided into five groups: Control: the normal medium; HG group: 50 mM high glucose; T1: 50 mM high glucose+Taurine (10 mM) group; T2: 50 mM high glucose+Taurine (20 mM) group; T3: 50 mM high glucose+Taurine (40 mM) group. We used MTT and Tunel assays to measure the cell viability and apoptosis, respectively. Then, we also used western blotting to detect the protein levels of apoptosis-related protein. The results demonstrate that taurine promoted cell viability and decreased apoptosis in RSC96 cells exposed to HG. Furthermore, taurine markedly improved imbalance of Bax and Bcl-2, inhibited the translocation of Cytochrome C (Cyt C) from mitochondria to cytosol and reduced caspase-3 activity in HG-induced RSC96 cells. Our results indicate that taurine protect against apoptosis of Schwann cells induced by HG via inhibiting mitochondria-dependent caspase-3 pathway.

Keywords

High glucose Apoptosis Schwann cells Schwann cells Anti-apoptotic effect 

Abbreviations

DM

Diabetes mellitus

HG

High glucose

MTT

4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide

TUNEL

In situ TdT-mediated dUTP nick end labeling

Notes

Conflict of Interest

It is declared that there is no conflict of interest among the authors.

Research Subjects

It is declared that there is no animal was harmed or hurt except according to ethics.

Informed Consent

It is declared that this manuscript is submitting after consent from all authors are aware of this submission.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Kaixin Li
    • 1
  • Inam-u-llah
    • 1
  • Xiaoxia Shi
    • 1
  • Mengren Zhang
    • 1
  • Pingan Wu
    • 1
  • Shuangyue Li
    • 1
  • Raheel Suleman
    • 2
  • Azhar Nisar
    • 3
  • Fengyuan Piao
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Institute of Food Science and Technology, Graduate School of Chinese Academy of Agriculture ScienceBeijingChina
  3. 3.Department of Biochemistry and Molecular BiologyDalian Medical UniversityDalianChina

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