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Taurine 11 pp 113-118 | Cite as

The Production of a Rat Model That Inhibits Phosphoenolpyruvate Carboxykinase (PEPCK), a Rate-Limiting Enzyme of Hepatic Gluconeogenesis

  • Hajime OhmoriEmail author
  • Masataka Matsumura
  • Shoichi Komine
  • Haruki Kobayashi
  • Yusei Kobayashi
  • Jun Shiromoto
  • Sumpei Miyakawa
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

We previously showed that taurine administration contributed to the extension of time to exhaustion through exercise-induced hypoglycemia restraint, and we suggested that the activation of hepatic gluconeogenesis was initiated before the exercise with the taurine administration. We hypothesize that the extension effect of exercise duration with the taurine administration is restrained in the rats which inhibited hepatic gluconeogenesis. In this study, we aimed to produce a rat model that inhibited hepatic gluconeogenesis as a first step in testing our hypothesis.

F344 male rats of 8 weeks after birth were purchased. The blood samples were collected via jugular vein catheter to perform the pyruvate tolerance test (PTT) with the intraperitoneal administration, and to determine the optimal time point of blood glucose measurement. 3-mercaptopicolinic acids (3MPA) was used as an inhibitor of PEPCK. The rats were divided into three groups, Non-dosage control (CON) group, 30 mg/kg・BW 3MPA (3MPA 30) group, and 300 mg/kg・BW 3MPA (3MPA 300) group.

The blood glucose level showed a significant peak 15 min after pyruvate administration. The change of the blood glucose level after the PTT in 3MPA 300 group was significantly smaller than that of the CON group at this time point. These results show we could prepare the rat model that inhibited hepatic gluconeogenesis.

Keywords

Hepatic glucose production Prolonged exercise Running performance 

Abbreviations

PTT

pyruvate tolerance test

3MPA

3-mercaptopicolinic acids

G6Pase

glucose 6-phosphatase

PEPCK

phosphoenolpyruvate carboxykinase

References

  1. Coggan AR, Coyle EF (1987) Reversal of fatigue during prolonged exercise by carbohydrate infusion or ingestion. J Appl Physiol 63(6):2388–2395CrossRefGoogle Scholar
  2. DiTullio NW, Berkoff CE, Blank B, Kostos V, Stack EJ, Saunders HL (1974) 3-mercaptopicolinic acid, an inhibitor of gluconeogenesis. Biochem J 138(3):387–394CrossRefGoogle Scholar
  3. Ito T, Kimura Y, Uozumi Y, Takai M, Muraoka S, Matsuda T, Ueki K, Yoshiyama M, Ikawa M, Okabe M, Schaffer SW, Fujio Y, Azuma J (2008) Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy. J Mol Cell Cardiol 44(5):927–937CrossRefGoogle Scholar
  4. Miyazaki T, Matsuzaki Y, Ikegami T, Miyakawa S, Doy M, Tanaka N, Bouscarel B (2004) Optimal and effective oral dose of taurine to prolong exercise performance in rat. Amino Acids 27(3–4):291–298CrossRefGoogle Scholar
  5. Turcotte LP, Brooks GA (1990) Effects of training on glucose metabolism of gluconeogenesis-inhibited short-term-fasted rats. J Apple Physiol 68(3):944–954CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Hajime Ohmori
    • 1
    Email author
  • Masataka Matsumura
    • 1
  • Shoichi Komine
    • 1
  • Haruki Kobayashi
    • 1
  • Yusei Kobayashi
    • 1
  • Jun Shiromoto
    • 1
  • Sumpei Miyakawa
    • 1
  1. 1.Field of Exercise Biochemistry, Faculty of Health and Sport SciencesUniversity of TsukubaTsukubaJapan

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