Compatibility Potential of Brassica Species and Mustard Seed Meal with Pseudomonas fluorescens for Biological Control of Soilborne Plant Diseases

  • Bindu Madhavi Gopireddy
  • Uma Devi Gali
  • Vijay Krishna Kumar Kotamraju
  • Ramesh Babu Tatinaeni
  • China Muniswamy Naidu


The biofumigant potential of different Brassica sp. and onion for compatibility with PGPR strain of Pseudomonas fluorescens under in vitro conditions was studied. The local varieties of biofumigant crops, viz., cabbage, cauliflower, mustard, and onion, were grown in pots under greenhouse conditions. Treatments included were macerated tissue containing shoots, roots, and leaves alone and in combination with one another. Actively growing P. fluorescens cultures were streaked onto the inverted bottom of the Petri plate filled with nutrient agar and kept above the Petri plate containing macerated plant tissue in different treatments at room temperature for 48–72 h. Results showed that there was no reduction of CFU per plate compared with untreated control. No statistically significant effect was recorded for any of the amended plant material tested against P. fluorescens growth. In another study, the growth of P. fluorescens was observed for 72 h after continuous exposure to volatiles produced by hydrated mustard seed meal (different quantities) under in vitro conditions. Fungistatic effect was not observed for P. fluorescens growth against different concentrations of seed meal, and this attributed tolerance of P. fluorescent toxic volatiles produced by seed meal. This work could be important in the future for the integrated use of biofumigants/mustard seed meal along with P. fluorescens for the management of plant diseases.


Compatibility Biofumigation Brassica species Mustard seed meal Pseudomonas fluorescens 



The authors are grateful to Vice Chancellor, Acharya N G Ranga Agricultural University, Lam, Guntur, Andhra Pradesh, India, for their support.


  1. Angus JF, Gardner PA, Kirkegaard JA, Desmarchelier JM (1994) Biofumigation: isothiocyanates released from Brassica roots inhibit the growth of the take-all fungus. Plant Soil 162:107–112CrossRefGoogle Scholar
  2. Bellostas N, Sorensen JC, Sorensen H (2007) Profiling glucosinolates in vegetative and reproductive tissues of four Brassica species of the U-triangle for their biofumigation potential. J Sci Food Agric 87:1586–1594CrossRefGoogle Scholar
  3. Blok WJ, Lamers JG, Termorshuizen AJ, Bollen GJ (2000) Control of soilborne plant pathogens by incorporating fresh organic amendments followed by tarping. Phytopathology 90:253–259CrossRefGoogle Scholar
  4. Borek V, Morra MJ (2005) Ionic thiocyanate (SCN-) production from 4-hydroxybenzyl glucosinolate contained in Sinapis alba seed meal. J Agric Food Chem 53:8650–8654CrossRefGoogle Scholar
  5. Brown PD, Morra MJ (1997) Hydrolysis products of glucosinolates in Brassica napus tissues as inhibitors of seed germination. Plant Soil 181:307–316CrossRefGoogle Scholar
  6. Brown PD, Tokuhisa JG, Reichelt M, Gershenzon J (1991) Variation of glucosinolate accumulation among different organs and development stages of Arabidopsis thaliana. Phytochemistry 62:471–481CrossRefGoogle Scholar
  7. Challenger F (1959) Aspects of the organic chemistry of sulphur. Butterworths, LondonGoogle Scholar
  8. Charron CS, Sams CE (1999) Inhibition of Pythium ultimum and R. solani by shredded leaves of Brassica species. J Am Soc Hortic Sci 124(5):462–467CrossRefGoogle Scholar
  9. Cohen MF, Mazzola M (2006) Resident bacteria, nitric oxide emission, and particle size modulate the effect of Brassica napus seed meal on disease incited by R. solani and Pythium sp. Plant Soil 286(1–2):75–86CrossRefGoogle Scholar
  10. Cohen MF, Yamasaki H, Mazzola M (2005) Brassica napus seed meal soil amendment modifies microbial community structure, nitric oxide production and incidence of Rhizoctonia root rot. Soil Biol Biochem 37:1215–1227CrossRefGoogle Scholar
  11. Dhingra OD, Costa MLN, JR Silva JG (2004) Potential of allyl isothiocyanate to control Rhizoctonia solani seedling damping off and seedling blight in transplant production. J Phytopathol 152:352–357CrossRefGoogle Scholar
  12. Duniway J (2002) Chemical alternatives to methyl bromide for soil treatment particularly in strawberry production. In: Proceedings of international conference on alternatives to methyl bromide, Seville, Spain, p 432Google Scholar
  13. Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5–51CrossRefGoogle Scholar
  14. Fayzalla EA, El-Barougy E, El-Rayes MM (2009) Control of soil-borne pathogenic fungi of soybean by biofumigation with mustard seed meal. J Appl Sci 9:272–279CrossRefGoogle Scholar
  15. Friberg H, Edel-Hermann V, Faivre C, Gautheron N, Fayolle L, Faloya V, Montfort F, Steinberg C (2009) Cause and duration of mustard incorporation effects on soil-borne plant pathogenic fungi. Soil Bioland Biochem 41:2075–2084CrossRefGoogle Scholar
  16. Galletti S, Sala E, Leoni Burzi O, Cerato PLC (2008) Trichoderma sp. tolerance to Brassica carinata seed meal for a combined use in biofumigation. Biol Control 45(3:319–327CrossRefGoogle Scholar
  17. Gardiner JB, Morra MJ, Eberlein CV, Brown PD, Borek V (1999) Allelochemicals released in soil following incorporation of rapeseed (Brassica napus) green manures. J Agric Food Chem 47:3837–3842CrossRefGoogle Scholar
  18. Gimsing AL, Kirkegaard JA (2006) Glucosinolate and isothiocyanate concentration in soil following incorporation of Brassica biofumigants. Soil Biol Biochem 38:2255–2264CrossRefGoogle Scholar
  19. Gimsing AL, Kirkegaard JA (2008) Glucosinolates and biofumigation: the fate of glucosinolates and their hydrolysis products in soil. Phytochem Rev 8(1):299–310CrossRefGoogle Scholar
  20. Gimsing AL, Sorensen JC, Tovgaard L, Jorgensen AMF, Hansen HCB (2006) Degradation kinetics of glucosinolates in soil. Environ Toxicol Chem 25(8):2038–2044CrossRefGoogle Scholar
  21. Hoagland L, Carpenter-Boggs L, Reganold JP, Mazzola M (2008) Role of native soil biology in brassicaceous seed meal-induced weed suppression. Soil Biol Biochem 40(7):1689–1697CrossRefGoogle Scholar
  22. Kirkegaard JA, Sarwar M (1998) Biofumigation potential of Brassicas I. Variation in glucosinolate profiles of diverse field-grown Brassicas. Plant Soil 201:71–89CrossRefGoogle Scholar
  23. Kirkegaard AJ, Gardener AP, Desmarchelier MJ, Angus FJ (1993) Biofumigation -using Brassica species to control pests and diseases in horticulture and agriculture. In: 9th Austrailian Research Assembly on Brassicas, pp 77–82Google Scholar
  24. Kirkegaard JA, Wong PTW, Desmarchelier JM (1996) In vitro suppression of fungal root pathogens of cereals by Brassica tissues. Plant Pathol 45:593–603CrossRefGoogle Scholar
  25. Kirkegaard JA, Sarwar M, Matthiessen JN (1998) Assessing the biofumigation potential of crucifers. Acta Hortic 459:105–111CrossRefGoogle Scholar
  26. Kirkegaard JA, Sarwar M, Wong PTW, Mead A, Howe G, Newell M (2000) Field studies on the biofumigation of take-all by Brassica break crops. Aus J Agric Res 51:445–456CrossRefGoogle Scholar
  27. Kreutzer WA (1963) Selective toxicity of chemicals to soil microorganisms. Annu Rev Phytopathol 1:101–126CrossRefGoogle Scholar
  28. Larkin RP, Griffin TS (2007) Control of soilborne diseases of potato using Brassica green manures. Crop Prot 26:1067–1077CrossRefGoogle Scholar
  29. Lazzeri L, Curto G, Leoni O, Dallavalle E (2004) Effects of glucosinolates and their enzymatic hydrolysis products via myrosinase on the root-knot nematode Meloidogyne incognita (Kofoid et white) hit. J Agric Food Chem 52:6703–6707CrossRefGoogle Scholar
  30. Madhavi GB, Umadevi G (2018) Effect of combined application of biofumigant, Trichoderma harzianum and Pseudomonas fluorescens on Rhizoctonia solani f. sp. sasakii. Indian Phytopath 71(2):257–263. CrossRefGoogle Scholar
  31. Madhavi GB, Umadevi G, Kumar KVK, Babu TR, Naidu TCM (2015) Evaluation of different Brassica species and onion for their biofumigation effect against Rhizoctonia solani f. sp. sasakii in vitro. J Res ANGRAU 43(3&4):22–28Google Scholar
  32. Madhavi GB, Umadevi G, Kumar KVK, Babu TR, Naidu TCM (2016) Effect of volatiles produced by hydrolysis of mustard seed powder against Rhizoctonia solani f. sp. sasakii, Trichoderma harzianum, and Pseudomonas fluorescens. Prog Res 11(Special- VII):4821–4823Google Scholar
  33. Matthiessen JN, Kirkegaard JA (2002) Potato grower’s positive experiences with biofumigant green manure. Horti Biofumi Update 2Google Scholar
  34. Matthiessen JN, Kirkegaard JA (2006) Biofumigation and enhanced biodegradation: opportunity and challenge in soilborne pest and disease management. Crit Rev Plant Sci 25:235–265CrossRefGoogle Scholar
  35. Mattner SW, Porter IJ, Gounder RK, Shanks AL, Wren DJ, Allen D (2008) Factors that impact on the ability of biofumigants to suppress fungal pathogens and weeds of strawberry. Crop Prot 27:1165–1173CrossRefGoogle Scholar
  36. Mayton SH, Olivier C, Vaughn FS, Loria R (1996) Correlation of fungicidal activity of Brassica species with allyl isothiocyanate production in macerated leaf tissue. Am Phytopathol Soci 86:267–271CrossRefGoogle Scholar
  37. Mazzola M, Zhao X (2010) Brassica juncea seed meal particle size influence the chemistry but not soil biology based suppression of individual agents inciting apple to replant disease. Plant Soil 337:313–324CrossRefGoogle Scholar
  38. Mazzola M, Granatstein DM, Elfving DC, Mullinix K (2001) Suppression of specific apple root pathogens by Brassica napus seed meal amendment regardless of glucosinolate content. Phytopathology 91(7):673–679CrossRefGoogle Scholar
  39. Motisi N, Montfort F, Dore T, Romillac N, Lucas P (2009) Duration of control of two soilborne pathogens following incorporation of above and below ground residues of Brassica juncea into soil. Plant Pathol 58:470–478CrossRefGoogle Scholar
  40. Motisi N, Doré T, Lucas P, Montfort F (2010) Dealing with the variability in biofumigation efficacy through an epidemiological framework. Soil Biol Biochem 42:2044–2057CrossRefGoogle Scholar
  41. Omirou M, Karpouzas DG, Papadopoulou KK, Ehaliotis C (2013) decomposition of pure and plant derived glucosinolate in soil. Eur J Soil Biol 56:49–55CrossRefGoogle Scholar
  42. Perez C, Dill-Macky R, Kinkel LL (2007) Management of soil microbial communities to enhance populations of Fusarium graminearum- antagonists in the soil. Plant Soil 302(1–2):53–69Google Scholar
  43. Porter IJ, Mattner S (2002) Non-chemical alternatives to methyl bromide for soil treatment in strawberry production. In: Proceedings of international conference on Alternatives to Methyl Bromide, March 5–8, Spain, 432, pp 39–48Google Scholar
  44. Poulsen JL, Gimsing AL, Halkier BA, Bjarnholt N, Hansen HCB (2008) Mineralization of benzyl glucosinolate and its hydrolysis product the biofumigant benzyl isothiocyanate in the soil. Soil Biol Biochem 40(1):135–141CrossRefGoogle Scholar
  45. Raaijmakers JM, Paulitz TC, Steinberg C, Alabouvette C, Moënne-Loccoz Y (2009) The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321:341–361CrossRefGoogle Scholar
  46. Rahmanpour S, Backhouse D, Nonhebel HM (2009) Induced tolerance of Sclerotinia sclerotiorumto isothiocyanates and toxic volatiles from Brassica species. Plant Pathol 58:479–486CrossRefGoogle Scholar
  47. Rosa ASE (1997) Daily variation in glucosinolate concentrations in the leaves and roots of cabbage seedlings in two constant temperature regimes. J Sci Food Agric 73:364–368CrossRefGoogle Scholar
  48. Sarwar M, Kirkegaard JA, Wong PTW, Desmarchelier JM (1998) Biofumigation potential of brassicas III. In vitro toxicity of isothiocyanates to soil-borne fungal pathogens. Plant Soil 201:103–112CrossRefGoogle Scholar
  49. Sellam A, Lacomi-Vasilescu B, Hudhomme P, Simoneau P (2006) In vitro antifungal activity of brassinin, camalexin and two isothiocyanates against the crucifer pathogens Alternaria brassicicola and Alternaria brassicae. Plant Pathol 56:296–301CrossRefGoogle Scholar
  50. Smith BJ, Kirkegaard JA (2002) In vitro inhibition of soil microorganisms by 2-phenyl ethyl isothiocyanate. Plant Pathol 51:585–593CrossRefGoogle Scholar
  51. Smolinska U, Knudsen GR, Morra MJ, Borek V (1997) Inhibition of Aphanomyces euteiches f. sp. by volatiles produced by hydrolysis of Brassica napus seed meal. Plant Dis 81:288–292CrossRefGoogle Scholar
  52. Smolinska U, Morra MJ, Knudsen GR, James RL (2003) Isothiocyanates produced by Brassicaceae species as inhibitors of Fusarium oxysporum. Plant Dis 87:407–412CrossRefGoogle Scholar
  53. Steffek R, Spornberger A, Altenburger J (2006) Detection of microsclerotia of Verticillium dahlia in soil samples and prospects to reduce the inoculum potential of the fungus in the soil. Agric Conspec Sci 71(4):145–148Google Scholar
  54. Vera C, McGergor D, Downey R (1987) Detrimental effects of volunteer Brassica on the production of certain cereal, and oilseed crops. Can J Plant Sci 67(4):983–995CrossRefGoogle Scholar
  55. Walker CJ, Morell S, Foster HH (1937) Toxicity of mustard oils and related Sulphur compounds to certain fungi. Am J Bot 24:241–536Google Scholar
  56. Wathelet J, Lori R, Leoni O, Rollin P, Quinsac A, Palmieri S (2004) Guidelines for glucosinolate analysis in green tissues used for biofumigation. Agroindustria 3:257–266Google Scholar
  57. Williams Woodward JL, Pfleger FL, Fritz VA, Allmaras RR (1997) Green manures of oat, rape and sweet corn for reducing common root rot in pea (Pisum sativum) caused by Aphanomyces euteiches. Plant Soil 188(1):43–48CrossRefGoogle Scholar
  58. Willis RJ (1985) The historical bases of the concept of allelopathy. J Hist Biol 18(1):71–102CrossRefGoogle Scholar
  59. Yulianti Y, Sivasithamparam K, Turner WD (2006) Response of different forms of propagules of Rhizoctonia solani AG2-1 (ZG5) exposed to the volatiles produced in soil amended with green manures. Ann Appl Biol 148:105–111CrossRefGoogle Scholar
  60. Yulianti T, Sivasithamparam K, Turner DW (2007) Saprophytic and pathogenic behavior of R. solani AG2-1 (ZG-5) in a soil amended with Diplotaxis tenuifolia or Brassica nigra manures and incubated at different temperatures and soil water content. Plant Soil 294:277–289CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Bindu Madhavi Gopireddy
    • 1
  • Uma Devi Gali
    • 2
  • Vijay Krishna Kumar Kotamraju
    • 1
  • Ramesh Babu Tatinaeni
    • 1
  • China Muniswamy Naidu
    • 1
  1. 1.Acharya N G Ranga Agricultural UniversityGunturIndia
  2. 2.Department of Plant PathologyCollege of AgricultureRajendranagar, HyderabadIndia

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