Molecular Breeding

  • P. M. Priyadarshan


Application of molecular biology in plant breeding is molecular breeding. The process of developing new crop varieties through conventional means can take almost 25 years, but the application of biotechnology has considerably shortened the time to 7–10 years for deriving new crop varieties for commercial exploitation. One of the tools for easier and faster selection of plant traits is marker-assisted selection (MAS). The areas of molecular breeding include QTL mapping or gene discovery, marker-assisted selection and genomic selection, genetic engineering and genetic transformation.


What Are Molecular Markers? Genetic Markers Classical Markers DNA Markers Summary of Major Classes of Genetic Markers Prerequisites for Molecular Breeding Activities of Marker-Assisted Breeding What is Mapping? MAS for Qualitative Traits MAS for Quantitative Traits QTL Detection (Statistical) Next-Generation Molecular Breeding Next-Generation Sequencing (NGS) Genotyping-by-Sequencing (GBS) RFLP, and AFLP as Tools to Map Genomes RAPD Technique Genetic Maps Physical Maps 

Further Reading

  1. Arif IA (2010) A brief review of molecular techniques to assess plant diversity. Int J Mol Sci 11:2079–2096. Scholar
  2. Birchler JA, Han F (2018) Barbara McClintock’s Unsolved Chromosomal Mysteries: Parallels to Common Rearrangements and Karyotype Evolution. Plant Cell 30:771–779CrossRefGoogle Scholar
  3. Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil Trans R Soc B 363:557–572. Scholar
  4. Dunwell JM (2011) Crop biotechnology: prospects and opportunities. J Agric Sci 149(S1):17–29. ISSN 1469-5146. Scholar
  5. Nybom et al (2014) DNA fingerprinting in botany: past, present, future. Investig Genet 5:1–35CrossRefGoogle Scholar
  6. Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 18:7213–7218CrossRefGoogle Scholar
  7. Xu Y (2010) Molecular plant breeding. CABIGoogle Scholar
  8. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • P. M. Priyadarshan
    • 1
  1. 1.Erstwhile Deputy DirectorRubber Research Institute of IndiaKottayamIndia

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