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Taurine 11 pp 163-172 | Cite as

Effect of Taurine on Cell Function via TXNIP Induction in Caco-2 Cells

  • Hideo SatsuEmail author
  • Yusuke Gondo
  • Hana Shimanaka
  • Kenji Watari
  • Midori Fukumura
  • Makoto Shimizu
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Taurine (2-aminoethanesulfonic acid), a sulfur-containing β-amino acid, is a free amino acid present in high concentrations in mammalian tissues. Taurine has pivotal roles in anti-oxidation, membrane stabilization, osmoregulation, anti-inflammation, and other process. In a DNA microarray analysis, we previously found that taurine markedly increases the mRNA expression of thioredoxin interacting protein (TXNIP) in Caco-2 cells. In this study, we investigated the effect of these taurine-induced changes in TXNIP on the function of Caco-2 cells. We found that taurine decreases glucose uptake in a dose-dependent manner. The taurine-induced decrease in glucose uptake was completely abolished by transfection with siRNA against TXNIP, suggesting that TXNIP is involved in the taurine-induced down-regulation of glucose uptake. We also revealed that taurine induces AMPK activation and further increases the intracellular ATP content in Caco-2 cells. These results suggest that taurine could regulate the function of Caco-2 cells via TXNIP induction, leading to extend our understanding of the functions of taurine.

Keywords

TXNIP Caco-2 GLUT AMPK ATP 

Abbreviations

TXNIP

thioredoxin interacting protein

AMP

adenosine monophosphate

AMPK

adenosine monophosphate kinase

ATP

adenosine triphosphate

GLUT1

glucose transporter 1

GLUT3

glucose transporter 3

ChoRE

carbohydrate response element

ChREBP

carbohydrate response element binding protein

mTOR

mammalian target of rapamycin

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Hideo Satsu
    • 1
    Email author
  • Yusuke Gondo
    • 2
  • Hana Shimanaka
    • 2
  • Kenji Watari
    • 1
  • Midori Fukumura
    • 1
  • Makoto Shimizu
    • 3
  1. 1.Department of BiotechnologyMaebashi Institute of TechnologyMaebashiJapan
  2. 2.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  3. 3.Department of Nutritional ScienceTokyo University of AgricultureTokyoJapan

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