Advertisement

Characterization of Sugarcane Mosaic Disease and Its Management with PGPR

  • N. Raja Kumar
  • K. Vijay Krishna Kumar
  • B. Ravindra Reddy
Chapter

Abstract

Sugarcane mosaic caused by sugarcane mosaic virus (SCMV) is a serious problem in India’s sugarcane production. SCMV was first reported in India from Pusa during 1921 in sugarcane variety D-99, and now it has been reported in every sugarcane-growing areas across India due to its perpetuation through vegetative cuttings and regarded it as a potential threat to sugarcane industry. Our present study was focused on the characterization of SCMV and use of PGPR strains to manage the disease. A survey was undertaken in sugarcane-grown areas of Andhra Pradesh, India, and found the sugarcane aphid (Melanaphis sacchari) and corn leaf aphid (Rhopalosiphum maidis) as potential vectors for ScMV. Vector transmission was confirmed using DAC-ELISA. Further, the ScMV was detected in diseased leaves through DAC-ELISA and RT-PCR during our survey. Scanning electron microscopy (SEM) was also used to detect ScMV from diseased leaf samples. The results showed that all the leaf samples collected showed a positive reaction to the presence of ScMV in RT-PCR with a band at around 0.98 kbp. Further, asymptomatic leaves also have shown a positive reaction with RT-PCR for the presence of ScMV. Whereas, SEM studies showed the presence of potyvirus filamentous particles related to ScMV. To manage ScMV, several PGPR strains were isolated from the rhizosphere of sugarcane samples collected during our surveys using standard isolation methods. Studies are being undertaken to identify these strains and further to evaluate their efficacy against ScMV in sugarcane under in vitro and in vivo conditions.

Keywords

Sugarcane mosaic virus (SCMV) Biocontrol PGPR Melanaphis sacchari Rhopalosiphum maidis vectors 

References

  1. Alegria O, Royer M, Bousalem M, Chatenet M, Peterschmitt M, Girard J-C, Rott P (2003) Genetic diversity in the coat protein coding region of eighty-six sugarcane mosaic virus isolates from eight countries, particularly from Cameroon and Congo. Arch Virol 148:357–372CrossRefGoogle Scholar
  2. Bhargava KS (1975) Sugarcane mosaic—retrospect and prospects. Indian Phytopathol 28:1–9Google Scholar
  3. Chetenet M, Mazartin JC, Girard E, Fernandez D, Rao GP, Royer B, Lock Hart B and Rotti P (2005) Detection of sugarcane streak mosaic virus in sugarcane from several Asian countries. Proc ISSCT (25) 2005:656–663Google Scholar
  4. Dastur JF (1923) The mosaic disease of sugarcane in India. Agric J India 18:505–509Google Scholar
  5. Guru Prasad M, Vijay Krishna Kumar K, Bhaskara Reddy BV, Reddi Kumar M, Sai Sruthi V, Sivaprasad Y, Sujitha A, Naga Madhuri KV, Hemalatha TM, Raja Reddy K (2012) Studies on characterization and enhancement of seedling vigor by certain Plant Growth-Promoting Rhizobacteria (PGPR) in sugarcane. Abstract presented at National Conference on “Modern Innovations and Approaches in Biotechnology (NCMIAB-2012), Organized by Dept. of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati-517502 from 29th Feb to 1st Mar 2012Google Scholar
  6. Hema M, Savithri HS, Sreenivasulu P (2003) Comparison of direct binding polymerase chain reaction with the recombinant coat protein antibody-based dot-immunobinding assay and immunocapture-reverse transcription-polymerase chain reaction for the detection of sugarcane streak mosaic disease in India. Curr Sci 85:1774–1777Google Scholar
  7. India Sugar Annual (2016) Global Agricultural Information Network, USDA Foreign Agricultural Service. GAIN Report Number IN6057 (12 Pages) pp 3Google Scholar
  8. Kumar VK, K Yellareddygari SKR, Reddy MS, Kloepper JW, Lawrence KS, Zhou XG, Sudini H, Groth DE, Krishnam Raju S, Miller ME (2012) Efficacy of Bacillus subtilis MBI 600 against sheath blight caused by Rhizoctonia solani and on growth and yield of rice. Rice Sci 19(1):55–63CrossRefGoogle Scholar
  9. Lockhart BEL, Autrey JC, Comstock JC (1992) Partial purification and serology of sugarcane mild mosaic virus, mealy bug-transmitted clusters like a virus. Phytopathology 82:691–695CrossRefGoogle Scholar
  10. Marie-Jeanne V, Loos R, Peyre J, Alliot B, Signoret P (2000) Differentiation of Poaceaepotyviruses by reverse transcription-polymerase chain reaction and restriction analysis. J Phytopathol 148:141–151CrossRefGoogle Scholar
  11. Raja Kumar N, Suresh M, Vijaya Krishna Kumar K, Kishore Varma P, Veerabhadra Rao K (2016a) Sugarcane mosaic disease- a minor disease becoming a major threat to sugarcane cultivation in Andhra Pradesh. Plant Dis Res 31(2):213Google Scholar
  12. Raja Kumar N, Suresh M, Vijayakrishna Kumar K, Kishore Varma P, Veerabhadra Rao K (2016b) Distribution and Characterization of Sugarcane Mosaic Disease in Andhra Pradesh. International Conference and Exhibition on Sugarcane Value Chain-Vision 2025 Sugar, held at VSI, Pune from November 11–16th 2016, pp. 33Google Scholar
  13. Rao GP, Rishi N, Bhargava KS (1995) Status of sugarcane mosaic virus research in India. In: Mishra AS, Prakash O, Agnihotri VP (eds) Disease scenario of plants, vol II. International Books & Periodicals Supply Service, Pitampura, pp 222–234Google Scholar
  14. Rao GP, Singh M, Rishi N, Bhargava KS (2002) Century status of sugarcane virus diseases research in India. In: Singh SB, Rao GP, Easwaramoorthy S (eds) Sugarcane crop management. SCI Tech Publishing LLC, Houstan, pp 223–254Google Scholar
  15. Singh M, Singh A, Upadhyaya PP, Rao GP (2005) Transmission studies on an Indian isolate of sugarcane mosaic potyvirus. Sugar Tech 5(2&3):32–38CrossRefGoogle Scholar
  16. Viswanathan R, Balamuralikrishnan M (2005) Impact of mosaic infection on growth and yield of sugarcane. Sugar Tech 7(1):61–65CrossRefGoogle Scholar
  17. Viswanathan R, Rao GP (2011) Disease scenario and management of major sugarcane diseases in India. Sugar Tech 13(4):336–353CrossRefGoogle Scholar
  18. Viswanathan R, Balamuralikrishnan M, Karuppaiah R (2007) Association of sugarcane mosaic virus and sugarcane streak mosaic virus with sugarcane mosaic in India. Sugar Cane Int 25(2):10–18Google Scholar
  19. Viswanathan R, Balamuralikrishnan M, Karuppaiah R (2008) Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane. Virus Genes 36:553–564CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • N. Raja Kumar
    • 1
  • K. Vijay Krishna Kumar
    • 1
  • B. Ravindra Reddy
    • 1
  1. 1.Acharya N G Ranga Agricultural University, Regional Agricultural Research StationAnakapalleIndia

Personalised recommendations