Effect of Extraction Temperature and Different Carrier Agents on Physicochemical and Antioxidant Properties of Spray-Dried Murraya koenigii (Linn.) Leaf Extract

  • Vandana Sablania
  • Sowriappan John Don Bosco
  • Shubham Rohilla


The work evaluated the effect of extraction temperature on Murraya koenigii L. extract, prepared at an extraction temperature of 80 °C and 90 °C. The prepared Murraya koenigii L. leaf extract was encapsulated with different carrier agents such as guar gum, pectin, and whey protein isolates at a concentration of 1%, 5%, and 10% respectively. Spray drying was done at an inlet temperature of 130 °C with constant feed flow rate (8 rpm) at a pressure of 0.4 kg/cm2 and outlet temperature of 80 °C. The encapsulated powders were analyzed for their physicochemical properties such as moisture content, water activity, bulk density, tapped density, flow properties, antioxidant activity, and total phenolic content. The extract encapsulated with pectin and whey protein isolates did not show significant difference on DPPH activity at varied extraction temperature, whereas the extract encapsulated with guar gum showed more activity at an extraction temperature of 80 °C. The extract encapsulated with whey protein isolates showed more total phenolic content at an extraction temperature 90 °C. Therefore, it can be considered as a good source of active phytochemicals. Thus from this study, encapsulated Murraya koenigii L. extract could be considered as an important source of antioxidant and anti-inflammatory in human cells.


Murraya koenigii L. Spray drying Antioxidant activity Total phenolic content 



We acknowledge the Department of Food Science and Technology, Pondicherry University, Pondicherry, for providing facilities, and are also grateful to UGC for providing Junior Research Fellowship.


  1. 1.
    Singh AP (2011) LC-MS-MS characterization of curry leaf flavonols and antioxidant activity. Food Chem 127:80–85CrossRefGoogle Scholar
  2. 2.
    Ningappa MB, Dinesha R, Srinivas L (2008) Antioxidant and free radical scavenging activities of polyphenol-enriched curry leaf (Murraya koenigii L.) extracts. Food Chem 106:720–728CrossRefGoogle Scholar
  3. 3.
    Fernandes MRV, Azzolini AECS, Martinez MLL, Souza CRF, Lucisano-Valim YM, Oliveira WP (2014) Assessment of antioxidant activity of spray dried extracts of Psidium guajava leaves by DPPH and chemiluminescence inhibition in human neutrophils. BioMed Res Int Article ID 382891, 10 pagesGoogle Scholar
  4. 4.
    Santhalakshmy S, Bosco SJD, Francis S, Sabeena M (2015) Effect of inlet temperature on physicochemical properties of spray-dried jamun fruit juice powder. Powder Technol 274:37–43CrossRefGoogle Scholar
  5. 5.
    Association of Official Analytical Chemists (2006) Official methods of analysis of the Association of Official Analytical Chemists, 18th edn. AOAC Press, GaithersburgGoogle Scholar
  6. 6.
    Tonon VR, Brabet C, Hubinger M (2008) Influence of process conditions on the physicochemical properties of acai powder produced by spray drying. J Food Eng 88:411–418CrossRefGoogle Scholar
  7. 7.
    Goula AM, Adamopoulos KG, Kazakis NA (2004) Influence of spray drying conditions on tomato powder properties. Dry Technol 22(5):1129–1151CrossRefGoogle Scholar
  8. 8.
    Shah MA, Bosco SJD, Mir SA (2015) Effect of Moringa oleifera leaf extract on the physicochemical properties of modified atmosphere packaged raw beef. Food Packag Shelf Life 3:31–38CrossRefGoogle Scholar
  9. 9.
    Şahin Nadeem H, Torun M, Ozdemir F (2011) Spray drying of the mountain tea (Sideritis stricta) water extract by using different hydrocolloid carriers. LWT-Food Sci Technol 44:1626–1635CrossRefGoogle Scholar
  10. 10.
    Abadio FDB, Domingues AM, Borges SV, Oliveira VM (2004) Physical properties of powdered pineapple (Ananas comosus) juice: effect of malt dextrin concentration and atomization speed. J Food Eng 64(3):285–287CrossRefGoogle Scholar
  11. 11.
    Rao MA, Rizvi SSH, Datta AK (2005) Engineering properties of foods, 3rd edn. CRC Press, Boca RatonCrossRefGoogle Scholar
  12. 12.
    Paraskevopoulou A, Kiosseoglou V (2013) Interfacial properties of biopolymers, emulsions, and emulsifiers. In: Handbook of biopolymer-based materials: from blends and composites to gels and complex networks. Wiley, Weinheim, pp 717–740CrossRefGoogle Scholar
  13. 13.
    Ferrari CC, Germer SPM, Alvim ID, Vissotto FZ, de Aguirre JM (2012) Influence of carrier agents on the physicochemical properties of blackberry powder produced by spray drying. Int J Food Sci Technol 47:1237–1245CrossRefGoogle Scholar
  14. 14.
    Lebrun P, Krier F, Mantanus J, Grohganz H, Yang M, Rozet E, Boulanger B, Evrard B, Rantanen J, Hubert P (2012) Design space approach in the optimization of the spray-drying process. Eur J Pharm Biopharm 80:226–234CrossRefGoogle Scholar
  15. 15.
    Suhag Y, Nanda V (2015) Evaluation of different carrier agents with respect to physico-chemical, functional and morphological characteristics of spray dried nutritionally rich honey powder. J Food Process Preserv. ISSN 1745−4549Google Scholar
  16. 16.
    Flores FP, Singh RK, Kerr WL, Pegg RB, Kong F (2014) Total phenolics content and antioxidant capacities of microencapsulated blueberry anthocyanins during in vitro digestion. Food Chem 153:272–278CrossRefGoogle Scholar
  17. 17.
    Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, Mohana H (2003) Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemistry 63:97–104CrossRefGoogle Scholar
  18. 18.
    Cho M, Lee HS, Kang IJ, Won MH, You S (2011) Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chem 127:999–1006CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Vandana Sablania
    • 1
  • Sowriappan John Don Bosco
    • 1
  • Shubham Rohilla
    • 1
  1. 1.Department of Food Science and TechnologyPondicherry UniversityPuducherryIndia

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