Cytoprotective Effects of an Aqueous Extracts from Atrina Pectinate Meat in H2O2-Induced Oxidative Stress in a Human Hepatocyte
In the present study, we investigated the antioxidant activity of an aqueous extract from Atrina pectinate meat (APW) against H2O2-induced oxidative stress in a human hepatocyte. The extraction yield of APW was 30.01 ± 0.83% and which contained the highest taurine content among free amino acid contents. APW led to the high antioxidant activity showing 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, good reducing power and oxygen radical absorbance capacity (ORAC) value. Also, the results showed that APW improved the cell viability decreased by H2O2 stimulation as well as the reduction of intracellular reactive oxygen species (ROS) generation in hepatocytes. Additionally, APW up-regulated the production of antioxidant mechanisms related enzymes such as catalase and superoxide dismutase (SOD), compared to the only H2O2-treated hepatocytes. Moreover, APW increased the expressions of nuclear Nrf2 and cytosolic HO-1 in H2O2-treated hepatocytes. Interestingly, the treatment of ZnPP, a HO-1 inhibitor abolished the cell viability and intracellular ROS generation induced by APW treatment. In conclusion, this study suggests that APW protects H2O2 induced oxidative stress via up-regulating of Nrf2/HO-1 signal pathway in hepatocytes.
KeywordsAtrina pectinate Anti-oxidant Hepatocytes Nrf2/HO-1 signal pathway
This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(2018R1A6A1A03024314).
- Jian Z, Li K, Liu L, Zhang Y, Zhou Z, Li C, Gao T (2011) Heme oxygenase-1 protects human melanocytes from H2O2-induced oxidative stress via the Nrf2-ARE pathway. J Invest Dermatol 131:1420–1142Google Scholar
- Lee W, Kim EA, Um JH, Kang N, Han EJ, Oh JY, Park SY, Jeon YJ, Ahn G (2017) Radio-protective effects of Octopus ocellatus meat consisted of a plentiful Taurine against damages caused by gamma ray irradiation. In: Taurine 10. Springer, pp 955–971Google Scholar
- Mohibbullah M, Won NE, Jeon JH, An JH, Park Y, Kim H, Bashir KMI, Park SM, Kim YS, Yoon SJ, Sohn JH, Kim A, Choi JS (2018) Effect of superheated steam roasting with hot smoking treatment on improving physicochemical properties of the adductor muscle of pen shell (Atrina pectinate). Food Sci Nutr 6(5):1317–1327CrossRefGoogle Scholar
- Um JH, Kim EA, Lee W, Kang N, Han EJ, Oh JY, Park SY, Jeon YJ, Lee SH, Ahn G (2017) Protective effects of an enzymatic hydrolysate from Octopus ocellatus meat against hydrogen peroxide-induced oxidative stress in chang liver cells and Zebrafish embryo. In: Taurine 10. Springer, pp 603–620Google Scholar