Exploration of Biocontrol and Growth-Promoting Activity of Bacterial Strains Isolated from the Sugarcane Crop

  • Beenu Shastri
  • Anil Kumar
  • Rajesh Kumar


Naturally occurring bacteria were isolated from the internal tissues of stalks as well as from roots of sugarcane crop and from the rhizospheric soil. The highest numbers of bacterial populations were isolated from the rhizospheric zone. Isolated bacterial strains were subjected to antagonistic activity in vitro against Colletotrichum falcatum fungus causing red rot disease in sugarcane crop. Most of the isolated bacteria showed antagonistic activity against C. falcatum in vitro. Isolated antifungal isolates were identified morphologically and biochemically. Further, the potential strains were examined for various plant growth promoting traits and hydrolytic enzymes production. Bacteria isolated from rhizospheric as well as from endophytic zone of sugarcane crop showed the inhibition of red rot pathogen as well showed the in vitro plant growth promotory traits. Thus, isolates help in biocontrol of red rot as well as can be used for increment of sugarcane yield.


Sugarcane Endophytes Rhizospheric bacteria Colletotrichum falcatum 



The authors acknowledge Dr. Ram Ji Lal, Prinicipal Scientist (now retired) and Dr. Dinesh Singh (Principal Scientist), IISR, Lucknow for providing various assistance.


  1. 1.
    Food and Agricultural Organization (FAO) of United Nations: Economic and Social Department: The Statistical Division (2014)Google Scholar
  2. 2.
    Vishwakarma SK, Kumar P, Nigam A et al (2013) Pokkah Boeng: an emerging disease of sugarcane. J Plant Pathol Microbiol 4:170Google Scholar
  3. 3.
    Satyavir S (2003) Red rot of sugarcane – current Scenario. Indian Phytopathol 56:245–254Google Scholar
  4. 4.
    Duttamajumder SK (2008) Red rot of sugarcane. Indian Institute of Sugarcane Research, LucknowGoogle Scholar
  5. 5.
    Hussnain Z, Afghan S (2006) Impact of major cane diseases on sugarcane yield and sugar recovery, Annual report. Shakarganj Sugar Research Institute, JhangGoogle Scholar
  6. 6.
    Viswanathan R, Rajitha R, Sundar RA, Ramamoorthy V (2003) Isolation and Identification of Endophytic Bacterial Strains from Sugarcane Stalks and Their In Vitro Antagonism against the Red Rot Pathogen. Sugartech 5(l & 2):25–29Google Scholar
  7. 7.
    Krieg NR, Holt JG (1984) Bergey’s manual of determinative bacteriology, vol 1. The Williams and Wilkins Co, BaltimoreGoogle Scholar
  8. 8.
    Gordon SA, Weber RP (1951) Colorimetric estimation of indole-acetic acid. Plant Physiol 26:192–195CrossRefGoogle Scholar
  9. 9.
    Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiol 17:362–370Google Scholar
  10. 10.
    Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of Siderophore. Anal Biochem 160:47–56CrossRefGoogle Scholar
  11. 11.
    Dye DW (1962) The inadequacy of the usual determinative tests for identification of Xanthomonas sp. New Zeal J Sci 5:393–416Google Scholar
  12. 12.
    Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation. Soil Biol Biochem 19:451–457CrossRefGoogle Scholar
  13. 13.
    Berger LR, Reynolds DM (1958) The chitinase system of a strain of Streptomyces griseus. Biochim Biophys Acta 29:522–534CrossRefGoogle Scholar
  14. 14.
    Farkaš V, Lišková M, Biely P (1985) Novel media for detection of microbial producers of cellulase and xylanase. FEMS Microbiol Lett 28(2):137–140CrossRefGoogle Scholar
  15. 15.
    Mishra S, Behera N (2008) Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes. Afr J Biotechnol 7(18):3326–3331Google Scholar
  16. 16.
    Berg G, Roskot N, Steidle A et al (2002) Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Appl J Environ Microbiol 68(7):3328–3338CrossRefGoogle Scholar
  17. 17.
    Fravel DR (1988) Role of antibiosis in the biocontrol of plant diseases. Annu Rev Phytopathol. 26:75–91CrossRefGoogle Scholar
  18. 18.
    Kim PI, Chung KC (2004) Production of an antifungal protein for control of Colletotrichum lagenarium by Bacillus amyloliquefaciens. FEMS Microbiol Lett 234:177–183CrossRefGoogle Scholar
  19. 19.
    Oppenheim AB, Chet I (1992) Cloned chitinase in fungal plant-pathogen control strategies. Trends Biotechnol 10:392–394CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Beenu Shastri
    • 1
  • Anil Kumar
    • 1
  • Rajesh Kumar
    • 1
  1. 1.Rhizosphere Biology Laboratory, Department of Environmental Microbiology, School for Environmental SciencesBabasaheb Bhimrao Ambedkar UniversityLucknowIndia

Personalised recommendations