Production, Optimization, and Characterization of Siderophore by Pseudomonas aeruginosa (C3) Isolated from Rhizospheric Soil

  • Chhaya Verma
  • Apoorva Dixit
  • Rajesh Kumar


In this study production of siderophore was analyzed in fluorescent pseudomonad named as C3 (Pseudomonas aeruginosa). Out of ten isolates, this strain was selected for optimization of siderophore production; it produced 51.45 SU (siderophore unit). Findings of this experiment showed that siderophore production was increased when growth medium was added with potassium nitrate, 0.073 mg/ml FeCl3, 200 ppm cadmium, sucrose (C source), and 0.5% NaCl in shaking condition at pH 6 after 5 days of incubation. Siderophore production was increased with incubation time and cadmium concentration, but after 6 days, siderophore production was reduced. Further, siderophore extraction was done by crystallization process, formed by adding methanol after maintaining pH 3 by mixing of sulfuric acid, ferrous sulfate, and 50% ammonium sulfate. Produced crystal was purified and characterized TLC (thin layer chromatography) and FTIR (Fourier-transform infrared spectroscopy) analysis. In TLC analysis the specific spot from the extracted siderophore was found to correspond with a spot of standard siderophore with the same Rf value. In FTIR analysis similar peak was observed as reported in earlier studies. This study was based on siderophore production, optimization, and characterization of extracted siderophore.


Siderophore Pseudomonas Optimization FTIR 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Chhaya Verma
    • 1
  • Apoorva Dixit
    • 1
  • Rajesh Kumar
    • 1
  1. 1.Rhizosphere Biology Laboratory, Department of Environmental MicrobiologyBabasaheb Bhimrao Ambedkar University (A Central University)LucknowIndia

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